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排序方式: 共有168条查询结果,搜索用时 15 毫秒
1.
A microbubble-powered bioparticle actuator 总被引:4,自引:0,他引:4
Maxwell R.B. Gerhardt A.L. Toner M. Gray M.L. Schmidt M.A. 《Journal of microelectromechanical systems》2003,12(5):630-640
We present the results of a device that uses controllable microbubble actuation to manipulate bioparticles. In order to create a useful device for controlling the position of bioparticles, predictable microfluidic actuation is crucial. The goal of this project was to develop fundamental technology that can be used to manipulate single bioparticles (e.g., cells). We use a thermal bubble actuation method to accomplish this goal. Microbubbles have the advantages of relatively simple electronics and fabrication but can be difficult to control. In this paper, we describe two specific accomplishments: the use of micromachined nucleation cavities to precisely localize thermal bubbles and to achieve controllable bubble formation temperatures and bubble dissipation and the demonstration of controllable microbubbles in a new device for particle sorting. 相似文献
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In this article, we are combining minimization criteria in the colorant separation process for spectral color reproduction. The colorant separation is performed by inverting a spectral printer model: the spectral Yule‐Nielsen modified Neugebauer model. The inversion of the spectral printer model is an optimization operation in which a criterion is minimized at each iteration. The approach we proposed minimizes a criterion defined by the weighted sum of a spectral difference and a perceptual color difference. The weights can be tuned with a parameter α ∞ [0, 1]. Our goal is to decrease the spectral difference between the original data and its reproduction and also to consider perceptual color difference under different illuminant conditions. In order to find the best α value, we initially compare a pure colorimetric criterion and a pure spectral criterion for the reproduction, then we combine them. We perform four colorant separations: the first separation will minimize the 1976 CIELAB color difference where four illuminants are tested, the second separation will minimize an equally weighted summation of 1976 CIELAB color difference with the four illuminants tested independently, the third colorant separation will minimize a spectral difference, and the fourth colorant separation will combine a weighted sum of a spectral difference and one of the two first colorimetric differences previously introduced. This last colorant separation can be tuned with a parameter in order to emphasize on spectral or colorimetric difference. We use a six colorants printer with artificial inks for our experiments. The prints are simulated by the spectral Yule‐Nielsen modified Neugebauer model. Two groups of data are used for our experiments. The first group describes the data printed by our printing system, which is represented by a regular grid in colorant space of the printer and the second group describes the data which is not originally produced by our printing system but mapped to the spectral printer gamut. The Esser test chart and the Macbeth Color Checker test chart have been selected for the second group. Spectral gamut mapping of this data is carried out before performing colorant separation. Our results show improvement for the colorant separations combining a sum of 1976 CIELAB color difference for a set of illuminants and for the colorant separation combining a sum of 1976 CIELAB color difference and spectral difference, especially in the case of spectral data originally produced by the printer. © 2008 Wiley Periodicals, Inc. Col Res Appl, 33, 494–504, 2008 相似文献
4.
U Gerhardt 《Canadian Metallurgical Quarterly》1992,25(4):243-254
The dynamics of aging encompass the topics "aging of society" and "coping with chronic illness". Both are discussed using data from a qualitative study on return to work after coronary artery bypass surgery. Data from the retrospective substudy reveal four forms of aging as biographical development toward early retirement, old-age retirement, or related stages. It emerges that the manner of aging is less influenced by medical rehabilitation or medical history, and more by the patient's occupation and socio-economic status. Case reports are used to show that social class is a facilitation context, but not a determining force, regarding the choice between postoperative retirement or return to work. The final section briefly discusses whether the findings concerning the dynamics of aging of the cohort operated on in the late 1970s apply also to that of the cohort operated on in the late 1980s; this is addressed using some data from the prospective sub-study. 相似文献
5.
de Oliveira Amanda Gerhardt Moreno Janaina Fernandes de Sousa Ana Maria Furtado Esccio Viviane Alves de Oliveira Cavalcanti Guimares Maria Jos da Silva Ana Lucia Nazareth 《Polymer Bulletin》2020,77(6):2889-2904
Polymer Bulletin - Investigating the compatibility mechanism of hybrid composites based on two polymers and one mineral nanofiller is a challenge that needs to be better understood. This study... 相似文献
6.
On Defined Polyethylene Oxide Adducts. V. Nitrogen Containing Polyethylene Oxide Adducts The authors report on the synthesis and properties of longchain α-hydro-ω-N-alkylamino-polyoxyethylenes, there methylethers, and of α-hydro-ω-N-dodecylacetamidopolyoxyethylenes. Special surface properties as solubility, surface activity and micel formation are discussed. 相似文献
7.
Granholm AC Helt C Srivastava N Backman C Gerhardt GA 《Microscopy research and technique》2001,54(5):298-308
Recent studies have suggested that factors in the target tissue influence the degree of plasticity and regeneration following aging and/or specific insults. We have investigated whether young or aged targets differ in their noradrenergic innervation from fetal locus coeruleus (LC) neurons, and also if a specific growth factor, glial cell line-derived neurotrophic factor (GDNF) can affect this innervation pattern. Tissue pieces of fetal brainstem and young (3 months) or old (18 months) iris tissue were transplanted simultaneously into the anterior chamber of the eye of adult hosts. We found that aged iris transplants became innervated to a significantly lesser degree by the cografted LC neurons than young iris transplants. Fetal hippocampal tissue was then grafted to adult hosts, and a fetal brainstem graft containing LC neurons was placed adjacent to the first graft, either at 3 or 21 months post-grafting. Thus, old/young chimeras of the noradrenergic coeruleo-hippocampal pathway were created. Aged hippocampal grafts received a much less dense innervation from co-grafted LC neurons than young hippocampal grafts. Tyrosine hydroxylase-positive-immunoreactive innervation was only found in the outskirts of aged grafts, while the young hippocampal grafts contained an even innervation pattern. The innervation density of hippocampal grafts was significantly enhanced by GDNF treatment. These findings demonstrate that target-derived factors may regulate neuronal plasticity, and that the age of the target is more important for innervation properties than the age of the neuron innervating a particular target. 相似文献
8.
Poly(methyl methacrylate) (PMMA)/indium tin oxide (ITO) nanocomposites were prepared by mechanical mixing and compression molding in order to study the properties and microstructure of the composites. The composites were examined by optical and scanning electron microscopy, impedance spectroscopy, and UV‐VIS spectrophotometry. It was observed that upon compaction of the powders above the glass‐transition temperature of the matrix, the PMMA transforms from spherical to polyhedral‐shaped, and develops sharp edges and flat faces. The ITO nanoparticles do not penetrate the polymer particles, resulting in a novel segregated network microstructure. Excellent correlation between the electrical, optical, and microscopy data also provide good insight about the behavior of the filler as the content is increased in the nanocomposites. There is strong evidence that the ITO nanoparticles are extensively displaced during compaction as the PMMA powders become polyhedral‐shaped. Our results indicate that percolation occurs due to the ITO forming a continuous network along the edges of the faceted PMMA particles. The ITO nanoparticles do not appear on the faces of the PMMA particles until after a percolation path has formed and a marked increase in electrical conductivity has occurred. This behavior significantly diverges from previous models for segregated network microstructures which proposed that percolation occurred as the result of limited displacement of the filler during compaction of the mixed powders. 相似文献
9.
(-)Deprenyl is an irreversible inhibitor of monoamine oxidase B (MAO-B) frequently used as an adjunct therapy in the treatment of Parkinson's Disease. Recent evidence, however, has found that deprenyl's metabolites are associated with an antiapoptotic action within certain neuronal populations. Interestingly, deprenyl's antiapoptotic actions appear not to depend upon the inhibition of MAO-B. Due to a paucity of information surrounding (-)deprenyl's ability to spare neurons in vivo, a series of studies was conducted to further investigate this phenomenon within an apoptotic neuronal death model: kainic acid induced excitotoxicity. Results indicated that (-)deprenyl increased hippocampal neuronal survival compared to saline-matched controls following kainic acid insult. Furthermore, it was discovered that (-)deprenyl treatment could be stopped 14 days following CNS insult by kainate, with evidence of neuronal sparing still present by day 28. In open-field locomotor activity testing of kainate-treated animals, those given subsequent (-)deprenyl treatment showed habituation curves similar to control subjects, while saline-treated animals did not. Given deprenyl's antiapoptotic actions, it is proposed that (-)deprenyl may be beneficial in the treatment of a variety of neurodegenerative diseases where evidence of apoptosis exists, such as Parkinson's and Alzheimer's Disease, by slowing the disease process itself. 相似文献
10.
TM Gerhardt GE Schmahl C Flotho AV Rath CM Niemeyer 《Canadian Metallurgical Quarterly》1997,99(4):882-887
Activation of the Evi-1 gene was first described to be associated with the transformation of murine myeloid leukaemias and has previously been detected in cases of human acute myeloid leukaemia (AML) and chronic myeloid leukaemia (CML) in blast crises and in myelodysplastic syndromes. In this study we determined the frequency and the level of Evi-1 expression in juvenile myelomonocytic leukaemia (JMML) and in normal haemopoiesis. Using RT-PCR and Southern blot hybridization mRNA of Evi-1 could be detected in bone marrow (BM) and peripheral blood (PB) mononuclear cells (MNC) of normal donors. In JMML 12/20 patients examined expressed elevated levels of Evi-1 compared to normal controls. In these samples over-expression of the gene was correlated with a higher percentage of blasts (P = 0.02). Expression levels in BFU-E and CFU-GM derived colonies from BM of JMML patients were lower than those in the corresponding MNC samples. Analysis of CD34+ and CD34- cells demonstrated that Evi-1 is primarily expressed in the CD34+ cell population of both JMML and normal donors. These findings suggest that Evi-1 expression is linked to the early stages of haemopoiesis. Studies on the regulation of Evi-1 expression in CD34+ cells will elucidate its function in progenitor cells and clarify its possible role in the pathogenesis of JMML. 相似文献