L'observation des processus de groupe: difficultés méthodologiques et perspectives.

Discusses problems involved in collecting data based on observation of small groups. The example of a study of manifestations of cooperation in groups of preschoolers (aged 21–57 mo) is used for illustration. Statistical treatment of observation data and global analysis of data are considered. (English abstract) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Routing and resource optimization in service overlay networks

Service Overlay Networks (SONs) create a virtual topology on top of the Internet and provide end-to-end quality of service guarantees without requiring support by the underlying network.The optimization of the resources utilized by an SON is a fundamental issue for an overlay operator owing to the costs involved and the need to satisfy user requirements. Careful decisions are necessary to provide enough capacity to overlay links, to route traffic, to assign users to access nodes and to deploy overlay nodes.In this paper, we propose two mathematical programming models for the user assignment problem, the traffic routing optimization and the dimensioning of the capacity reserved on overlay links in SONs. The first model minimizes the SON installation cost while providing full access to all users. The second model maximizes the SON profit by selecting which users to serve, based on the expected gain, and taking into consideration budget constraints of the SON operator. Moreover, we extend these models to include the optimization of the number and position of overlay nodes.We provide the optimal solutions of the proposed SON design formulations on a set of realistic-size instances and discuss the effect of different parameters on the characteristics of the planned networks. Numerical results show that the proposed approach is able to solve the problem to the optimum even for large-scale networks.     

Seipin Deficiency as a Model of Severe Adipocyte Dysfunction: Lessons from Rodent Models and Teaching for Human Disease

Obesity prevalence is increasing worldwide, leading to cardiometabolic morbidities. Adipocyte dysfunction, impairing white adipose tissue (WAT) expandability and metabolic flexibility, is central in the development of obesity-related metabolic complications. Rare syndromes of lipodystrophy characterized by an extreme paucity of functional adipose tissue should be considered as primary adipocyte dysfunction diseases. Berardinelli-Seip congenital lipodystrophy (BSCL) is the most severe form with a near absence of WAT associated with cardiometabolic complications such as insulin resistance, liver steatosis, dyslipidemia, and cardiomyopathy. Twenty years ago, mutations in the BSCL2 gene have been identified as the cause of BSCL in human. BSCL2 encodes seipin, an endoplasmic reticulum (ER) anchored protein whose function was unknown back then. Studies of seipin knockout mice or rats demonstrated how seipin deficiency leads to severe lipodystrophy and to cardiometabolic complications. At the cellular levels, seipin is organized in multimers that are particularly enriched at ER/lipid droplet and ER/mitochondria contact sites. Seipin deficiency impairs both adipocyte differentiation and mature adipocyte maintenance. Experiments using adipose tissue transplantation in seipin knockout mice and tissue-specific deletion of seipin have provided a large body of evidence that liver steatosis, cardiomyopathy, and renal injury, classical diabetic complications, are all consequences of lipodystrophy. Rare adipocyte dysfunctions such as in BSCL are the key paradigm to unravel the pathways that control adipocyte homeostasis. The knowledge gathered through the study of these pathologies may bring new strategies to maintain and improve adipose tissue expandability.     

Coordination Environment of Cu(II) Ions Bound to N-Terminal Peptide Fragments of Angiogenin Protein

Angiogenin (Ang) is a potent angiogenic factor, strongly overexpressed in patients affected by different types of cancers. The specific Ang cellular receptors have not been identified, but it is known that Ang–actin interaction induces changes both in the cell cytoskeleton and in the extracellular matrix. Most in vitro studies use the recombinant form (r-Ang) instead of the form that is normally present in vivo (“wild-type”, wt-Ang). The first residue of r-Ang is a methionine, with a free amino group, whereas wt-Ang has a glutamic acid, whose amino group spontaneously cyclizes in the pyro-glutamate form. The Ang biological activity is influenced by copper ions. To elucidate the role of such a free amino group on the protein–copper binding, we scrutinized the copper(II) complexes with the peptide fragments Ang(1–17) and AcAng(1–17), which encompass the sequence 1–17 of angiogenin (QDNSRYTHFLTQHYDAK-NH₂), with free amino and acetylated N-terminus, respectively. Potentiometric, ultraviolet-visible (UV-vis), nuclear magnetic resonance (NMR) and circular dichroism (CD) studies demonstrate that the two peptides show a different metal coordination environment. Confocal microscopy imaging of neuroblastoma cells with the actin staining supports the spectroscopic results, with the finding of different responses in the cytoskeleton organization upon the interaction, in the presence or not of copper ions, with the free amino and the acetylated N-terminus peptides.     

Batch treatment of liquid fraction of pig slurry by intermittent aeration: process simulation and microbial community analysis

BACKGROUND: Manure treatment in intensive livestock farming is required to reduce the risk of negative environmental impacts by nitrogen disposal. Biological removal through intermittent aeration in a single bioreactor is a suitable method for this purpose. The characteristic operation based on the alternation of oxic/anoxic phases confers these systems with certain particularities in terms of process modelling and of understanding the microbial interactions. RESULTS: The performance of a sequencing batch reactor (SBR) treating raw liquid fraction of pig slurry (LFPS) under loading rates of 0.13 g N L^?1 d^?1 was studied. Three different aeration strategies were applied: (1) constant airflow; (2) dissolved oxygen (DO) set‐point; and (3) DO‐based real‐time control. The comparatively low aeration intensity applied throughout the third strategy resulted in the process being performed mainly via nitrite, implying a reduction on the requirements of organic carbon and oxygen. However, a decrease in the nitrification rate was observed under those conditions. Experimental results were satisfactorily simulated by a mathematical model focused on organic carbon and nitrogen removal. Microbial community structure analysis through denaturing gradient gel electrophoretic profiling of 16S rDNA genes showed that the aeration exerted a strong influence on the dominant microbial populations. The presence of the ammonia‐oxidizing species Nitrosomonas europaea, and of denitrifying bacteria related to Thauera sp. and Ralstonia sp., was detected in the strategy at low DO. CONCLUSION: Dependence of model statement and parameter values on the bioreactor operational patterns and piggery wastewater composition was evidenced. Oxygen limitation was responsible for a significant microbial shift in SBR treating LFPS. Copyright © 2009 Society of Chemical Industry     

Object recognition versus object discrimination: Comparison between human infants and infant monkeys.

Human infants (aged 12–32 mo old) and adults learned a delayed nonmatching-to-sample (DNMS) task and single- and multiple-pair discrimination tasks using nonverbal procedures previously used with monkeys. Infants learned discriminations rapidly and at a young age (12 mo), but they required prolonged training and maturation before learning the DNMS task. Adults learned all tasks rapidly. After learning the DNMS task to criterion, memory performance declined systematically in an inverse relation to age. The dissociation in ability of infants on the DNMS vs discrimination tasks closely resembles the dissociation previously reported with infant monkeys (J. Bachevalier & M. Mishkin, 1984). Results from both infant humans and monkeys support a neurocognitive maturational model. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Age and sex differences in the effects of selective temporal lobe lesion on the formation of visual discrimination habits in rhesus monkeys (Macaca mulatta).

Three-month-old infant monkeys with neonatal ablations of either cortical area TE or the amygdala and hippocampus and age-matched normal infants were trained in a concurrent object discrimination task with 24-hr intertrial intervals. Neonatal area TE lesions yielded a transient deficit in visual habit formation, present in the female monkeys only, whereas the same lesions in adult monkeys yielded a severe and long-lasting deficit in both males and females. Although pointing to a greater neural compensation for the early loss as compared with the later loss of cortical area TE, the results also corroborate a recent suggestion (J. Bachevalier et al; see record 1990-03600-001) that, at 3 mo of age, area TE is more fully functional in females than in males. Neither early nor late amygdalohippocampal lesions impaired the ability to form visual discrimination habits, strengthening the proposal that the habit system uses a corticononlimbic circuit. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Bronchodilator response to salbutamol after spontaneous recovery from nonspecific bronchial provocation tests in asthma

Assessment of airway responsiveness by bronchoprovocation and bronchodilatation tests is important in the diagnostic work-up protocol of bronchial asthma and it would be convenient to undertake both tests on the same occasion. However, it is not known whether this can be done accurately. Therefore, this study evaluated the effect of a prior bronchial provocation test on the bronchodilator response to salbutamol after spontaneous recovery of the forced expiratory volume in one second (FEV1) in a group of asthmatic subjects. On two separate occasions at the same time of day, concentration-response studies with inhaled histamine or methacholine, or a sham challenge with normal saline were carried out in a blinded, randomized manner. Changes in airway calibre were followed as FEV1 and agonist responsiveness expressed as the provocative concentration causing a 20% fall in FEV1 (PC20). After either spontaneous recovery or a fixed-duration wait of 45 min (when appropriate), the subjects received 2x100 microg of salbutamol from a metered dose inhaler with a spacer. The bronchodilator response to salbutamol was expressed as a percentage of initial FEV1 (deltaFEV1% init). Bronchial challenge with both agonists failed to alter significantly the airway response to salbutamol, with the deltaFEV1% init mean value (range) being 16.9% (9.0-31.9) and 17.5% (11.6-31.2) on the sham and histamine/methacholine challenge day respectively. It was shown that the degree of bronchodilatation achieved after salbutamol 200 microg is not affected by prior bronchoprovocation testing when enough time is allowed for the airways to recover spontaneously to baseline forced expiratory volume in one second. Thus evaluation of airway responsiveness by both bronchial provocation tests and bronchodilator testing can be assessed reliably within a few hours in asthmatic patients.     

Soft,Implantable Bioelectronic Interfaces for Translational Research

The convergence of materials science, electronics, and biology, namely bioelectronic interfaces, leads novel and precise communication with biological tissue, particularly with the nervous system. However, the translation of lab-based innovation toward clinical use calls for further advances in materials, manufacturing and characterization paradigms, and design rules. Herein, a translational framework engineered to accelerate the deployment of microfabricated interfaces for translational research is proposed and applied to the soft neurotechnology called electronic dura mater, e-dura. Anatomy, implant function, and surgical procedure guide the system design. A high-yield, silicone-on-silicon wafer process is developed to ensure reproducible characteristics of the electrodes. A biomimetic multimodal platform that replicates surgical insertion in an anatomy-based model applies physiological movement, emulates therapeutic use of the electrodes, and enables advanced validation and rapid optimization in vitro of the implants. Functionality of scaled e-dura is confirmed in nonhuman primates, where epidural neuromodulation of the spinal cord activates selective groups of muscles in the upper limbs with unmet precision. Performance stability is controlled over 6 weeks in vivo. The synergistic steps of design, fabrication, and biomimetic in vitro validation and in vivo evaluation in translational animal models are of general applicability and answer needs in multiple bioelectronic designs and medical technologies.     

Quantitative determination of acetaminophen in pharmaceutical formulations using differential scanning calorimetry. Comparison with spectrophotometric method

In this paper our previous researches dealing with compatibility, thermoanalytical characterization, the kinetics of thermal degradation of acetaminophen, either pure or contained in some commercial pharmaceutical formulations, have found applications outlets. In a previous investigation the possible interactions between acetaminophen and four excipients contained in the commercial pharmaceutical formulations were tested. As a continuation of this research in the present study an analytical method based on differential scanning calorimetry (DSC) was applied to determine the acetaminophen content of four commercial pharmaceutical formulations. For a fifth drug it was shown that the method is not applicable owing to observed incompatibility with one of the excipients. Finally, the analytical results obtained were compared with those derived from two UV spectrophotometric methods (one, i.e., “direct method,” recommended by the Pharmacopeia and the other based on the first-order derivative UV spectra).