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排序方式: 共有63条查询结果,搜索用时 15 毫秒
1.
O Loréal F Levavasseur C Fromaget D Gros A Guillouzo B Clément 《Canadian Metallurgical Quarterly》1993,143(2):538-544
Cellular and molecular mechanisms involved in the deposition of extracellular matrix components in both normal and fibrotic liver are still poorly understood. We have investigated the influence of cooperation between Ito cells and hepatocytes in matrix deposition in vitro. Immunoprecipitation of radiolabeled proteins from media of 5-day-old Ito cell primary cultures showed that these cells secreted high levels of the major basement membrane components, ie, collagen IV, laminin, and entactin/nidogen. By immunocytochemistry, precursors of basement membrane components were found intracellularly, but only scarce deposits were seen around the cells. When hepatocytes were added to 2-day-old Ito cell primary cultures, they established close contacts with Ito cells in less than 24 hours and expressed ZO-1, a tight junction-associated protein not detectable in standard hepatocyte culture. Cytochemistry analysis revealed an abundant extracellular matrix deposited over hepatocyte cords and between hepatocytes and Ito cells. Immunocytochemistry studies showed that this matrix contained laminin, fibronectin, and collagens proIII and IV. These data indicate that a high level of matrix protein synthesis by liver cells in vitro is not sufficient to induce extracellular matrix deposition, and that cell-cell interactions are strongly involved in this process. Hepatocyte/Ito cell co-culture, which may reflect the actual situation in vivo, represents a useful tool for studying liver fibrogenesis. 相似文献
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Daniela López‐Araujo Antonio Loría Arturo Zavala‐Río 《International Journal of Adaptive Control and Signal Processing》2017,31(3):299-313
We solve the simultaneous closed‐loop identification and tracking‐control problems for fully‐actuated Euler–Lagrange systems under input constraints. We use a nonlinear adaptive controller reminiscent of computed‐torque‐type controllers in which linear correction terms are saturated in order to comply with the imposed bounds on the control inputs. Adaptation, reminiscent of gradient methods, is used also with saturation. With respect to related literature, our contribution consists in establishing uniform global asymptotic stability. Therefore, our control scheme ensures robustness with respect to bounded perturbations and uniform convergence of the estimation errors for any initial conditions. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
4.
C. Aguilar-Lugo J. L. Santiago-García M. I. Loría-Bastarrachea D. Guzmán-Lucero L. Alexandrova M. Aguilar-Vega 《Journal of Polymer Research》2016,23(3):49
This work reports two series of structurally different aromatic polyimides based on 4,4´-diaminodiphenylmethane (DPM) and 4,4´-diaminotriphenylmethane (TPM) and three commercial dianhydrides. All TPM-based polyimides formed membranes due to their high molecular weight (inherent viscosities ~0.93–1.14 dl/g), they exhibited high thermal stability (5 %: 490–544 °C), glass transition temperatures between 269 and 293 °C, and reasonable mechanical properties. The incorporation of pendant phenyl moieties in the TPM-based polyimides has a strong effect producing an improvement in solubility, thermal stability, density and gas permeability coefficient in comparison with DPM-based polyimides. The most interesting polyimide TPM-6FDA, containing phenyl and trifluoromethyl as bulky pendant groups, showed higher gas permeability coefficient for CO2 (23.73 Barrer) and the best ideal selectivity to the gas pair CO2/CH4 (α = 28.93). 相似文献
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Abdenour Ait-Ouazzou Susana Lorán Abdelhay Arakrak Amin Laglaoui Carmen Rota Antonio Herrera Rafael Pagán Pilar Conchello 《Food research international (Ottawa, Ont.)》2012,45(1):313-319
The present study evaluates the chemical composition and antimicrobial activity of the essential oils (EOs) of Mentha pulegium L., Juniperus phoenicea L. and Cyperus longus L. from Morocco. The composition of these species was analyzed by GC/MS and 84 components were identified. M. pulegium EO showed a great similarity with EOs coming from other regions, as pulegone, the major component, accounted for about 70% of the EO. The EO of J. phoenicea had as main components α-pinene (24.9%), β-phellandrene (24.4%) and α-terpinyl acetate (12.9%). The EO extracted from C. longus was remarkably rich in sesquiterpene hydrocarbons (83.2%), which included β-himachalene (46.6%), α-humulene (16.9%), and γ-himachalene (10.1%). The antimicrobial activity of these EOs has been evaluated against seven bacteria of significant importance for food hygiene. According to the results, M. pulegium showed the best bacteriostatic and bactericidal effect, followed by J. phoenicea and C. longus. So far as we know, this is the first report on the quantitative composition and biological activity of the essential oil from C. longus. The tested EOs showed a variable degree of antimicrobial activity being M. pulegium the most effective one. 相似文献
7.
A. Gutiérrez-Mejía W. Herrera-Kao S. Duarte-Aranda M.I. Loría-Bastarrachea G. Canché-Escamilla F.J. Moscoso-Sánchez J.V. Cauich-Rodríguez J.M. Cervantes-Uc 《Materials science & engineering. C, Materials for biological applications》2013,33(3):1737-1743
Core–shell nanoparticles consisting of polybutyl acrylate (PBA) rubbery core and a polymethyl methacrylate (PMMA) shell, with different core–shell ratios, were synthesized in order to enhance the fracture toughness of the acrylic bone cements prepared with them. It was observed by TEM and SEM that the core–shell nanoparticles exhibited a spherical morphology with ca. 120 nm in diameter and that both modulus and tensile strength decreased by increasing the PBA content; the desired structuring pattern in the synthesized particles was confirmed by DMA. Also, experimental bone cements were prepared with variable amounts (0, 5, 10 and 20 wt.%) of nanoparticles with a core–shell ratio of 30/70 in order to study the influence of these nanostructured particles on the physicochemical, mechanical and fracture properties of bone cements. It was found that the addition of nanostructured particles to bone cements caused a significant reduction in the peak temperature and setting time while the glass transition temperature (Tg) of cements increased with increasing particles content. On the other hand, modulus and strength of bone cements decreased when particles were incorporated but fracture toughness was increased. 相似文献
8.
A.S. Budiman C.S. Hau-Riege W.C. Baek C. Lor A. Huang H.S. Kim G. Neubauer J. Pak P.R. Besser W.D. Nix 《Journal of Electronic Materials》2010,39(11):2483-2488
While Black’s equation for electromigration (EM) in interconnects with n = 1 is rigorously based on the principles of electrotransport, n > 1 is more commonly observed empirically. This deviation is usually attributed to Joule heating. An alternative explanation
is suggested by the recent discovery of EM plasticity. To examine this possibility, we have retested samples that had been
previously subjected to a predamaging phase of high temperature and current densities to determine whether the loss of median
time to failure (MTF) is retained. We find that the predamaged samples exhibit MTFs that are permanently reduced, which is
a characteristic of EM plasticity. 相似文献
9.
K Loré A S?nnerborg AL Spetz U Andersson J Andersson 《Canadian Metallurgical Quarterly》1998,218(1-2):173-187
We have developed a direct immunocytochemical technique to identify cytokine and chemokine production in epidermal Langerhans cells (LC) and in vitro derived CD14-, CD1a+, CD83+, CD40+ dendritic cells (DC) at the single cell level. Formaldehyde fixation combined with saponin permeabilization preserved cellular morphology and generated a characteristic juxtanuclear staining signal due to the accumulation of cytokine to the Golgi organelle. This approach was used for the assessment of TNF-alpha, IL-6, IL-8, IL-10, IL-12, GM-CSF, MIP-1alpha, MIP-1beta and RANTES producing cells. In contrast, a diffuse cytoplasmic staining was evident for IL-1ra, IL-1alpha and IL-1beta production. IL-1ra and IL-1alpha were expressed in 10-25% of unstimulated cultured cells, while all the other cytokines were undetectable. IL-1ra, IL-1alpha and IL-1beta were also the dominating cytokines, expressed in up to 85% of the DC, after 3 h of LPS stimulation. A significantly lower number of cells (0-5%) synthesized TNF-alpha, IL-6, IL-10, IL-12 and GM-CSF. The incidence of chemokine producing cells (IL-8, RANTES, MIP-1alpha, MIP-1beta) peaked 10 h after LPS stimulation in up to 60% of the DC. Both immature CD83- and mature CD83+ DC as well as LC had a similar cytokine production pattern. Thus, in comparison to monocytes, LPS stimulation of DC generated a lower incidence of TNF-alpha, IL-6, IL-10 and IL-12 producing cells while IL-1 was expressed in a comparable number of cells. 相似文献
10.
Francisco Gallego‐Gómez Eva M. García‐Frutos José M. Villalvilla José A. Quintana Enrique Gutierrez‐Puebla Angeles Monge María A. Díaz‐García Berta Gómez‐Lor 《Advanced functional materials》2011,21(4):738-745
A new carbazole‐related small molecule exhibiting self‐assembly into ordered nanostructures in solution‐processed cast films has been synthesized and its charge‐photogeneration and ‐transport properties have been investigated. Large photoconductivity was measured in the amorphous state while an enormous improvement in the photoconduction properties was observed when the molecules spontaneously organized. Photocurrents increased upon self‐assembly by up to four orders of magnitude, mostly due to the drastic enhancement of the charge photogeneration. A greatly favorable arrangement of the aromatic cores in the resulting nanostructures, which were characterized by X‐ray analysis, may explain these improvements. Photocurrents of mA cm?2, on/off ratios of 104 and quantum efficiencies of unity at low field and light intensity, which are among the best values reported to date, along with the simplicity of fabrication, give this readily‐available organic system great potential for use in plastic optoelectronic devices. 相似文献