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排序方式: 共有66条查询结果,搜索用时 15 毫秒
1.
Y Ohe ER Podack KJ Olsen Y Miyahara K Miura H Saito Y Koishihara Y Ohsugi T Ohira K Nishio 《Canadian Metallurgical Quarterly》1993,67(5):939-944
HuIL-6 cDNA, cloned into a neomycin resistant conferring expression vector, BMGNeo, was transfected into Lewis Lung Carcinoma (LLC) cells. LLC cells (5 x 10(6) ml-1) transfected with IL-6 cDNA (LLC-IL6) secreted IL-6 into the culture supernatant at a concentration of 9.9 ng ml-1 within 48 h. When 1,000,000 of untransfected LLC, BMGNeo vector transfected LLC (LLC-Neo) or LLC-IL6 cells were transplanted into C57BL/6 mice subcutaneously, the mean +/- s.d. of survival times of these mice were 33.3 +/- 9.7, 34.3 +/- 7.1 and 17.0 +/- 3.1 days, respectively. The survival time of LLC-IL6 cells transplanted mice was significantly shorter than that of LLC (P < 0.01) or LLC-Neo (P < 0.01) cells transplanted mice without a measurable difference of tumour size. Plasma concentration of IL-6 steadily increased in LLC-IL6 transplanted mice. Body weight and serum albumin were significantly lower in LLC-IL6 transplanted mice than in LLC transplanted mice. Mouse IL-1 alpha and mouse TNF-alpha were not detected in the plasma of LLC-IL6 transplanted mice. These data suggested that secretion of IL-6 from LLC cells was unable to alter net tumour growth rate but rather caused a state similar to cachexia without detectable increase of IL-1 alpha and TNF-alpha in the plasma. This state may be responsible for the shortened survival of LLC-IL6 tumour-bearing mice. 相似文献
2.
Shinichi Komaba Naoaki Yabuuchi Tomoaki Ozeki Koji Okushi Hiroharu Yui Kozo Konno Yasushi Katayama Takashi Miura 《Journal of power sources》2010,195(18):6069-6074
Poly(acrylic acid) (PAA), poly(methacrylic acid) (PMA), and poly(vinyl alcohol) (PVA), which have oxygen species as functional groups, were utilized as a binder for graphite electrodes, and the electrochemical reversibility of lithium intercalation was examined in PC medium and ionic liquid electrolyte, lithium bis(trifluoromethanesulfonyl)amide dissolved in 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)amide (BMP-TFSA). Columbic efficiency of 75–80% with more than 300 mAh g?1 was achieved upon first reduction/oxidation cycle in both electrolytes using these binding polymers, which were significantly improved in comparison to a conventional PVdF binder (less than 45% of columbic efficiency for the first cycle). For the graphite-PVdF electrode, co-intercalation and/or decomposition of PC molecules solvating to Li ions were observed by the electrochemical reduction, resulting in the cracking of graphite particles. In contrast, the co-intercalation and decomposition of PC molecules and BMP cations for the first reduction process were completely suppressed for the graphite electrodes prepared with the polymers containing oxygen atoms. It was proposed that the selective permeability of lithium ions was attained by the uniform coating of the graphite particles with PAA, PMA, and PVA polymers, because the electrostatic interaction between the positively charged lithium ions and negatively charged oxygen atom in the polymer should modulate the desolvation process of lithium ions during the lithium intercalation into graphite, showing the similar functions like artificial solid-electrolyte interphase. 相似文献
3.
Okamura T Takeno T Dohi M Yasumasa I Hayashi T Toyoda M Noda H Fukuda S Horie N Ohsugi M 《Journal of Bioscience and Bioengineering》2000,89(5):474-478
With thrombosis a major cause of death in Japan and the Western world, thrombin-inhibitory agents that constrain the formation of fibrin are sought. We screened for basidiomycetes showing anti-thrombin activity and isolated Laetiporus sulphureus. However, it was difficult to cultivate and its form was not satisfactory. We therefore used protoplast fusion between L. sulphureus and the commonly cultivated basidiomycete Hypsizygus marmoreaus to obtain cultivable basidiomycetes that produced an anti-thrombin substance. For the protoplast fusion of L. sulphureus and H. marmoreaus, the protoplast concentration, alternating electric field intensity, dielectrophoresis duration, and field pulse intensity used were of 1 x 10(7) protoplasts/ml, 100 V/cm.1 MHz, 60 s, and 8 kV/cm, respectively. The number of regenerated colonies obtained was 4961, from which 43 strains were selected for electrophoretic analysis. Four of the fusants were found to have a band from each parent in isozyme patterns obtained using their crude extract. The fruiting bodies of the fusants were very similar to those of H. marmoreaus. Crude extract from each of the fusants and from L. sulphureus showed anti-coagulative activity in terms of the thrombin clotting time. We thus obtained improved basidiomycetes that produce an anti-thrombin substance, are easily cultivated, and whose form resembles H. marmoreaus, a commonly used culinary mushroom. 相似文献
4.
S. Ohsugi Y. Kitaoka M. Azuma Y. Fujishiro M. Takano 《Journal of Low Temperature Physics》1999,117(5-6):1671-1675
We carried out the extensive Cu-nuclear magnetic and quadrupole resonance (NMR/NQR) experiments on the Zn(Ni)-doped ladder compound SrCu
2
O
3
(Sr123), Sr(Cu
1–x
M
x
)
2
O
3
(M=Zn and Ni) with x 0.02 and the Ladoped Sr123, Sr
1–x
La
x
Cu
2
O
3
with x 0.03. A spin-correlation length
s
/a (a: the lattice spacing between the Cu sites along the leg) of nonmagnetic impurity-induced staggered polarization (IISP) estimated from a quasi-one-dimensional (Q1D) IISP along the two legs in the 0.1–2 % Zn-doped Sr123 was found to be independent of temperature (T) and scaled to an mean impurity distance D
AV
with the relation of
s
/a = 2.5 + 0.1D
AV
The
s
/a's are much longer in x = 0.001 (
s
/a 50) and 0.005 (
s
/a 12) than an instantaneous spin-correlation length
0
/a 3 – 8 in Sr123. The formula of Néel T, T
N
(WC-Q1D)=J exp(–D
AV
/(s/a)) (J = 2000 K) based on the weakly interladder-coupled (WC) Q1D model explains the experimental T
N
values quantitatively. 相似文献
5.
H. Udono K. Nakamori Y. Takahashi Y. Ujiie I. J. Ohsugi T. Iida 《Journal of Electronic Materials》2011,40(5):1165-1170
We have investigated the crystal growth of single-phase MnSi1.75−x
by a temperature gradient solution growth (TGSG) method using Ga and Sn as solvents and MnSi1.7 alloy as the solute, and measured the thermoelectric properties of the resulting crystals. Single-phase Mn11Si19 and Mn4Si7 crystals were grown successfully using Ga and Sn as solvents, respectively. The typical size of a grown ingot of Mn11Si19 was 2 mm to 4 mm in thickness and 12 mm in diameter, whereas Mn4Si7 had polyhedral shape with dimensions in the range of several millimeters. The single-phase Mn11Si19 has good electrical conduction (ρ = 0.89 × 10−3 Ω cm to 1.09 × 10−3 Ω cm) compared with melt-grown multiphase higher-manganese silicide (HMS) crystals. The Seebeck coefficient, power factor,
and thermal conductivity were 77 μV K−1 to 85 μV K−1, 6.7 μW cm−1 K−2 to 7.2 μW cm−1 K−2, and 0.032 W cm−1 K−1, respectively, at 300 K. 相似文献
6.
7.
Poly(vinylamine), the simplest polycation with primary amines, was applied to interpenetrating polymer networks (IPN) with poly(acrylic acid). N-Vinylformamide (NVF) was employed for amino-protected monomers to control electrostatic balance. pH-responsivities of IPNs varied, depending on the hydrolysis conditions and acrylic acid (AAc) concentration of the second network. Poly(N-vinylacetamide)-co-poly(N-vinylformamide) (4/6, mol/mol) was employed for the first network, subsequently hydrolyzed with 50% amide groups, and the second network was polymerized with 0.25 mol L−1 AAc, extremely shrunken hydrogels with polyion complex were formed at pH 7, showing that the controlled amount of highly active primary amines are available in IPN. 相似文献
8.
To develop new polymer-based materials, the design of aliphatic carbonate has received attention and become a well-known cyclic monomer. In view of carbonate ring polymer scope, poly(trimethylene carbonate) (PTMC) has been continuously developed for further applications due to its unique degradability. PTMC bearing oligo ethylene glycol units, PTMCM-MOE3OM, were typically prepared via ring-opening polymerization (ROP) using amidine-based catalysts such as 1,8-diazabicyclo[5.4.0] undec-7-ene (DBU) and benzyl alcohol (BnOH) as an initiator. To improve the polymer molecular weight or other properties, several know-how synthetic catalysts based on organometallic complexes are under consideration as potential catalysts. With the existence of diverse classes of metallic complexes, the inorganic complexes were investigated for their catalytic activity based on tris(dimethylsilyl)amido chelating, bis(phenolate) chelating, and macrocyclic tetradentate (NNNN)-type cyclen chelating with a metal-core of tin (II), scandium (III), lutetium (III), and zinc (II). In this study, we found that involving a Zn(II) dimethylcyclen/alkoxide ligand and Mg complexes could accelerate the reaction and finish the polymerization under ambient conditions within 2 hr. Molecular weight reached 11,000 g/mol (40%) and 8,100 g/mol (> 96%). Subsequently, we concluded that Zn and Mg complexes were high reactivity for initiating the ROP of TMCM-MOE3OM upon steadily degree of polymerization. 相似文献
9.
Masaru Matsuoka Koichi Takagi Hiroharu Obayashi Kazuyuki Wakasugi Teijiro Kitao 《Coloration Technology》1983,99(9):257-260
Calculation of the n-electron excitation energies of naphthoquinonoid dyes has been carried out by means of the Pariser—Parr—Pople (PPP) method using the variable, β,γ-approximation. A good linear correlation between the observed and calculated values for the first π-π* excitation was found for a new series of naphthoquinonoid dyes. The effect of substituents on the colour of different dyes was studied on the basis of these calculated results, i.e. HOMO—LUMO energy levels and π-electron-density changes accompanying the excitation. The intramolecular charge-transfer character of the π-π* transition was shown to be the same as occurs in some anthraquinonoid dyes. 相似文献
10.