A Study of Quartzites of the Koitashskoe Deposit for Their Applicability to the Production of Dinas Parts

Various samples of quartzite of the Koitashskoe deposit are studied for checking their applicability as a raw material for the production of dinas. In accordance with the degree of devolution, the refractoriness, the heat resistance, the water absorption, and the porosity, quartzites of the Koitashskoe deposit meet the requirements of the GOST 9854–81 standard and can be used for the production of dinas parts.     

High-power semiconductor radiation sources based on 100-W diode laser bars for pumping solid-state lasers

High-power semiconductor radiation sources with a total optical output power of up to 5 kW and a power density of 400 W/cm² have been developed, representing a vertical stack of quasi-continuous pulsed (500 μs) 100-W diode laser bars. Based on such sources, a solid-state laser with an output radiation energy of up to 150 mJ has been created, which is intended for information systems, laser radars, spectrum analyzers, etc.     

Double chamber solar and fuel drying unit for agricultural products

A service model of a combined double chamber (casing) solar and fuel vertical drying unit was developed based on theoretical and experimental investigations.     

Optical properties of a polyimide-fullerene system in the near infrared range (λ=1047 nm)

The optical properties of polyimide sensitized with fullerenes C₆₀ and C₇₀ have been studied for the first time in the near infrared range. It is shown that materials based on the polyimide-fullerene system can be used for limitation of the laser radiation power in this spectral range.     

Cloning and Characterization of the ∆6 Polyunsaturated Fatty Acid Elongase from the Green Microalga <Emphasis Type="Italic">Parietochloris incisa</Emphasis>

The very-long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid (ARA, 20:4ω-6) is a component of neuron tissues such as brain and retina cells and a primary substrate for the biosynthesis of biologically active eicosanoids. The green freshwater microalga Parietochloris incisa (Trebouxiophyceae) has been shown to accumulate an extraordinary high content of ARA-rich triacylglycerols. It was thus interesting to characterize the genes involved in lipid biosynthesis in this alga. We report here the identification of a cDNA encoding for a P. incisa PUFA elongase (PiELO1) and demonstrate that the expression of PiELO1 in yeast Saccharomyces cereviseae confers its elongase activity on C18 ∆6 PUFA. Phylogenetic analysis indicated that PiELO1 is highly similar to functionally characterized ∆6 PUFA elongase genes from other green algae and lower plants. Quantitative real-time PCR expression studies showed that PiELO1 is upregulated under nitrogen starvation, the condition triggering and enhancing storage oil and ARA accumulation in P. incisa.     

Highly efficient high-power quasi-continuous diode laser bars for pumping solid-state lasers based on Yb-containing active media

Highly efficient high-power quasi-continuous diode laser bars (DLBs) emitting in the region of λ=0.95 μm have been developed for pumping solid-state lasers based on Yb-containing active media. The parameters of DLBs and the results of long-term tests are presented. The proposed DLBs are implemented in a solid-state laser based on an Yb-Er glass operating in the 1.5 mm range.     

Identification and Characterization of Δ12, Δ6, and Δ5 Desaturases from the Green Microalga <Emphasis Type="Italic">Parietochloris incisa</Emphasis>

The freshwater microalga Parietochloris incisa accumulates, under nitrogen starvation, large amounts of triacylglycerols containing approximately 60% of the ω6 very long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid. Based on sequence homology, we isolated three cDNA sequences from P. incisa, designated PiDesD12, PiDesD6, PiDesD5. The deduced amino acid sequences of the three genes contained three conserved histidine motifs; the front-end desaturases, PiDes6 and PiDes5, contained a fused N-terminal cytochrome b5 domain. By functional characterization in the yeast Saccharomyces cerevisiae, we confirmed that PiDesD6, PiDesD5 cDNA encode membrane bound desaturases with Δ6, and Δ5 activity, respectively. Both PiDes6 and PiDes5 can indiscriminately desaturate both ω6 and ω3 substrates. A phylogenetic analysis showed that the three genes were homologous to the corresponding desaturases from green microalgae and lower plants that were functionally characterized. Quantitative real-time PCR revealed the concerted expression pattern of all three genes in P. incisa cells subjected to nitrogen starvation, featuring maximum expression level on day 3 of starvation, corresponding to the sharpest increase in the share of arachidonic acid.