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Stefan Harsanyi Zuzana Varchulova Novakova Katarina Bevizova Lubos Danisovic Stanislav Ziaran 《International journal of molecular sciences》2022,23(21)
Bladder cancer (BC) is the 10th most frequent cancer in the world. The initial diagnosis and surveillance of BC require a combination of invasive and non-invasive methods, which are costly and suffer from several limitations. Cystoscopy with urine cytology and histological examination presents the standard diagnostic approach. Various biomarkers (e.g., proteins, genes, and RNAs) have been extensively studied in relation to BC. However, the new trend of liquid biopsy slowly proves to be almost equally effective. Cell-free DNA, non-coding RNA, and other subcellular structures are now being tested for the best predictive and diagnostic value. In this review, we focused on published gene mutations, especially in DNA fragments, but also epigenetic modifications, and non-coding RNA (ncRNA) molecules acquired by liquid biopsy. We performed an online search in PubMed/Medline, Scopus, and Web of Science databases using the terms “bladder cancer”, in combination with “markers” or “biomarkers” published until August 2022. If applicable, we set the sensitivity and specificity threshold to 80%. In the era of precision medicine, the development of complex laboratory techniques fuels the search and development of more sensitive and specific biomarkers for diagnosis, follow-up, and screening of BC. Future efforts will be focused on the validation of their sensitivity, specificity, predictive value, and their utility in everyday clinical practice. 相似文献
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Tomas Selicky Matus Jurcik Barbora Mikolaskova Alexandra Pitelova Nina Mayerova Miroslava Kretova Michaela Osadska Jan Jurcik Roman Holic Lenka Kohutova Jana Bellova Zsigmond Benko Juraj Gregan Silvia Bagelova Polakova Peter Barath Lubos Cipak Ingrid Cipakova 《International journal of molecular sciences》2022,23(21)
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Dag Elgesem Ingo Feinerer Lubos Steskal 《Computer Supported Cooperative Work (CSCW)》2016,25(2-3):167-191
The Snowden affair gave rise to a huge public debate about not only the legitimacy of the secret surveillance programs he revealed but also about Snowden himself and about the accuracy of the information he leaked. In this paper we present an analysis of how the affair was discussed in the English language blogosphere, based on a corpus of 15,000 blog posts written about Snowden and published from June 2013 to June 2014, as a sub-corpus of a larger corpus of 100,000 blog posts on the topic of surveillance, written during the period 2006–2014. Automated tools are used to identify the topics that characterize the blogging about surveillance and the posts about the Snowden affair. Through an in-depth analysis of the blog posts that commented on Snowden’s revelations of the PRISM program for surveillance of social media users, we chart how bloggers responded to Snowden and his role in this disclosure, whether they found the information credible, and the extent to which they expressed criticism of the surveillance practices. The analysis is used as a basis for discussing the role of blogs in the civic engagement during the first phase of the Snowden affair. 相似文献
4.
Frost resistance is one of the most frequent characteristics of concrete. It is a very complex subject and the test methods themselves are still in development and the international consensus on methodology is still sought, too. The determination concrete frost resistance takes several weeks, months or even more than a year. However micro-structural changes as micro-cracks have not been described sufficiently. Acoustic Emission Method as unusual Non-Destructive Methods can help to monitor structural changes during common frost resistance measuring. Note the Acoustic Emission Method detects only active “defects” into monitored structure. Thus when e.g. crack grows some acoustic waves spread from crack place, i. e. from acoustic emission source. The method does not detect geometric discontinuities and “passive” defects. Selected Non-Destructive Methods as Ultrasound, Non-Linear Ultrasonic Spectroscopy, Impact Echo etc. are used to confirm micro-structural changes. The article describes the first experiment with its imperfections, difficulties and possibilities. 相似文献
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Qingpeng Niu James Dinan Sravya Tirukkovalur Anouar Benali Jeongnim Kim Lubos Mitas Lucas Wagner P. Sadayappan 《Concurrency and Computation》2016,28(13):3655-3671
Quantum Monte Carlo (QMC) applications perform simulation with respect to an initial state of the quantum mechanical system, which is often captured by using a cubic B‐spline basis. This representation is stored as a read‐only table of coefficients and accesses to the table are generated at random as part of the Monte Carlo simulation. Current QMC applications, such as QWalk and QMCPACK, replicate this table at every process or node, which limits scalability because increasing the number of processors does not enable larger systems to be run. We present a partitioned global address space approach to transparently managing this data using Global Arrays in a manner that allows the memory of multiple nodes to be aggregated. We develop an automated data management system that significantly reduces communication overheads, enabling new capabilities for QMC codes. Experimental results with QWalk and QMCPACK demonstrate the effectiveness of the data management system. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
7.
Martina Culenova Andreas Nicodemou Zuzana Varchulova Novakova Michaela Debreova Veronika Smolinsk Sona Bernatova Dana Ivanisova Olga Novotna Jaromir Vasicek Ivan Varga Stanislav Ziaran Lubos Danisovic 《International journal of molecular sciences》2021,22(22)
Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive for the donors as well as low cost. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm2, they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two different types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. In several cell cultures, dome-shaped cells were observed as well. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues. 相似文献
8.
Mireia Perera-Gonzalez Attila Kiss Philipp Kaiser Michael Holzweber Felix Nagel Simon Watzinger Eylem Acar Petra Lujza Szabo Inês Fonseca Gonalves Lukas Weber Patrick Michael Pilz Lubos Budinsky Thomas Helbich Bruno Karl Podesser 《International journal of molecular sciences》2021,22(4)
Background: Tenascin-C (TN-C) plays a maladaptive role in left ventricular (LV) hypertrophy following pressure overload. However, the role of TN-C in LV regression following mechanical unloading is unknown. Methods: LV hypertrophy was induced by transverse aortic constriction for 10 weeks followed by debanding for 2 weeks in wild type (Wt) and TN-C knockout (TN-C KO) mice. Cardiac function was assessed by serial magnetic resonance imaging. The expression of fibrotic markers and drivers (angiotensin-converting enzyme-1, ACE-1) was determined in LV tissue as well as human cardiac fibroblasts (HCFs) after TN-C treatment. Results: Chronic pressure overload resulted in a significant decline in cardiac function associated with LV dilation as well as upregulation of TN-C, collagen 1 (Col 1), and ACE-1 in Wt as compared to TN-C KO mice. Reverse remodeling in Wt mice partially improved cardiac function and fibrotic marker expression; however, TN-C protein expression remained unchanged. In HCF, TN-C strongly induced the upregulation of ACE 1 and Col 1. Conclusions: Pressure overload, when lasting long enough to induce HF, has less potential for reverse remodeling in mice. This may be due to significant upregulation of TN-C expression, which stimulates ACE 1, Col 1, and alpha-smooth muscle actin (α-SMA) upregulation in fibroblasts. Consequently, addressing TN-C in LV hypertrophy might open a new window for future therapeutics. 相似文献
9.
Conrad Zimmermann Nico Lubos Cagatay Necati Dagli Prof. Dr.-Ing. Stephan Kabelac 《化学,工程师,技术》2023,95(5):724-731
Eco-friendly mixtures may substitute pure working fluids in thermodynamic cycle processes, yet the existing calculation approaches of mixture condensation are complex due to iterative procedures and multiple input data. To simplify condenser design, this project aims for a new calculation method of mixtures. On this behalf, experimental data for the condensation of ethanol/water and ethanol/octamethyltrisiloxane is provided within a wide composition range to identify the most influential parameters. Finally, a practical prediction method for heat transfer coefficients of mixtures is suggested. 相似文献
10.
Lubos Mitas 《Israel journal of chemistry》2002,42(2-3):261-268
We provide a brief review of recent applications of quantum Monte Carlo (QMC) methods to the electronic structure of nanosystems. We report on calculations of carbon rings with second-order Jahn-Teller effect, energy ordering of silicon clusters, dissociation enthalpies of protonated hydrogen clusters, and other interesting challenges. We point out the QMC accuracy and outline a few ideas that characterize the current position of QMC among the electronic structure methods and its future development. 相似文献