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排序方式: 共有338条查询结果,搜索用时 15 毫秒
1.
2.
Cell recognition, signal induction, and symmetrical gene activation at the dorsal-ventral boundary of the developing Drosophila wing 总被引:2,自引:0,他引:2
Appendage formation in insects and vertebrates depends upon signals from both the anterior-posterior and dorsal-ventral (DV) axes. In Drosophila, wing formation is organized symmetrically around the DV boundary of the growing wing imaginal disc and requires interactions between dorsal and ventral cells. Compartmentalization of the wing disc, dorsal cell behavior, and the expression of two dorsally expressed putative signaling molecules, fringe (fng) and Serrate (Ser), are regulated by the apterous selector gene. Here, we demonstrate that fng and Ser have distinct roles in a novel cell recognition and signal induction process. fng serves as a boundary-determining molecule such that Ser is induced wherever cells expressing fng and cells not expressing fng are juxtaposed. Ser in turn triggers the expression of genes involved in wing growth and patterning on both sides of the DV boundary. 相似文献
3.
4.
Sumathy Arumuganathar Scott Irvine Jean R. McEwan Suwan N. Jayasinghe 《应用聚合物科学杂志》2008,107(2):1215-1225
In a recent discovery, coaxial electrospinning was explored to encapsulate living organisms within a continuous bio‐polymeric microthread from which active biological scaffolds were fabricated (Townsend‐Nicholson and Jayasinghe, Biomacromolecules 2006, 7, 3364). The cells were demonstrated to have gone through all expected cellular activity without their viability being compromised. These biologically active threads and scaffolds have direct and tremendous applicability from regenerative to therapeutic medicine. Currently these post‐processed cells as composite threads and scaffolds are being investigated in‐depth at a cellular level to establish if the processing methodology has any affect on the cellular make‐up. We now demonstrate a competing non‐electric field driven approach for fabricating composite threads and scaffolds influenced only by a differential pressure. We refer to this novel composite thread to scaffold fabrication methodology as coaxial aerodynamically assisted bio‐threading (CAABT). Our investigations firstly, demonstrate that this technique can process handle living organisms without biologically perturbing them in anyway. Secondly the process is elucidated as possessing the ability to form composite active threads from which biologically viable scaffolds are formed. Finally our study employs florescent activated cell sorting (FACScan), a method by which the cellular dynamics and viability are quantified on control and threaded cellular samples at two prescribed time points. In parallel with FACScan, optical comparison of cellular morphology at three time points within a period of three weeks is carried out to photographically observe any changes in the post‐processed cellular phenotype. Our developmental investigations into this novel aerodynamically assisted threading methodology has unearthed a unique biomicrofabrication approach, which joins cell electrospinning in the cell threading to scaffold fabrication endeavor. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2008 相似文献
5.
S. J. C. Irvine A. Stafford M. U. Ahmed A. Brown H. Kheyrandish 《Journal of Electronic Materials》1997,26(6):723-727
The first detailed comparison has been made of the metalorganic vapor phase epitaxy growth rates of CdTe, ZnTe, and ZnSe,
measured in situ with laser reflectometry. The comparison also includes the photo-assisted growth with visible radiation from
an argon ion laser. Using a standard Group II precursor (DMCd or DMZn.TEN) partial pressure of 1.5 × 10−4 atm, VI/II ratio of 1 and DIPM (M = Te, Se) the maximum growth rates are in the region of 10 to 15 AU/ s. Decrease in growth
rates of ZnTe at higher temperatures or higher laser powers have been attributed to the desorption from the substrate of unreacted
Te precursor. The behavior of DTBSe is quite different from DIPSe for both pyrolytic and photo-assisted growth. The maximum
growth rate is around 1 AU/ s with very little photo-enhancement, except at 300°C. Secondary ion mass spectroscopy analysis
of hydrogen concentration in the ZnSe layers shows high concentrations, up to 5.9 × 1019 atoms cm−3 for DTBSe grown ZnSe under pyrolytic conditions. These results show that the growth kinetics play an important part in the
incorporation of hydrogen and passivation of acceptor doped material. 相似文献
6.
J. Bajaj S. J. C. Irvine H. O. Sankur Spyros A. Svoronos 《Journal of Electronic Materials》1993,22(8):899-906
An effective way to in situ monitor the metalorganic chemical vapor deposition (MOCVD) of HgCdTe/CdTe/ZnTe on GaAs or GaAs/Si
substrates is presented. Specular He-Ne laser reflectance was used to in situ monitor the growth rates, layer thickness, and
morphology for each layer in the grown multilayer structure. In situ monitoring has enabled precise measurements of ZnTe nucleation
and CdTe buffer layer thicknesses. Monitoring the constancy of reflectance during the thicker CdTe buffer growth where absorption
in the CdTe reduces reflectance to just the surface component has led to optimum buffer growth ensuring good quality of subsequently
grown HgCdTe. During the interdiffused multilayer process (IMP) HgCdTe growth, because multiple interfaces are present within
the absorption length, a periodic reflectance signal is maintained throughout this growth cycle. A theoretical model was developed
to extract IMP layer thicknesses from in situ recorded experimental data. For structures that required the growth of a larger
band gap HgCdTe cap layer on top of a smaller band gap active layer, in situ monitored reflectance data allowed determination
of alloy composition in the cap layer as well. Continuous monitoring of IMP parameters established the stability of growth
conditions, translating into depth uniformity of the grown material, and allowed diagnosis of growth rate instabilities in
terms of changes in the HgTe and CdTe parts of the IMP cycle. A unique advantage of in situ laser monitoring is the opportunity
to perform “interactive” crystal growth, a development that is a key to real time MOCVD HgCdTe feedback growth control. 相似文献
7.
Effects of hyperlipemia on circulating leukocytes and erythrocytes were studied in dogs which were given repeated, intravenous
injections of a nonionic detergent, Triton WR-1339.
Erythrocyte lipid concentrations increased from 3.6±0.9 mg/1010 cells in control animals to 9.3±1.5 mg in the hyperlipemic group. This increase was accompanied by a shift in the distribution
of total fatty acids toward a higher percentage of saturated and monounsaturated acids. In contrast to the changes observed
in erythrocytes, the leukocyte lipid content remained unaltered in dogs with serum cholesterol levels ranging from 500 to
more than 2,000 mg/100 ml. Leukocyte counts rose whereas hematocrit values, hemoglobin concentrations, and erythrocyte counts
decreased. Oxygen utilization studies showed no significant metabolic differences between leukocytes which were isolated from
hyperlipemic or control animals. Circulating leukocytes in dogs with an endogenously induced hyperlipemia were shown therefore
to maintain normal lipid concentrations and did not participate in lipophage formation, as reported for certain diet-induced
lipemias. 相似文献
8.
B. M. Craig T. M. Mallard R. E. Wight G. N. Irvine J. R. Reynolds 《Journal of the American Oil Chemists' Society》1973,50(10):395-399
Single plant isolates in theBrassica napus andBrassica campestris species of rapeseed yielded glyceride oil containing small amounts of erucic acid. Agronomically suitable varieties were
grown commercially in 1971 as the first phase in a changeover of Canadian rapeseed production from varieties with erucic contents
of 20–45% to low erucic acid varieties. A program to monitor the erucic content by gas chromatographic analysis in the stages
of production, handling and transportation from seed to export shipment was undertaken to evaluate the effects of genetics,
environment and admixture. The individual increase in erucic content ranged from 0.5 to 1.0, resulting in total increases
of 1–2%.
NRCC No. 13471.
One of six papers presented in the symposium “Rapeseed Marketing and Breeding,” AOCS Meeting, Ottawa, September 1972. 相似文献
9.
In the paper, the characters and fields were introduced for utilizing the Series/Parallel Switch (SPS). The circuit principle of the SPS technology was analyzed in detailed, explained and illuminated. We understood clearly the difference between SPS and single-phase transformator by the figure and table. Finally, it summarized that the main characters of SPS. 相似文献
10.
Why do fish strand? An analysis of ten years of flow reduction monitoring data from the Columbia and Kootenay rivers,Canada 下载免费PDF全文
Stranding of fish due to flow reductions has been documented in the near shore of the Columbia and Kootenay Rivers, Canada, and can result in sub‐lethal or lethal effects on fish. Ten years (1999–2009) of monitoring data have been collected at sites below two hydro‐electric dams (Hugh‐L‐Keenleyside and Brilliant Dam) following flow reductions. A generalized linear mixed effects model analysed the probability of a stranding event in relation to environmental and operational variables including the rate of change in the water levels, the duration of shoreline inundation prior to a reduction (wetted history), the river stage, the magnitude of the reduction, distance downstream from the dam, time of day, day of year (season) and whether a site had been physically altered to mitigate stranding. The results demonstrated statistically significant effects on stranding risk from minimum river stage, day of the year and whether a site had been physically re‐contoured. The combination of investigated factors giving the highest probability of stranding was a large magnitude reduction completed in the afternoon in midsummer, at low water levels when the near shore had been inundated for a long period. This research is significant in its approach to assessing years of ecosystem scale monitoring data and using the modelling results to determine ways for these findings to be applied in regulated river management to minimize fish stranding. It also highlighted data gaps that require addressing and provides ecosystem scale results to compare with stranding studies carried out in mesocosms. © 2014 The Authors. River Research and Applications published by John Wiley & Sons Ltd. 相似文献