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Ifechukwu E. Adieze Justina C. Orji Rose N. Nwabueze G.O.C. Onyeze 《The International journal of environmental studies》2013,70(3):490-500
Responses to hydrocarbon stress of four tropical plants Panicum maximum, Zea mays, Centrosema sp. and Pueraria sp. grown in crude oil contaminated soils (1%, 5% and 10% w/w) were evaluated in a green house. Plants’ percentage survival, shoot heights, biomass development, and phytotoxicity susceptibility were used as indicators of growth, stress response and hydrocarbon tolerance. Relative to control, shoot heights and biomass of plants reduced with increasing hydrocarbon concentration, but 1% w/w oil-in-soil, stimulated shoot heights (5.9% and 6.4%) and weights (21.9% and 2.3%) in P. maximum and Centrosema sp. respectively. P. maximum tolerated the contaminant stress with biomass yields of 113% and 57% of control respectively in 1% and 10% w/w oil-in-soil. All the plants had 100% survival in 1% w/w, but considerably reduced survival in 10% w/w oil-in-soil. These results show that P. maximum has great potential for phytoremediation of petroleum contaminated soil. 相似文献
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Rokas Lukosevicius Simonas Juzenas Violeta Salteniene Ugne Kulokiene Justina Arstikyte Georg Hemmrich-Stanisak Andre Franke Alexander Link Paulius Ruzgys Saulius Satkauskas Henrikas Pauzas Tadas Latkauskas Gediminas Kiudelis Francesc Balaguer Juozas Kupcinskas Jurgita Skieceviciene 《International journal of molecular sciences》2022,23(4)
Regulatory changes occurring early in colorectal cancer development remain poorly investigated. Since the majority of cases develop from polyps in the adenoma-carcinoma transition, a search of early molecular features, such as aberrations in miRNA expression occurring prior to cancer development, would enable identification of potentially causal, rather than consequential, candidates in the progression of polyp to cancer. In the current study, by employing small RNA-seq profiling of colon biopsy samples, we described differentially expressed miRNAs and their isoforms in the adenoma-carcinoma transition. Analysis of healthy-adenoma-carcinoma sequence in an independent validation group enabled us to identify early deregulated miRNAs including hsa-miR-1246 and hsa-miR-215-5p, the expressions of which are, respectively, gradually increasing and decreasing. Loss-of-function experiments revealed that inhibition of hsa-miR-1246 lead to reduced cell viability, colony formation, and migration rate, thereby indicating an oncogenic effect of this miRNA in vitro. Subsequent western blot and luciferase reporter assay provided evidence of hsa-miR-1246 being involved in the regulation of target AXIN2 and CFTR genes’ expression. To conclude, the present study revealed possible involvement of hsa-miR-1246 in early colorectal cancer development and regulation of tumor suppressors AXIN2 and CFTR. 相似文献
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Justina Mileriene Loreta Serniene Kristina Kondrotiene Lina Lauciene Neringa Kasetiene Dalia Sekmokiene Vaida Andruleviciute Mindaugas Malakauskas 《International Journal of Food Science & Technology》2021,56(6):2853-2863
Consumer interest in a healthy lifestyle and health-promoting natural products is a major driving force for the increasing global demand for bio-functional and sustainable dairy foods. Supplementation of curd cheese with thermo-coagulated acid whey protein (TAWP) led to 8–10% higher contents of moisture, 23–31% of lactose, 12–13% of unsaturated, 5–6% of monounsaturated, 63% of polyunsaturated, and 3–4% of long-chain fatty acids. Lipid quality indices – TI, AI, h/H, and Omega 6/3 among others, were also significantly better than those of control cheese. The addition of indigenous Lactococcus lactis strain enhanced the flavour of cheese samples, decreased the counts of yeast and mould up to 1 log cfu g−1 after 10 days of storage. The replacement of curd with TAWP resulted in novel cheese with overall sensory perception above 77 points and added nutritional and functional value, however, body and texture parameters of modified cheese samples require an improvement. 相似文献
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Elemike Elias E. Onwudiwe Damian C. Mbonu Justina I. 《Journal of Inorganic and Organometallic Polymers and Materials》2021,31(8):3356-3367
Journal of Inorganic and Organometallic Polymers and Materials - Chitosan was isolated from chitin, a direct derivative of snail shell, and further used to form a heterostructure with ZnO... 相似文献
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Jurgis Barkauskas Justina Dak?evi? Remigijus Ju?k?nas Regina Ma?eikien? Gediminas Niaura Gediminas Ra?iukaitis Algirdas Selskis Inga Stankevi?ien? Romualdas Trusovas 《Journal of Materials Science》2012,47(15):5852-5860
Nanocomposite films and coatings were produced from the aqueous solutions containing different proportions of graphite oxide (GO) and Congo red by filtering through a polycarbonate membrane filter into alkaline media. They were examined by electron microscopy, Raman and FTIR spectroscopy, XRD, contact angle, and electrical conductivity measurements. It was established that the Congo red is able to interact through its amino groups with different functional groups of GO to form larger moieties composed of the nanoplatelets of GO. Raman spectroscopy revealed quinoid-like ring structure for dye adhering to the GO. In the case when the interaction occurs with the terminal functional groups located on the edges of the nanoplateletes of GO, larger crystallites in the nanocomposite are formed. The interaction between the Congo red and functional groups of GO situated in a basal plane leads to more compact structure of the nanocomposite. Pulsed laser treatment was used to reduce GO to graphene. Raman spectra of laser treated areas show positive effect of addition of the Congo red on the graphene yield in nanocomposite coatings after the laser treatment. 相似文献
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A multifactor exploration of the photobleaching of Suwannee River dissolved organic matter across the freshwater/saltwater interface 总被引:1,自引:0,他引:1
A four factor central composite experimental design was applied to explore the photobleaching of Suwannee River dissolved organic matter (SRDOM) at 350 nm as a function of the tetravariate system of [SRDOM], total [Fe(III)], [NO3-], and salinity. The ranges of each factor were setto cover their likely concentrations atthe freshwater/ saltwater interface, to encompass the possible conditions encountered during the transition from the terrestrial to marine environment. Each experiment was carried out using a minimum of 25 different initial conditions, with 3-6 replicates/condition. The resulting data set mapped out the effects of multiple photoactive components on the rate of photobleaching. Under the conditions tested (nominally total [Fe(III)] 0.00-4.00 microM; [NO3-] 0.00-60.00 microM; SRDOM 0.00-30.00 mg/L; salinity 0.00-35.00 ppt, polychromatic illumination, pH 8.2) all samples photobleached at all wavelengths measured, and the absorption at 350 nm bleached the most rapidly. The most important factor for predicting the rate of photobleaching at 350 nm was the initial loading of SRDOM; the effect of all other factors on photobleaching was not significant at the 95% level of confidence. Varied salinity, Fe(III), or added D2O had no effect on the rate of photobleaching, indicating that hydroxyl radical, singlet oxygen, and superoxide did not contribute significantly to the loss of the chromophore at 350 nm. The addition of hydroquinone or thiosulfate inhibited photobleaching, suggesting photobleaching may depend on a weaker oxidant such as the excited-state acceptors (derived from SRDOM directly) produced during photoinitiated charge-transfer processes. The advantages of multifactor experimental techniques for exploring SRDOM photochemistry are discussed. 相似文献
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Violeta Gulbiniene Greta Balciuniene Justina Petroniene Rita Viliene Irena Dumalakiene Ingrida Pilypiene Diana Ramasauskaite 《International journal of molecular sciences》2022,23(6)
Preterm premature rupture of membranes (PPROM) interrupts normal lung development, resulting in neonatal respiratory morbidity. Although post-PPROM risks have been researched, only a few studies have investigated noninvasively obtained amniotic fluid (AF) to predict neonatal outcomes. In this study, we aimed to determine whether epidermal growth factor (EGF) in vaginally-collected AF is a significant predictor of neonatal respiratory outcomes after PPROM. We analyzed EGF in vaginally-obtained AF from 145 women with PPROM at 22–34 weeks of gestation. The following neonatal outcomes were included: respiratory distress syndrome, surfactant need, duration and type of respiratory support, and bronchopulmonary dysplasia. We found that EGF concentration was associated with gestational age, and its medians were lower in neonates with respiratory morbidities than unaffected ones. EGF concentrations gradually declined, the lowest being in the most clinically ill patients. EGF < 35 pg/mL significantly predicted the odds of severe respiratory outcomes. EGF in noninvasively collected AF may be a reliable predictor for respiratory outcomes of preterm neonates with PPROM before 34 weeks of gestation. The results of our study may have implications for further research both in noninvasive amniotic fluid analysis and the management of patients after PPROM. 相似文献