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Daniela M. Nevskaia Maria Luisa Rojas Cervantes Antonio Guerrero Ruíz Juan de Dios Lpez Gonzlez 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1995,63(3):249-256
Adsorption of Triton X-100 on various silica substrates has been investigated. A number of solids, including a natural quartz, this quartz washed with HCl acid and subsequently heated at 1273 K; two aerosils and one Kieselgel silicas were studied. These solids exhibit surface areas in the range of 5 to 430 m2 g?1. All the Triton adsorption isotherms display an S-shape at the adsorption temperatures studied (298 and 308 K). It has been found that the pretreatments of natural quartz (by water washing, impurities removed by acid and/or high temperature calcination) affect considerably the amounts of TX-100 adsorbed. Measurements of surface composition have been made by X-ray photoelectron spectroscopy (XPS) with particular emphasis on the presence of impurities and on the number of OH groups at the surface of the samples. The nature of the surface hydroxyl has also been studied by infrared spectroscopy. Furthermore, the specific number of hydroxyl groups on the surface of the silica samples has been determined by thermogravimetric analysis. Finally an attempt to correlate solid surface characteristics with adsorption isotherms has been developed. 相似文献
4.
MP Romero A García-Perga?eda JM Guerrero C Osuna 《Canadian Metallurgical Quarterly》1998,12(13):1401-1408
Melatonin has been suggested as a physiological antagonist of calmodulin. In this work, we have characterized melatonin binding sites in Xenopus laevis oocyte membranes. Binding of [125I]melatonin by X. laevis oocyte membranes fulfills all criteria for binding to a receptor site. Binding was dependent on time, temperature, and membrane concentration and was stable, reversible, saturable, and specific. The binding site was also pharmacologically characterized. Stoichiometric studies showed a high-affinity binding site with a Kd of 1.18 nM. These data are in close agreement with data obtained from kinetic studies (Kd=0.12 nM). In competition studies, we observed a low-affinity binding site (Kd=63.41 microM). Moreover, the binding site was characterized as calmodulin. Thus, binding was dependent on calcium and blocked by anti-CaM antibodies in a concentration-dependent manner. Calmodulin inhibitor chlorpromazine also inhibited binding of the tracer. From these results, it is suggested that membrane-bound calmodulin acts as a melatonin binding site in Xenopus laevis oocytes, where it might couple cellular activities to rhythmic circulating levels of melatonin. This hypothesis correlates with the previous findings describing melatonin as a physiological antagonist of calmodulin. 相似文献
5.
María Pinto Dora Sales Anne-Vinciane Doucet Andrés Fernández-Ramos David Guerrero 《Scientometrics》2007,72(2):345-370
The purpose of the study proposed in this paper is to evaluate the Spanish public university websites dedicated to the European
Higher Education Area (EHEA). To do so, the quality of these resources has been analysed in the light of data provided by
a series of indicators grouped in seven criteria, most of which were used to determine what information is made available
and in what way. The criteria used in our analysis are: visibility, authority, updatedness, accesibility, correctness and
completeness, quality assessment and navigability.
All in all, the results allow us to carry out an overall diagnosis of the situation and also provide us with information about
the situation at each university, thus revealing their main strengths, namely authority and navegability, and also their chief
shortcomings: updatedness, accessibility and quality assessment. In this way it is possible to detect the best practices in
each of the aspects evaluated so that they can serve as an example and guide for universities with greater deficiencies and
thus help them to improve their EHEA websites. 相似文献
6.
JC Hernández Guerrero J Portilla Robertson C Ledezma Montes S Ponce-Bravo A Miranda Gómez EM Arias Rivera 《Canadian Metallurgical Quarterly》1996,44(1-4):25-30
Several forms of cell perturbation have been associated with ethanol ingestion. Fetal alcohol syndrome (FAS) as well as diminished maxillofacial development and inhibition of cell regeneration in vitro and in vivo have been described. Epidermal growth factor (EGF) stimulates maxillofacial growth, DNA synthesis, and it is a potent mitogen for a number of various cell types. EGF exerts its effects on cells through binding to a specific cell surface receptor which leads to activation of a thyrosine kinase in the intracellular part of the receptor. The inhibitory effect of alcohol on EGF in the mouse dental follicle was studied in the offspring of alcoholic mothers using immunocytochemistry. Adult female mice were given 22% alcohol in their drinking water and fed a pelleted diet before and during pregnancy. Maternal blood alcohol levels were 262 +/- 1.3 mg/100 ml on gestation day 12.5. The offspring of the alcoholic and control mice were sacrificed on postnatal day 1.5, their mandibles were dissected, weighed and processed by routine immunocytochemistry with the following results. 1) Significant differences were found in mandible weight p < 0.01 after parturition. 2) The tooth germs in the offspring of ethanol treated mice were morphometrically smaller than those of control littermates. 3) Immunoexpression of EGF in the mandibular first molar of the control group was strong and homogeneous while in the experimental group the expression was light and heterogeneous. It is concluded that maternal alcoholism reduces EGF in the offspring. 相似文献
7.
N Benito A Nú?ez M de Górgolas J Esteban T Calabuig MC Rivas ML Fernández Guerrero 《Canadian Metallurgical Quarterly》1997,157(14):1577-1580
BACKGROUND: Fever is commonly observed in patients with human immunodeficiency virus (HIV) disease and frequently eludes diagnosis. The role of bone marrow biopsy in the diagnosis of fever of unknown origin in patients infected with HIV remains controversial. PATIENTS AND METHODS: One hundred twenty-three consecutive patients with 137 episodes of fever lasting 10 or more days without diagnosis after 1 week of hospitalization were evaluated by bone marrow biopsy. RESULTS: Overall, a specific diagnosis was achieved in 52 episodes by means of culture and histopathological examination (diagnostic yield, 37.9%). Three types of disease were found: mycobacterial infections (n = 36, 69% of documented episodes), including 18 patients with disseminated tuberculosis and 14 with Mycobacterium avium-intracellulare complex infections; non-Hodgkin lymphomas (n = 12, 23%); and visceral leishmaniasis (n = 4, 8%). Although bone marrow cultures were more sensitive than microscopic examination with special stains for the diagnosis of mycobacterial infections, the pathological examination of bone marrow led to a more rapid diagnosis of disease. In addition, the histopathological examination of bone marrow alone led to the diagnosis of a specific condition in 43 episodes (31.3% of all episodes). CONCLUSIONS: Bone marrow biopsy is a useful procedure for the diagnosis of fever in patients with advanced HIV disease, particularly in areas where tuberculosis and leishmaniasis are prevalent. Involvement of the marrow may be the first indication of the existence of extranodal non-Hodgkin lymphoma. For Mycobacterium avium-intracellulare complex infection, blood cultures were more sensitive than bone marrow biopsy. 相似文献
8.
Astaxanthin Extraction From Shrimp Waste by Lactic Fermentation and Enzymatic Hydrolysis of the Carotenoprotein Complex 总被引:1,自引:0,他引:1
ABSTRACT: Natural carotenoids are an alternative to synthetic orange-red pigments. They are present in crustaceans as a protein-pigment complex. In order to extract this highly unstable pigment, crustacean waste must be stabilized; lactic fermentation is a simple and environmentally friendly method to achieve this goal. Shrimp wastes were inoculated with Lactobacillus bacterial cultures. Carotenoids were then extracted with an organic solvent system. Protein-pigment splitting was carried out using a mixture of 4 commercial enzymes; and the protein was separated from the pigment by ultrafiltration. Electrophoretograms showed that the pigment was attached to a 265-kDa protein. Splitting the protein-pigment complex allows studies on pigment absorption, stability and application. 相似文献
9.
Jose R. Rios Viqueira Nikos A. Lorentzos 《The VLDB Journal The International Journal on Very Large Data Bases》2007,16(2):179-200
An SQL extension is formalized for the management of spatio-temporal data, i.e. of spatial data that evolves with respect
to time. The extension is dedicated to applications such as topography, cartography, and cadastral systems, hence it considers
discrete changes both in space and in time. It is based on the rigid formalization of data types and of SQL constructs. Data types are defined in terms of time and
spatial quanta. The SQL constructs are defined in terms of a kernel of few relational algebra operations, composed of the well-known operations of the 1NF model and of two more, Unfold and Fold. In conjunction with previous work, it enables the uniform management of 1NF structures that may contain not only spatio-temporal
but also either purely temporal or purely spatial or conventional data. The syntax and semantics of the extension is fully
consistent with the {SQL:2003} standard. 相似文献
10.
J Einhorn A Guerrero PH Ducrot FD Boyer M Gieselmann W Roelofs 《Canadian Metallurgical Quarterly》1998,95(17):9867-9872
The sex pheromone emitted by the female oleander scale, Aspidiotus nerii (Homoptera, Diaspididae), has been isolated and characterized as (1R, 2S)-cis-2-isopropenyl-1-(4'-methyl-4'-penten-1'-yl)cyclobutaneethanol acetate by using advanced MS and NMR spectroscopic methods, as well as a variety of microderivatization sequences. The structure has been confirmed by stereo- and enantioselective synthesis of the four possible stereoisomers. The absolute configuration has been determined by comparison of the activity of the cis (1S,2R) and (1R, 2S) enantiomers with that exhibited by the natural material in greenhouse bioassays and field tests. The structure of this sesquiterpenoid pheromone is new in the coccids and in the pheromone field in general. 相似文献