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排序方式: 共有338条查询结果,搜索用时 15 毫秒
1.
2.
VP Korhonen M Tolvanen JM Hyttinen M Uusi-Oukari R Sinervirta L Alhonen M Jauhiainen OA J?nne J J?nne 《Canadian Metallurgical Quarterly》1997,245(2):482-489
We have generated several transgenic mouse lines and rabbits expressing efficiently (up to 0.3 mg/ml in mice and up to 0.5 mg/ml in rabbits) human erythropoietin in their milk as bovine beta-lactoglobulin fusion protein. Human erythropoietin cDNA was inserted in frame into exon 5 of the bovine beta-lactoglobulin gene with a linker oligonucleotide encoding the cleavage site for bacterial IgA protease. RNA analysis performed on one lactating transgenic mouse and one transgenic rabbit revealed that the fusion gene was expressed almost exlusively in the mammary gland, although low amounts of transgene-derived RNA were detectable in salivary glands and uterus or in the kidney. The fusion protein was specifically cleaved with IgA protease. The erythropoietin part obtained upon digestion had a lower molecular mass than recombinant erythropoietin produced in Chinese hamster ovary cells. By deglycosylation analysis it was shown that the difference in size was due to a different type of glycosylation. Biological activity of the fusion protein, as determined by growth stimulation of TF-1 erythroleukemia cells, was less than 15% of that of human recombinant erythropoietin. Upon digestion of the fusion protein with IgA protease, biological activity comparable to that of the recombinant erythropoietin was recovered. Transgenic males and virgin females did not show signs of enhanced erythropoiesis, but lactating females expressing the transgene displayed transient increases in their hematocrit values. 相似文献
3.
The interaction between negative ions and
3
He superfluids was studied by using a time-of-flight spectrometer having a 10–3 relative resolution. The ions guided through a sample of rotating3He-A were found to be focused into the cores of the vortices present in this rotating superfluid. Two different types, with different core structures and presumably different numbers of circulation quanta associated with them, were found. The ion mobility along the vortex cores,
c
, observed in the absence of external magnetic field, can be explained by the mobility anisotropy of the A phase, which indicates that the vortices are continuous, i.e., consist of the A phase even in the core. In finite magnetic fields, a qualitatively similar continuous vortex structure was found, a result compatible with the earlier nuclear magnetic resonance (NMR) experiments, but also a new vortex type with an anomalously high
c
was seen when the vortex sample was prepared adiabatically. The observed strong ion focusing indicates broken w-symmetry in the core textures. This is in contradiction with the earlier numerical calculations on the optimal continuous vortex texture. Experiments to observe trapping of ions into the vortex cores were performed both in A and B phases. No trapping could be detected. Mobility measurements in stationary liquid were extended down toT=0.3T
c
. Comparison with the calculated transport coefficients yields
A
(T)=1.32
BCS
(T) for the maximal A phase gap atP=29.3 bar, and
B
(T)=1.12
BCS
(T) for the B phase gap at 4.8 bar. In the normal Fermi liquid a shallow minimum of ion mobility was detected aroundT2T
c
=5 mK. 相似文献
4.
Tiina Reponen Anne Hyv rinen Juhani Ruuskanen Taisto Raunemaa Aino Nevalainen 《Journal of aerosol science》1994,25(8):1595-1603
Concentrations and types of airborne fungal spores and their aerodynamic sizes were studied in nine buildings with mould problems and in matched reference buildings in order to characterize the exposure in buildings with mould problems. Samples were taken with Andersen six-stage impactors in the autumn and winter. In winter, total concentrations of fungal spores in buildings with mould problems were twice those in reference buildings, even though all concentrations were quite small. In the autumn, the differences were less obvious due to the masking effect of outdoor air fungi. Also, the compositions of fungal genera differed in buildings with mould problems from that in reference buildings. The difference was most significant in the size range 2.1-4.7 μm. The size distribution of fungal spores in dwellings with mould problems skewed towards larger particles than in reference dwellings. The observed differences in size distribution indicate differences in airborne behaviour of fungal spores and, consequently, in respiratory exposure. 相似文献
5.
Vesa Halava Tero Harju Juhani Karhumki Michel Latteux 《Theoretical computer science》2007,380(3):355-362
In the Post Correspondence Problem (PCP) an instance (h,g) consists of two morphisms h and g, and the problem is to determine whether or not there exists a nonempty word w such that h(w)=g(w). Here we prove that the PCP is decidable for instances with unique blocks using the decidability of the marked PCP. Also, we show that it is decidable whether an instance satisfying the uniqueness condition for continuations has an infinite solution. These results establish a new and larger class of decidable instances of the PCP, including the class of marked instances. 相似文献
6.
Heikki Ailisto Lauri Pohjanheimo Pasi Välkkynen Esko Strömmer Timo Tuomisto Ilkka Korhonen 《Personal and Ubiquitous Computing》2006,10(6):333-344
The prevalent visions of ambient intelligence emphasise natural interaction between user and functions and services embedded in the environment or available through mobile devices. In these scenarios the physical and virtual worlds seamlessly gear into each other, making crossing the border between these worlds natural or even invisible to the user. The bottleneck in reaching these scenarios appear in the natural mapping between the physical objects and their virtual counterparts. The emergence of local connectivity in mobile devices opens possibilities for implementing novel user interface paradigms to enhance this mapping. We present physical selection paradigm for implementing an intuitive human technology interaction for mobile devices. In order to demonstrate the feasibility of the paradigm we implemented two experimental set-ups using commercially available smart phones with IrDA connectivity. The experiments involved selecting a website by physically pointing at its symbol and making a phone call by pointing at an icon representing the person to be called. In tentative user experiments the physical selection method was more time-efficient and it was perceived more positively by the users than a conventional method.
相似文献
Heikki AilistoEmail: |
7.
Harri Tuomisto Petra Lundstrm Ritva Korhonen Jzsef Elter Milan Hladký Jozef Tomek Ludovít Fagula 《Nuclear Engineering and Design》2003,221(1-3):181
There are twelve operating VVER-440/213 reactors in the Czech Republic, Hungary and Slovakia. The plant owners have carried out extensive safety enhancement programmes, which have included measures to improve prevention, mitigation and control of design basis accidents. Recently, there have been a number of projects to study the Severe Accident Management aspects of these plants. The plant owners have also initiated Plant Life Management programmes. The objective of the VERSAFE Concerted Action is to create a network of the VVER-440/213 plant owners and operators, which aims at definition of the further research needs of severe accidents and plant ageing from the utilities’ viewpoint. The starting point is to formulate a general approach to severe accident management and plant life management and then proceed to define the needs for the additional information expected from the safety research. The role of national research institutes and organisations of the partner countries is of crucial importance in performing such research and, thus, creating and maintaining the expertise on the national level. The successful completion of the project will assist in establishing well-defined research projects that are oriented to the needs of the end-users and provide information that can be used to facilitate and support the negotiations of the EU applicant countries operating VVER-440 reactors. 相似文献
8.
9.
Antti Juhani Kaijalainen Pasi Suikkanen L. Pentti Karjalainen John J. Jonas 《Metallurgical and Materials Transactions A》2014,45(3):1273-1283
The effect of austenite pancaking in the non-recrystallization regime on microstructure and texture evolution and thereby on bendability was investigated in an ultrahigh-strength strip steel with a martensitic-bainitic microstructure. The results indicate that an increase in rolling reduction (R tot) below the non-recrystallization temperature, which improves the strength and toughness properties, increases the intensities of the ~{554}〈225〉 α and ~{112}〈110〉 α texture components along the strip centerline and of the ~{112}〈111〉 α component at the surface region. Even with the highest R tot of 79 pct, the bendability along the rolling direction was good, but the preferred alignment of rod-shaped MA constituents along the rolling direction led to a dramatic decrease in the bendability transverse to the rolling direction, with severe cracking occurring even at small bending angles. The early cracking is attributed to localization of the strain in narrow shear bands. It is concluded that the Rtot value has to be limited to guarantee successful bendability. 相似文献
10.