Equilibrium Moisture Characteristics of High Oleic Sunflower Seeds and Kernels

The equilibrium moisture content (EMC) characteristics of high oleic sunflower seeds and kernels between 10 and 55°C were determined by equilibrating the samples to known relative humidities (RH) above saturated salt solutions. EMC of the kernels was lower than that of seeds in the range of 11 to 96% ERH and at 25 and 40°C. Hysteresis effect was found for the EMC properties of seeds and kernels at 25°C. Four EMC-ERH models (modified Henderson, Chung-Pfost, Halsey, and Guggenheim-Anderson-de Boer) and their estimated parameters were evaluated for goodness of fit. GAB and Halsey equations showed the best fitting of experimental data although GAB equation adjusted for temperature described the EMC data the best. Components of the seed as oil content or hull/kernel ratio did not explain the slight differences in EMC found between the varieties.     

A family of converters for UPS production burn-in energy recovery

This paper introduces a family of power converters for power recycling during the burn-in test of synchronized uninterruptible power supplies (UPSs) with sinusoidal output voltage. The use of the power recycler to replace the resistor load bank in the UPSs burn-in test causes great energy savings, and the optimized use of electrical energy contributes in reducing the final cost of the product. The main feature of the new circuits is their ability to draw from the UPS and to inject into the utility-grid currents with low total harmonic distortion (THD) and high power factor (PF). The new circuits operate at constant frequency and are regulated by conventional pulse width modulation (PWM) using dedicated PWM and PF controller integrated circuits developed for power supplies. Circuit operation, mathematical analysis, design example, and experimental results for discontinuous current mode (DCM) and continuous current mode (CCM) operation are provided in this paper     

Hot carrier-induced degradation of gate overlapped lightly dopeddrain (GOLDD) polysilicon TFTs

Hot-carrier injection is known to produce interface states and oxide trapped charge, which, depending upon their spatial distribution, can strongly influence the local electric fields as well as the current flow. In this work, we analyze the hot carrier-induced degradation of gate overlapped lightly doped drain (GOLDD) polysilicon thin film transistors (TFTs) and a new model, which correlates the interface state generation with the hot carrier injection current, is proposed. The defect generation rate has been assumed to depend upon the product of hot electron and hole currents J_eh, and the resulting interface state distribution has been evaluated self-consistently with the current density and carrier concentration distributions. By successive iterations, a complete spatial and time evolution of the interface state distribution has been determined, and the electrical characteristics, calculated with these interface state distributions are in good agreement with the experimental data     

HIF-1α and Pro-Inflammatory Signaling Improves the Immunomodulatory Activity of MSC-Derived Extracellular Vesicles

Despite the strong evidence for the immunomodulatory activity of mesenchymal stromal cells (MSCs), clinical trials have so far failed to clearly show benefit, likely reflecting methodological shortcomings and lack of standardization. MSC-mediated tissue repair is commonly believed to occur in a paracrine manner, and it has been stated that extracellular vesicles (EVs) secreted by MSCs (EVMSC) are able to recapitulate the immunosuppressive properties of parental cells. As a next step, clinical trials to corroborate preclinical studies should be performed. However, effective dose in large mammals, including humans, is quite high and EVs industrial production is hindered by the proliferative senescence that affects MSCs during massive cell expansion. We generated a genetically modified MSC cell line overexpressing hypoxia-inducible factor 1-alpha and telomerase to increase the therapeutic potency of EVMSC and facilitate their large-scale production. We also developed a cytokine-based preconditioning culture medium to prime the immunomodulatory response of secreted EVs (EV_MSC-T-HIF^c). We tested the efficacy of this system in vitro and in a delayed-type hypersensitivity mouse model. MSC-T with an HIF-1α-GFP lentiviral vector (MSC-T-HIF) can be effectively expanded to obtain large amounts of EVs without major changes in cell phenotype and EVs composition. EV_MSC-T-HIF^c suppressed the proliferation of activated T-cells more effectively than did EVs from unmodified MSC in vitro, and significantly blunted the ear-swelling response in vivo by inhibiting cell infiltration and improving tissue integrity. We have developed a long-lived EV source that secretes high quantities of immunosuppressive EVs, facilitating a more standard and cost-effective therapeutic product.     

When a Tritrophic Interaction Goes Wrong to the Third Level: Xanthoxylin From Trees Causes the Honeybee Larval Mortality in Colonies Affected by the River Disease

The “River Disease” (RD), a disorder impacting honeybee colonies located close to waterways with abundant riparian vegetation (including Sebastiania schottiana, Euphorbiaceae), kills newly hatched larvae. Forager bees from RD-affected colonies collect honeydew excretions from Epormenis cestri (Hemiptera: Flatidae), a planthopper feeding on trees of S. schottiana. First-instar honeybee larvae fed with this honeydew died. Thus, we postulated that the nectars of RD-affected colonies had a natural toxin coming from either E. cestri or S. schottiana. An untargeted metabolomics characterization of fresh nectars extracts from colonies with and without RD allowed to pinpoint xanthoxylin as one of the chemicals present in higher amounts in nectar from RD-affected colonies than in nectars from healthy colonies. Besides, xanthoxylin was also found in the aerial parts of S. schottiana and the honeydew excreted by E. cestri feeding on this tree. A larva feeding assay where xanthoxylin-enriched diets were offered to 1^st instar larvae showed that larvae died in the same proportion as larvae did when offered enriched diets with nectars from RD-colonies. These findings demonstrate that a xenobiotic can mimic the RD syndrome in honeybee larvae and provide evidence of an interspecific flow of xanthoxylin among three trophic levels. Further, our results give information that can be considered when implementing measures to control this honeybee disease.

     

Quinacrine noncompetitive inhibitor binding site localized on the Torpedo acetylcholine receptor in the open state

Open-channel blockers of the nicotinic acetylcholine receptor (nAcChR) are widely thought to act sterically by entering and "plugging" the open channel of the nAcChR. However, quinacrine, a fluorescent open-channel blocker, has been recently shown to bind to the nAcChR at a site near the lipid bilayer while the receptor is in a closed, desensitized state, suggesting that at least one open-channel blocker might act allosterically outside the channel [Valenzuela et al. (1992) J. Biol. Chem. 267, 8238]. To determine whether or not quinacrine also binds near the lipid bilayer when the receptor is in an open state, a short-range lipophilic quencher (5-doxylstearate, 5-SA) was used to assess the proximity of the nAcChR-bound quinacrine to the lipid bilayer while the receptor was transiently open by an agonist. Initial experiments using a stopped-flow instrument established the conditions required to monitor a portion of the changes in quinacrine fluorescence associated with its binding to the receptor in the open state. 5-SA (80 microM) reduced the amplitude of the rapid agonist-induced change in quinacrine emission to 44% +/- 12% of the control value, indicating that the quinacrine was binding to a site proximal to the membrane-partitioned 5-SA. Control experiments established that 5-SA had no effect on the ability of the receptor to undergo agonist-induced conformational changes, suggesting that little, if any, 5-SA distributed into the channel lumen and perturbed the functional activity of the receptor. Together, the results indicate that quinacrine binds to a site on the open receptor that is in contact with the lipid bilayer and not in the channel lumen.     

Instructional animations can be superior to statics when learning human motor skills

Based on the assumption of a working memory processor devoted to human movement, cognitive load theory is used to explore some conditions under which animated instructions are hypothesised to be more effective for learning than equivalent static graphics. Using paper-folding tasks dealing with human movement, results from three experiments confirmed our hypothesis, indicating a superiority of animation over static graphics. These results are discussed in terms of a working memory processor that may be facilitated by our mirror-neuron system and may explain why animated instructional animations are superior to static graphics for cognitively based tasks that involve human movement.     

An integration experience of a software architecture and a monitoring infrastructure to deploy applications with non‐functional requirements in computing grids

Resource management is an important aspect to consider regarding applications that might have different non‐functional or operational requirements, when running in distributed and heterogeneous environments. In this context, it is necessary to provide the means to specify the required resource constraints and an infrastructure that can adapt the applications in light of the changes in resource availability. We adopted a contract‐based approach to describe and maintain parallel applications that have non‐functional requirements in a Computing Grid context, called ZeliGrid. To form the supporting infrastructure we have designed a software architecture that integrates some of the Globus services, the LDAP and the NWS monitoring services. Some modules that map the contract approach into software artifacts were also integrated to this architecture. This paper addresses the architecture and integration issues of our approach, as well as how we put the pieces together highlighting deployment and implementation details, which have to consider diverse aspects such as monitoring, security and dynamic reconfiguration. Copyright © 2010 John Wiley & Sons, Ltd.     

Controlled oxidation-reduction potential on dark fermentative hydrogen production from glycerol: Impacts on metabolic pathways and microbial diversity of an acidogenic sludge

The oxidation-reduction potential (ORP) is an important factor in H₂ production via dark fermentation however its effect over microbial diversity in an acidogenic sludge has not, been well studied. This work studies the effect of ORP controlled by hydrogen peroxide and potassium ferricyanide on continuous hydrogen production and microbial diversity in an acidogenic sludge fed (HRT 12 h and pH 5.5) with glycerol. Results show that the more oxidizing ORP control environment (?540 mV) improves H₂ yield by 50–70% (0.31–0.51 molH₂/mol glycerol) over non-ORP control conditions. Oxidizing ORP values were shown to enrich microorganisms of the genus Clostridium, which have been linked to high H₂ yields. Therefore, controlling ORP in an acidogenic sludge was shown to directly modify microbial diversity at the genus level, and could likely to indirectly regulate metabolic function. Additionally, metabolic pathways were regulated by the kind of agent used.