Kommunikationsoberfläche Membran‐ Vorbild für moderne Nanoanalytik

A central event in the life of a cellular system is the interaction between the exterior and the interior compartments. Biochemical signals arrive at the cellular surface, bind to their membrane bound receptor followed by a conformational change triggering the release of an internal chemical or electrical signal.This basic principle is followed by all our perceptive abilities like sense of smell or taste, but also by different signal transduction pathways involved in nerve conductivity, vision, sense of touch or hearing. To follow and mimic this principle of parallel registration is one of the aims of modern nanobiotechnology. If we are able to specifically biofunctionalize small arrays of a solid surface, which could be an electrode or a semiconductor, this approach will enable us to build up devices called “biochips” or “biosensors” that allow the determination of bioactive molecules with high specificity at lowest concentrations. Potential pharmacological active substrates might be screened as well as new receptors may be determined. Applications in genomics as well as proteomics are realistic. The major prerequisite for such a broad spectrum of applications is the fabrication of receptive surfaces. Biomolecules have to be surface‐adsorbed in a highly reproducible, oriented and well organised fashion, a task which in biology is taken by the cellular membranes as external or internal receptive surfaces. The physical principles like hydrogen bonds, electrostatic or hydrophobic interactions that lead to such an organized surface are well known. To synthesize molecular building blocks and to position them onto an otherwise unspecific surface is one of the challenges of nanobiotechnology combining biological knowledge and chemical skills with biophysical techniques that allow to handle or analyze even single molecules.     

Egg Thiamine Status of Lake Ontario Salmonines 1995–2004 with Emphasis on Lake Trout

Alewives (Alosa pseudoharengus), the major prey fish for Lake Ontario, contain thiaminase. They are associated with development of a thiamine deficiency in salmonines which greatly increases the potential for developing an early mortality syndrome (EMS). To assess the possible effects of thiamine deficiency on salmonine reproduction we measured egg thiamine concentrations for five species of Lake Ontario salmonines. From this we estimated the proportion of families susceptible to EMS based on whether they were below the ED20, the egg thiamine concentration associated with 20% mortality due to EMS. The ED20s were 1.52, 2.63, and 2.99 nmol/g egg for Chinook salmon (Oncorhynchus tshawytscha), lake trout (Salvelinus namaycush), and coho salmon (Oncorhynchus kisutch), respectively. Based on the proportion of fish having egg thiamine concentrations falling below the ED20, the risk of developing EMS in Lake Ontario was highest for lake trout, followed by coho (O. kisutch), and Chinook salmon, with the least risk for rainbow trout (O. mykiss). For lake trout from western Lake Ontario, mean egg thiamine concentration showed significant annual variability during 1994 to 2003, when the proportion of lake trout at risk of developing EMS based on ED20 ranged between 77 and 100%. Variation in the annual mean egg thiamine concentration for western Lake Ontario lake trout was positively related (p < 0.001, r² = 0.94) with indices of annual adult alewife biomass. While suggesting the possible involvement of density-dependent changes in alewives, the changes are small relative to egg thiamine concentrations when alewife are not part of the diet and are of insufficient magnitude to allow for natural reproduction by lake trout.     

EXTENSION OF THE RELATIONAL DATABASE AND ITS ALGEBRA WITH ROUGH SET TECHNIQUES

This paper describes a database model based on the original rough sets theory. Its rough relations permit the representation of a rough set of tuples not definable in terms of the elementary classes, except through use of lower and upper approximations. The rough relational database model also incorporates indiscernibility in the representation and in all the operators of the rough relational algebra. This indiscernibility is based strictly on equivalence classes which must be defined for every attribute domain. There are several obvious applications for which the rough relational database model can more accurately model an enterprise than does the standard relational model. These include systems involving ambiguous, imprecise, or uncertain data. Retrieval over mismatched domains caused by the merging of one or more applications can be facilitated by the use of indiscernibility, and naive system users can achieve greater recall with the rough relational database. In addition, applications inherently “rough” could be more easily implemented and maintained in the rough relational database.     

更好的浏览器

就性能而非市场份额来说,Opera一直是MicrosoftInternet Explorer的强有力的替代产品在过去两周,我一直在使用Opera 7.1作为我的主要浏览器。我想说它给我的印象深刻。就性能而非市场份额来说,Opera(www.opera.com) 一直是Microsoft InternetExplorer的强有力的替代产品。我感觉Opera比IE要快,并且它还有一个很棒的下载管理器,在一个单独的屏幕上保留着下载文件。再加上一个弹出式阻截器和带有标签的界面,可以将你打开的页面限定在Opera主窗口内。多元搜索窗口把你的搜索请求发送到Google 和AlltheWeb,然后在并列的窗口显示结果。对O…     

2003年采购指南

现在名牌笔记本电脑也卖到1000美元以下了,你也该想想你的下一台PC是否应该是笔记本     

Using network hacking techniques to disrupt military operational networks

It starts in a small way. An Internet-originated electronic mail message received by various technical ratings and Combat Information Center (CIC) officers on a ship deployed in a very high threat area of the Persian Gulf relays news of a natural disaster involving their families that supposedly occurred during a cookout: