Sample size and error in the determination of mode shapes by principal components analysis

Principal components analysis (PCA) is a multivariate statistical technique that transforms a data set having a large number of inter-related variables to a new set of uncorrelated variables called the principal components, determined to allow the dimensionality of the data set to be reduced while retaining as much of the variation present as possible. PCA can be applied to dynamic structural response data to identify the predominant modes of vibration of the structure. Because PCA is a statistical technique, there are errors in the computed modes due to the use of a sample of finite size. The aim of this paper is to study the effect of sample size on the accuracy with which the modes of vibration can be computed. The paper focuses predominantly on elastic response data and examines the potential influence of various parameters such as the period of the structure, the input excitation, and the spatial distribution of mass over the structure. Issues relating to errors in the modes of nonlinear structures are also discussed.     

Monolayer graphene as a saturable absorber in a mode-locked laser

We demonstrate that the intrinsic properties of monolayer graphene allow it to act as a more effective saturable absorber for mode-locking fiber lasers when compared to multilayer graphene. The absorption of monolayer graphene can be saturated at lower excitation intensity compared to multilayer graphene, graphene with wrinkle-like defects, or functionalized graphene. Monolayer graphene has a remarkably large modulation depth of 65.9%, whereas the modulation depth of multilayer graphene is greatly reduced due to nonsaturable absorption and scattering loss. Picosecond ultrafast laser pulses (1.23 ps) can be generated using monolayer graphene as a saturable absorber. Due to the ultrafast relaxation time, larger modulation depth and lower scattering loss of monolayer graphene, it performs better than multilayer graphene in terms of pulse shaping ability, pulse stability, and output energy.      

Biocompatible glutathione capped gold clusters as one- and two-photon excitation fluorescence contrast agents for live cells imaging

The one- and two-photon excitation emission properties of water soluble glutathione monolayer protected gold clusters were investigated. Strong two-photon emission was observed from the gold clusters. The two-photon absorption cross section of these gold clusters in water was deduced from the z-scan measurement to be 189?740 GM, which is much higher compared to organic fluorescent dyes and quantum dots. These gold clusters also showed high photo-stability. The MTT assay showed that these gold clusters have low toxicity even at high concentrations. We have successfully demonstrated their applications for both one and two-photon excitation live cell imaging. The exceptional properties of these gold clusters make them a promising alternative for one- and two-photon bio-imaging and other nonlinear optical applications.     

Using Control Theory to Achieve Service Level Objectives In Performance Management

A widely used approach to achieving service level objectives for a software system (e.g., an email server) is to add a controller that manipulates the target system's tuning parameters. We describe a methodology for designing such controllers for software systems that builds on classical control theory. The classical approach proceeds in two steps: system identification and controller design. In system identification, we construct mathematical models of the target system. Traditionally, this has been based on a first-principles approach, using detailed knowledge of the target system. Such models can be complex and difficult to build, validate, use, and maintain. In our methodology, a statistical (ARMA) model is fit to historical measurements of the target being controlled. These models are easier to obtain and use and allow us to apply control-theoretic design techniques to a larger class of systems. When applied to a Lotus Notes groupware server, we obtain model-fits with R² no lower than 75% and as high as 98%. In controller design, an analysis of the models leads to a controller that will achieve the service level objectives. We report on an analysis of a closed-loop system using an integral control law with Lotus Notes as the target. The objective is to maintain a reference queue length. Using root-locus analysis from control theory, we are able to predict the occurrence (or absence) of controller-induced oscillations in the system's response. Such oscillations are undesirable since they increase variability, thereby resulting in a failure to meet the service level objective. We implement this controller for a real Lotus Notes system, and observe a remarkable correspondence between the behavior of the real system and the predictions of the analysis. This indicates that the control theoretic analysis is sufficient to select controller parameters that meet the desired goals, and the need for simulations is reduced.     

Formation and destruction of H2S in the short residence time pyrolysis of pulverized coal and model compounds

The evolution of sulphur as H₂S from three US bituminous coals, L-cystine, and thianthrene has been studied in the reflected shock region of a chemical shock tube. With heating rates of approximately 3 × 10⁶Ks^?1, to temperatures in the range 1000–2000K, ultimate yields of sulphur from 7 to 70% as H₂S are observed in as little as 1.5 ms. The most important influence on ultimate yields may be the H/S molar ratio in the fuel which corresponds in the hydrocarbons studied with the H₂S yields. Inherent mineral matter may also influence the evolution, as H₂S formation precedes light hydrocarbon formation from the two model compounds, but occurs nearly simultaneously from the coals. The overall rate of H₂S formation for the three coals is adequately described by a reaction, first order with respect to remaining sulphur, with a rate constant $\text{k=8.1 × 10}{}^7\text{exp}\text{(?}\text{15 960}\text{T}\text{) s}{}^{\mathrm{?1}}$. In the pyrolysis of all five solids, the H₂S yields are decreased to below detectable limits at temperatures >1500K. The path for destruction of the H₂S appears to be by reaction with hydrocarbons such as C₂H₂, the concentration of which becomes similar to the H₂S concentration in the temperature range 1500–1600K.     

Encapsulation of Single Plasmonic Nanoparticles within ZIF‐8 and SERS Analysis of the MOF Flexibility

Hybrid nanostructures composed of metal nanoparticles and metal‐organic frameworks (MOFs) have recently received increasing attention toward various applications due to the combination of optical and catalytic properties of nanometals with the large internal surface area, tunable crystal porosity and unique chemical properties of MOFs. Encapsulation of metal nanoparticles of well‐defined shapes into porous MOFs in a core–shell type configuration can thus lead to enhanced stability and selectivity in applications such as sensing or catalysis. In this study, the encapsulation of single noble metal nanoparticles with arbitrary shapes within zeolitic imidazolate‐based metal organic frameworks (ZIF‐8) is demonstrated. The synthetic strategy is based on the enhanced interaction between ZIF‐8 nanocrystals and metal nanoparticle surfaces covered by quaternary ammonium surfactants. High resolution electron microscopy and tomography confirm a complete core–shell morphology. Such a well‐defined morphology allowed us to study the transport of guest molecules through the ZIF‐8 porous shell by means of surface‐enhanced Raman scattering by the metal cores. The results demonstrate that even molecules larger than the ZIF‐8 aperture and pore size may be able to diffuse through the framework and reach the metal core.     

Induction of apoptosis by organotin compounds in vitro: neuronal protection with antisense oligonucleotides directed against stannin

Immortalized cell lines and primary neuronal cultures were used to characterize the selective toxicity of trimethyltin (TMT),triethyltin (TET) and tributyltin (TBT). TBT and TET were cytotoxic at similar concentrations in the immortalized cell lines tested; the 50% toxic concentration (TC50) was 1 to 11 microM. In contrast, immortalized cell lines varied considerably in their sensitivity to TMT, with sensitive cell lines (neuroblastomas, T-, B-cell lines) showing TC50 values of 2 to 8 microM, whereas insensitive cells (NIH-3T3 fibroblast, HTB-14 glioma, TC-7 kidney cells) had TC 50 values > 100 microM. Primary neuronal cell cultures were very sensitive to organotins (TC50 values, 1-10nM), and showed patterns of selective toxicity with respect to neuronal and glial cells. Because organotin toxicity evolves over 24 to 48 hr. we determined whether these compounds induced apoptosis in primary cultures. TMT increased (P < .05) the fraction of apoptotic cells 6 and 12 hr after treatment with TMT at TC50 concentrations. Prior studies suggested that a protein, stannin, was localized in cells sensitive to organotins. Stannin was expressed in several TMT-sensitive cell lines (PC12, T, B cells) and in primary neurons in culture. Stannin was absent in the resistant HTB-14 glioma cell line. The role of stannin in mediating TMT toxicity in primary cultures was investigated by blocking stannin expression with specific antisense oligonucleotides. Treatment of primary cultures with antisense oligonucleotides for 48 hr before and during TMT treatment significantly protected neurons from the neurotoxic and apoptotic effects of TMT. This effect was not observed with scrambled oligonucleotide controls. Thus, TMT may induce apoptosis in sensitive cells, which is partly mediated by stannin. Based on the available data we conclude that stannin expression is necessary, but not sufficient for TMT toxicity.     

Papillary serous carcinoma of the peritoneum in a man: a case report

BACKGROUND: Papillary serous carcinoma of the peritoneum (PSCP) is a rare primary peritoneal tumor, described exclusively in women. It is believed to arise from the secondary müllerian system, which is comprised of the pelvic and lower abdominal mesothelial lining and subjacent (subcoelomic) mesenchyme in women. Both mesotheliomas and PSCP arise from the coelomic epithelium, but are clinicopathologically and biologically distinct entities. METHODS: The authors report clinicopathologic findings in a man, age 74 years, who died 3 months after the diagnosis of an extensive malignant abdominal disease. RESULTS: The routine histologic and immunocytochemical studies of tumor tissue, obtained during the patient's lifetime and at autopsy, validated the unique occurrence of PSCP in a man. CONCLUSIONS: This case illustrates that PSCP can occur in a man and that this diagnosis may be considered in the differential diagnosis of papillary serous tumors of the peritoneum in male patients. Although rare, PSCP is a diagnostically distinct entity the treatment of which is similar to ovarian serous tumors rather than mesotheliomas.