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Blondel Arnaud; Nageotte Roland; Bedouelle Hugues 《Protein engineering, design & selection : PEDS》1996,9(2):231-238
Hybrid MalEGVP is a bifunctional protein in vitro sinceit binds maltose as protein MalE of Escherichia coli and sinceit is dimeric and specifically binds single-stranded DNA asprotein GVP of phage M13. The oxidation rate of a unique cysteineresidue was used to compare the stabilities of GVP in its freeand hybrid forms, under conditions where MalE was either foldedor unfolded by a denaturing agent. The results showed that boththe covalent link and tertiary non-covalent interactions betweenMalE and GVP destabilized GVP in MalEGVP. To test whetherGVP had identical structures in its free and hybrid forms, mutationswere used as local conformational probes. The effects of thesemutations on the capabilities of MalEGVP to dimerizeand to bind single-stranded DNA were assayed in vitro. Theywere compatible with the effects of the same mutations on theglobal activity of free GVP in vivo and with the effects thatcould be predicted from the known data on free GVP, in particularits crystal structure. Thus, one partner of a hybrid proteincan be destabilized by the other partner while maintaining itsstructural and functional characteristics. 相似文献
3.
Hugues Duncan 《Electrochimica acta》2007,52(21):6195-6205
Hydrogen adsorption and absorption at thin palladium deposits of 0.8-10 monolayers (ML) on Au(1 1 1) was studied in 0.1 M H2SO4 and HClO4 using cyclic voltammetry, ac voltammetry, and impedance spectroscopy in the absence and in the presence of poison, crystal violet. Hydrogen adsorption on palladium is more reversible in sulfuric acid than in perchloric acid but it occurs at potentials 30 mV more positive in latter. The charge-transfer resistance exhibits a minimum at ∼0.27 V versus RHE and decreases with increasing in Pd deposit thickness in both acids. Adsorption capacitance at 0.8 ML Pd reaches maximum at the same potential. At other deposits the pseudo-capacitance starts to increase at lower overpotentials indicating the beginning of absorption, even at 2 ML Pd. The double layer capacitance is similar for all the deposits in sulfuric acid and it has a sharp maximum at 0.27 V versus RHE. In perchloric acid a broad maximum is observed. Crystal violet inhibits hydrogen adsorption but makes hydrogen absorption more reversible. The results suggest a fast direct hydrogen absorption mechanism that proceeds in parallel with slower hydrogen adsorption and indirect absorption. 相似文献
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We present a mathematical analysis of the effects of Hebbian learning in random recurrent neural networks, with a generic Hebbian learning rule, including passive forgetting and different timescales, for neuronal activity and learning dynamics. Previous numerical work has reported that Hebbian learning drives the system from chaos to a steady state through a sequence of bifurcations. Here, we interpret these results mathematically and show that these effects, involving a complex coupling between neuronal dynamics and synaptic graph structure, can be analyzed using Jacobian matrices, which introduce both a structural and a dynamical point of view on neural network evolution. Furthermore, we show that sensitivity to a learned pattern is maximal when the largest Lyapunov exponent is close to 0. We discuss how neural networks may take advantage of this regime of high functional interest. 相似文献
6.
Medial axes and skeletons are notoriously sensitive to contour irregularities. This lack of stability is a serious problem for applications in e.g. shape analysis and recognition. In 2005, Chazal and Lieutier introduced the λ-medial axis as a new concept for computing the medial axis of a shape subject to single parameter filtering. The λ-medial axis is stable under small shape perturbations, as proved by these authors. In this article, a discrete λ-medial axis (DLMA) is introduced and compared with the recently introduced integer medial axis (GIMA). We show that DLMA provides measurably better results than GIMA, with regard to stability and sensibility to rotations. We give efficient algorithms to compute the DLMA, and we also introduce a variant of the DLMA which may be computed in linear-time. 相似文献
7.
We first define the basic notions of local and non-local tasks for distributed systems. Intuitively, a task is local if, in a system with no failures, each process can compute its
output value locally by applying some local function on its own input value (so the output value of each process depends only
on the process’ own input value, not on the input values of the other processes); a task is nonlocal otherwise. All the interesting
distributed tasks, including all those that have been investigated in the literature (e.g., consensus, set agreement, renaming,
atomic commit, etc.) are non-local. In this paper we consider non-local tasks and determine the minimum information about
failures that is necessary to solve such tasks in message-passing distributed systems. As part of this work, we also introduces
weak set agreement—a natural weakening of set agreement—and show that, in some precise sense, it is the weakest nonlocal task in message-passing systems. 相似文献
8.
This letter aims at studying the impact of iterative Hebbian learning algorithms on the recurrent neural network's underlying dynamics. First, an iterative supervised learning algorithm is discussed. An essential improvement of this algorithm consists of indexing the attractor information items by means of external stimuli rather than by using only initial conditions, as Hopfield originally proposed. Modifying the stimuli mainly results in a change of the entire internal dynamics, leading to an enlargement of the set of attractors and potential memory bags. The impact of the learning on the network's dynamics is the following: the more information to be stored as limit cycle attractors of the neural network, the more chaos prevails as the background dynamical regime of the network. In fact, the background chaos spreads widely and adopts a very unstructured shape similar to white noise. Next, we introduce a new form of supervised learning that is more plausible from a biological point of view: the network has to learn to react to an external stimulus by cycling through a sequence that is no longer specified a priori. Based on its spontaneous dynamics, the network decides "on its own" the dynamical patterns to be associated with the stimuli. Compared with classical supervised learning, huge enhancements in storing capacity and computational cost have been observed. Moreover, this new form of supervised learning, by being more "respectful" of the network intrinsic dynamics, maintains much more structure in the obtained chaos. It is still possible to observe the traces of the learned attractors in the chaotic regime. This complex but still very informative regime is referred to as "frustrated chaos." 相似文献
9.
In this paper, we propose a method for real-time horizon tracking (i.e., separation line between the sky and the sea) in a maritime operations context. We present the fusion of an image processing algorithm with the data obtained from the inertial measurement unit (IMU). The initial aim is to filter out environmental conditions using inertial information in order to combine a video stream with onboard electronic charts. This is achieved by the detection of the horizon with an image processing algorithm in an area defined by the IMU. We then present an evaluation of the algorithm with regard to the rate of detection of the horizon and the impact of the image resolution on the computational time. The purpose of developing this method is to create an augmented reality maritime operations application. We combine the video stream with electronic charts in a single display. We use the position of the horizon in the image to split the display into different areas. Then, we use transparency to display the video, the electronic charts or both. 相似文献
10.
Edouard Chauveau Catherine Marestin Régis Mercier Alexis Brunaux Vincent Martin Ricardo P. Nogueira Aurélien Percheron Virginie Roche Hugues Waton 《应用聚合物科学杂志》2015,132(17)
As a part of research work to elaborate polymeric materials for metal corrosion protection, we have developed a new family of phosphonic acid‐containing polymers. The synthesis and the characterization of polysulfones bearing alkyl phosphonate ester side groups are first described. These polymers are synthesized by direct polycondensation of a phosphonate ester‐containing bisphenol by aromatic nucleophilic substitution. The physicochemical properties of the resulting polymers are described. Acidic hydrolysis of phosphonate esters results in the formation of phosphonic acid groups. A series of phosphonic acid‐containing polysulfones is therefore obtained and characterized. A preliminary evaluation of the anticorrosive properties of these polymers is described. In 0.25M Na2SO4 solution, the corrosion rate of a polymer‐coated mild steel sample is much lower than of the free metal substrate. These results suggest that phosphonic acid‐containing polysulfones might be interesting as anticorrosive coatings. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41890. 相似文献