全文获取类型
收费全文 | 5775篇 |
免费 | 137篇 |
国内免费 | 26篇 |
专业分类
电工技术 | 441篇 |
综合类 | 17篇 |
化学工业 | 1544篇 |
金属工艺 | 145篇 |
机械仪表 | 147篇 |
建筑科学 | 196篇 |
矿业工程 | 1篇 |
能源动力 | 282篇 |
轻工业 | 521篇 |
水利工程 | 22篇 |
石油天然气 | 7篇 |
无线电 | 382篇 |
一般工业技术 | 1025篇 |
冶金工业 | 387篇 |
原子能技术 | 224篇 |
自动化技术 | 597篇 |
出版年
2023年 | 29篇 |
2022年 | 47篇 |
2021年 | 99篇 |
2020年 | 54篇 |
2019年 | 66篇 |
2018年 | 75篇 |
2017年 | 66篇 |
2016年 | 116篇 |
2015年 | 84篇 |
2014年 | 155篇 |
2013年 | 335篇 |
2012年 | 196篇 |
2011年 | 329篇 |
2010年 | 272篇 |
2009年 | 288篇 |
2008年 | 332篇 |
2007年 | 287篇 |
2006年 | 222篇 |
2005年 | 243篇 |
2004年 | 190篇 |
2003年 | 176篇 |
2002年 | 184篇 |
2001年 | 127篇 |
2000年 | 100篇 |
1999年 | 117篇 |
1998年 | 187篇 |
1997年 | 125篇 |
1996年 | 120篇 |
1995年 | 88篇 |
1994年 | 112篇 |
1993年 | 90篇 |
1992年 | 64篇 |
1991年 | 80篇 |
1990年 | 60篇 |
1989年 | 84篇 |
1988年 | 71篇 |
1987年 | 43篇 |
1986年 | 76篇 |
1985年 | 57篇 |
1984年 | 55篇 |
1983年 | 61篇 |
1982年 | 64篇 |
1981年 | 48篇 |
1980年 | 42篇 |
1979年 | 42篇 |
1978年 | 43篇 |
1977年 | 29篇 |
1976年 | 29篇 |
1975年 | 19篇 |
1973年 | 17篇 |
排序方式: 共有5938条查询结果,搜索用时 31 毫秒
1.
Masahiro Sato Miyu Koriyama Satoshi Watanabe Masato Ohtsuka Takayuki Sakurai Emi Inada Issei Saitoh Shingo Nakamura Kazuchika Miyoshi 《International journal of molecular sciences》2015,16(8):17838-17856
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of the above system is required. As a proof-of-concept, we attempted to disrupt a gene (GGTA1) encoding the α-1,3-galactosyltransferase that synthesizes the α-Gal epitope using parthenogenetically activated porcine oocytes. The lack of α-Gal epitope expression can be monitored by staining with fluorescently labeled isolectin BS-I-B4 (IB4), which binds specifically to the α-Gal epitope. When oocytes were injected with guide RNA specific to GGTA1 together with enhanced green fluorescent protein (EGFP) and human Cas9 mRNAs, 65% (24/37) of the developing blastocysts exhibited green fluorescence, although almost all (96%, 23/24) showed a mosaic fluorescent pattern. Staining with IB4 revealed that the green fluorescent area often had a reduced binding activity to IB4. Of the 16 samples tested, six (five fluorescent and one non-fluorescent blastocysts) had indel mutations, suggesting a correlation between EGFP expression and mutation induction. Furthermore, it is suggested that zygote microinjection of mRNAs might lead to the production of piglets with cells harboring various mutation types. 相似文献
2.
Shouko Nishida Masaya Shibano Hiroshi Kamitakahara Toshiyuki Takano 《International journal of molecular sciences》2015,16(12):29093-29102
We recently observed that the decanoylation of N-phenylthiocarbamoyl chitosan (2) with a mixture of decanoic anhydride and pyridine at 60 °C for 24 h afforded N,N-(decanoyl)phenythiocarbamoyl-/2-isothiocynato chitosan decanoate (3b) rather than the expected product N,N-(decanoyl)phenylthiocarbamoyl chitosan decanoate (3a). This result suggested that some of the N,N-(decanoyl)phenylthiocarmbamoyl groups had been converted to isothiocyanate groups during the decanoylation process. The subsequent reaction of compound 3b with aniline gave N,N-(decanoyl)phenylthiocarbamoyl/N-phenylthiocarbamoyl chitosan decanoate (4) in high yield. A solution of compound 4 in CHCl3 was then added to a solution of copper decanoate (5) in the same solvent, and the resulting mixture was cast onto a glass plate to give a cast film. The film was annealed at 200 °C in an oven to give a greenish film, which showed good near-infrared absorption characteristic in the range of 800–2200 nm. 相似文献
3.
4.
A Synthetic Transcriptional Activator of Genes Associated with the Retina in Human Dermal Fibroblasts
下载免费PDF全文
![点击此处可从《Chembiochem : a European journal of chemical biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
5.
Eicosapentaenoic acid (EPA) production by an oleaginous fungus Mortierella alpina expressing heterologous the Δ17‐desaturase gene under ordinary temperature
下载免费PDF全文
![点击此处可从《European Journal of Lipid Science and Technology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
6.
Hiroshi Takamatsu Hikaru Yamashiro Nobuo Takata Hiroshi Honda 《International Journal of Refrigeration》2003,26(6):79
Heat and mass transfer in a falling film vertical in-tube absorber was studied experimentally with LiBr aqueous solution. The presented results include the effect of solution flow rate, solution subcooling and cooling water temperature on the absorption in a smooth copper tube 16.05 mm I.D. and 400 mm long. The experimental data in the previous report for a 1200-mm-long tube was also re-examined and compared. It was demonstrated by the observation of the flow in the tube that the break down of the liquid film into rivulets leads to deterioration of heat and mass transfer at lower film Reynolds number or in longer tubes. An attempt to evaluate physically acceptable heat and mass transfer coefficients that are defined with estimated temperature and concentration at the vapor–liquid interface was also presented. 相似文献
7.
Single-polarization single-mode photonic crystal fibers 总被引:5,自引:0,他引:5
K. Saitoh M. Koshiba 《Photonics Technology Letters, IEEE》2003,15(10):1384-1386
A new structure of single-polarization single-mode (SPSM) photonic crystal fiber (PCF) is proposed and analyzed by using a full-vector finite element method with anisotropic perfectly matched layers. From the numerical results it is confirmed that the proposed fiber is low-loss SPSM-PCF within the wavelengths ranging from 1.48 to 1.6 /spl mu/m, where only the slow-axis mode exists and the confinement loss is less than 0.1 dB/km. 相似文献
8.
BaCuQF (Q=S, Se, Te) materials exhibit band gaps that allow transmission of much of the visible spectrum. BaCuSF is transparent in thin-film form with a band gap of 3.1 eV. Band gap estimates for powders of the solid solution series BaCuS1−xSexF were obtained from wavelength-dependent diffuse-reflectance measurements using an integrating sphere. The band gap can be tuned by the substitution of Se for S to 2.9 eV for BaCuSeF. The decrease scales almost linearly with the increase in the volume of the tetragonal unit cell, which is determined primarily by the expansion of the a lattice parameter; the overall volume increase is 7.0% from x=0 to 1. Further reduction of the band gap is observed in BaCuSe1−xTexF solid solutions, where a unit cell volume increase of 5.5% produces a band gap of 2.7 eV in BaCuSe0.5Te0.5F. Powders and films of BaCuSF exhibit strong red luminescence under ultraviolet excitation, which is suppressed by K doping. Additional tuning of band gap and electrical properties (the materials are p-type conductors) can be achieved by replacing Ba with Sr. 相似文献
9.
D Galasko T Saitoh Y Xia LJ Thal R Katzman LR Hill L Hansen 《Canadian Metallurgical Quarterly》1994,44(10):1950-1951
We determined apolipoprotein E (ApoE) genotypes in 122 autopsied demented patients. The frequency of the ApoE epsilon 4 allele was 39.6% in Alzheimer's disease (AD), 29.0% in the Lewy body variant of AD (LBV), and 6.25% in diffuse Lewy body disease. For AD and LBV patients, the epsilon 4 frequency was significantly higher than that reported in nondemented controls (10 to 15%). Therefore, LBV and AD share ApoE epsilon 4 as a genetic risk factor, providing further evidence that these conditions overlap. 相似文献
10.
K Ku K Nakayama Y Saitoh S Nosaka T Kitano T Hanada H Nagami K Yamada K Minami 《Canadian Metallurgical Quarterly》1997,64(2):399-403
Forty-eight Russell's viper bite patients (40 males, 8 females), age ranging from 16-76 years were studied. Out of 48 patients, 14 were found to have a prolonged whole blood clotting time test (WBCT) (i.e. incoagulable blood) (Group 1); 23 had a normal WBCT (i.e. clotted blood) (Group II); and 11 patients had a normal WBCT on admission which changed to non-clotting during the clinical course (Group III). Four patients from group I developed hypotension and 2 expired. The serum cortisol concentration (mean +/- SEM) on admission among groups I and II were 639 +/- 45.6 and 424 +/- 33.2 nmol/l respectively. The blood cortisol level in 35 subjects (controls) were 370.7 +/- 17.7 nmol/l (mean +/- SEM). There was a significant rise of blood cortisol in patients with incoagulable blood when compared to controls at the time of admission to the hospital (p < 0.05); but there was no significant difference among those patients with clotted blood. A much higher mean serum cortisol level was observed in 4 patients with hypotension as compared to 10 patients without shock. These patients with hypotension according to our study shown to have a favorable response to steroid therapy and eventually recovered. Whether higher doses of steroid in addition to antiserum confer extra benefit in suppressing nonspecific venom effects on the pituitary and/or adrenal is not known. 相似文献