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P Fiset HL Lemmens TD Egan SL Shafer DR Stanski TE Egan 《Canadian Metallurgical Quarterly》1995,58(5):567-582
The purpose of this study was to model pharmacodynamically the reversal of midazolam sedation with flumazenil. Ten human volunteers underwent four different sessions. In session 1, individual midazolam pharmacokinetics and electroencephalographic pharmacodynamics were determined. In sessions 2 and 3, a computer-controlled infusion of midazolam with individual volunteer pharmacokinetic data was administered, targeting a plasma concentration corresponding to a light or deep level of sedation (20% or 80% of the maximal midazolam electroencephalographic effect) for a period of 210 minutes. After obtaining a stable electroencephalographic effect and constant midazolam plasma concentrations, a zero-order infusion of flumazenil was started until complete reversal of midazolam electroencephalographic effect was obtained. The flumazenil infusion was then stopped and the volunteer was allowed to resedate because of the constant midazolam drug effect. The electroencephalographic response was measured during a 180-minute period and analyzed by aperiodic analysis and fast-Fourier transforms. In session 4, a midazolam plasma concentration corresponding to a deep level of sedation was targeted for 210 minutes to examine for the possible development of acute tolerance. No flumazenil was given in session 4. For a light sedation level, with a mean midazolam plasma concentration of 160 +/- 64 ng/ml, the mean half-life of the equilibration rate constant of flumazenil reversal is 5.0 +/- 2.5 minutes, and the mean effect site concentration causing 50% of Emax is 13.7 +/- 5.8 ng/ml. For a deep level of sedation, with a mean midazolam plasma concentration of 551 +/- 196 ng/ml, the mean half-life of the equilibration rate constant is 3.9 +/- 1.5 minutes, and the mean effect site concentration causing 50% of Emax is 20.6 +/- 6.8 ng/ml. This study provides an estimate of the magnitude of the blood/central nervous system equilibration delay for flumazenil antagonism of midazolam sedation and further defines the usefulness of the electroencephalogram as a measure of midazolam pharmacodynamic effect. 相似文献
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We report isolating the Drosophila retinal degeneration E (rdgE) mutation. The hypomorphic rdgE1 allele causes rapid photoreceptor degeneration in light and a slower rate of degeneration when the flies are raised in constant darkness. The rdgE1 flies exhibited an electrophysiological light response that decreased with age, coinciding with the degeneration. This suggests that degeneration caused the loss of the light response. We determined that the ninaE (rhodopsin) mutation, but not norpA [phospholipase C (PLC)], slowed the rdgE-dependent degeneration. This was consistent with the light-enhanced degeneration, but revealed that the degeneration is independent of the PLC-mediated phototransduction cascade. Transmission electron microscopy revealed that rdgE1 photoreceptors exhibited a number of vesicular transport defects including unpacking/vesiculation of rhabdomeres, endocytosis of novel vesicles by photoreceptors, a buildup of very large multivesicular bodies, and an increased amount of rough endoplasmic reticulum. We determined that the rdgE null phenotype is a late embryonic lethality. Therefore, rdgE+ is required in cells outside of the retina, quite possibly in a large number of neurons. Thus, rdgE may define a mutational class that exhibits both light-enhanced retinal degeneration and a recessive null lethality by perturbing neuronal membrane biosynthesis and/or recycling. 相似文献
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This study examined the effects of instructional match and content overlap on students' ability to generalize from passage reading instruction. Four students with mild disabilities served as participants. Using a multielement design, students were instructed with passages at two levels of text difficulty (instructionally matched vs. instructionally mismatched), and generalization was assessed with passages at two levels of similarity to those instructed (low vs. high content overlap). Results indicated that students' oral reading accuracy and fluency showed the greatest degree of generalization when instructional materials were matched to the students' skill level and assessment materials were similar to those used during instruction. Moreover, these results were maintained at 1-month follow-up. The implications of these findings for classroom reading instruction and the assessment of students' reading skills are discussed. 相似文献
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Effect of microstructure on the stability of retained austenite in transformation-induced-plasticity steels 总被引:2,自引:0,他引:2
I. B. Timokhina P. D. Hodgson E. V. Pereloma 《Metallurgical and Materials Transactions A》2004,35(8):2331-2341
Two Fe-0.2C-1.55Mn-1.5Si (in wt pct) steels, with and without the addition of 0.039Nb (in wt pct), were studied using laboratory
rolling-mill simulations of controlled thermomechanical processing. The microstructures of all samples were characterized
by optical metallography, X-ray diffraction (XRD), and transmission electron microscopy (TEM). The microstructural behavior
of phases under applied strain was studied using a heat-tinting technique. Despite the similarity in the microstructures of
the two steels (equal amounts of polygonal ferrite, carbide-free bainite, and retained austenite), the mechanical properties
were different. The mechanical properties of these transformation-induced-plasticity (TRIP) steels depended not only on the
individual behavior of all these phases, but also on the interaction between the phases during deformation. The polygonal
ferrite and bainite of the C-Mn-Si steel contributed to the elongation more than these phases in the C-Mn-Si-Nb-steel. The
stability of retained austenite depends on its location within the microstructure, the morphology of the bainite, and its
interaction with other phases during straining. Granular bainite was the bainite morphology that provided the optimum stability
of the retained austenite. 相似文献
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A system has been developed for tracking the motion of objects in two dimensions in real-time. The system consists of a conventional CCD camera linked to a transputer-based frame grabber and an array of nine transputers. A parallel moments algorithm is used to extract the co-ordinates of the object's centre of gravity and orientation at field rate, i.e. 60 Hz. Since the position data are made available in real-time—with a small time delay—the system has the potential for inclusion in a feedback loop. Results are presented for tracking the trajectory of a chocolate bar diverted by an air jet. The potential of the system for higher sampling rates—up to 200 Hz—is discussed. 相似文献
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Hodgson A.J. Hogan N. 《IEEE transactions on systems, man and cybernetics. Part C, Applications and reviews》2000,30(1):105-118
Both in designing teleoperators or haptic interfaces and in fundamental biological motor control studies, it is important to characterize the motor commands and mechanical impedance responses of the operator (or subject). Although such a characterization is fundamentally impossible for isolated movements when these two aspects of motor behavior have similar time scales (as is the case with humans), it is nonetheless possible, if we are dealing with repeated movements, to measure a trajectory which is analogous to the current source in Norton-equivalent electrical circuits. We define the attractor trajectory to be this equivalent source and show that it rigorously embodies the notion of the attractor point of a time-evolving system. We demonstrate that most previous attempts to test a controversial motor control hypothesis known as the “equilibrium point” or “virtual trajectory” hypothesis are based on inadequate models of the neuromuscular system, and we propose a model-independent means of testing the hypothesis based on a comparison of measurable attractor trajectories at different levels of the motor system. We present and demonstrate means of making such measurements experimentally and of assigning error bounds to the estimated trajectories 相似文献