The Emi2 Protein of Saccharomyces cerevisiae is a Hexokinase Expressed under Glucose Limitation

Hexokinases catalyze glucose phosphorylation at the first step in glycolysis in eukaryotes. In the budding yeast Saccharomyces cerevisiae , three enzymes for glucose phosphorylation have long been known: Hxk1, Hxk2, and Glk1. In this study, we focus on Emi2, a previously uncharacterized hexokinase-like protein of S. cerevisiae . Our data show that the recombinant Emi2 protein (rEmi2), expressed in Escherichia coli , possesses glucose-phosphorylating activity in the presence of ATP and Mg ²⁺ . It was also found that rEmi2 phosphorylates not only glucose but also fructose, mannose and glucosamine in vitro . In addition, we examined changes in the level of endogenous Emi2 protein in S. cerevisiae in the presence or absence of glucose and a non-fermentable carbon source. We found that the expression of Emi2 protein is tightly suppressed during proliferation in high glucose, while it is strongly upregulated in response to glucose limitation and the presence of a non-fermentable carbon source. Our data suggest that the expression of the endogenous Emi2 protein in S. cerevisiae is regulated under the control of Hxk2 in response to glucose availability in the environment.     

Application of microwaves and millimeter waves for thecharacterization of teeth for dental diagnosis and treatment

This paper presents applications of microwaves and millimeter waves for the characterization of teeth. This is done by measuring the complex permittivity over the frequency range from 0.04 to 40 GHz. These measurements have revealed that dental caries are significantly more lossy to microwaves and millimeter waves than the healthy tooth, and this difference can be used for dental diagnosis. The experimental results have been confirmed by using the finite-difference time-domain (FDTD) method. In addition, millimeter-wave heating of the lossy dental caries can be used as a sterilization treatment. It is concluded that millimeter waves can be used for dental medical diagnosis as well as dental medical treatment     

Prognostic factors in radiation-treated esophageal carcinoma

Prognostic factors in esophageal carcinoma treated with irradiation were examined. The prognosis of 111 patients without metastasis who had received more than 60 Gy was analyzed. Significant associations were found between survival rates and tumor length, stage, radioresponse of the primary tumor and the s.c. X-P classification based on barium contrast radiography; superficial type (tumor limited to the surface of the esophageal wall), tumorous type (solid mass without ulceration), Ul-A type (tumor with shallow ulceration with regular margin), Ul-B type (tumor with deep ulceration or irregular ulcer margin), and funneled type (tumor invading the esophageal wall in a scirrhous pattern). In multiple regression analysis, the X-P classification had the strongest correlation with survival and the survival rates of patients with the superficial type, the tumorous type and the s.c. Ul-A type were significantly higher than those of patients with the other tumor types (p < 0.001).     

Ergonomics solution for crossing collisions based on field assessment of visual environment at urban intersections in Japan

This paper aims to assess quantitatively the actual visual environment of uncontrolled urban downtown intersections in Japan in relation to frequently occurring crossing collisions and to discuss the safety countermeasures for them. In Field Study 1 dealing with direct visibility, our ultra-wide-angle photograph analysis revealed that most of the right/left-ward visible range at 11 intersections were insufficient to check safety, and the quality of direct visibility was closely associated with causing crossing collisions. The countermeasures to reduce a blind area were determined to be a top priority. In Field Study 2 dealing with indirect visibility, more than half of the 25 traffic convex mirrors had marked shortcomings for preventive safety, and ergonomics guidelines ensuring indirect visibility were proposed for installing traffic convex mirrors. Low-cost/low-technology-oriented countermeasures are highly recommended to obtain clear/sufficient images of crucial information satisfying drivers' requirements on traffic convex mirrors in accordance with those ergonomics guidelines was highly recommended. Crossing collisions could be prevented by improvement of poor direct and indirect visibility.     

Physical indices for judging appearance harmony of materials

In visual design, harmony refers to the similarity among objects and the components of these objects that appear as if they belong together. Harmony is related to the body, mind, and emotions as we relate to our living space, which means that the harmony of real objects is an important characteristic. Although the appearance harmony in specific materials has been investigated, harmony among different materials has not received adequate attention. In a previous study, we investigated the appearance harmony among various materials by psychophysical experiments. In this study, we correlated the psychophysical evaluations obtained from the experiments and the physical properties of the materials. By analyzing the relationship between psychophysical evaluations and the physical measurements for each material pairs based on cluster analysis, we found that the property of texture represented by the anisotropy histogram and that of reflectance obtained from the data of the bidirectional reflectance distribution function are important indices that can be used to judge the harmony of materials. In particular, the texture properties were more effective in judging the appearance harmony of material pairs in the fixed condition for illumination and viewing angles, and the reflectance properties were superior for the material pairs in the variable condition for illumination and viewing angles.     

Investigation of metallic color perception using real‐world materials

Metallic colors have a unique appearance of glossiness with features such as highlights, contrast, and reflections on their surface, and therefore, metallic objects are very attractive to humans. Especially, gold, silver, and copper colors are familiar metals used as decorative materials, coins, and other furnishings. However, the mechanism and condition of metallic perception have not been fully investigated. There are a few studies for investigating metallic perception using rendered patches or images, but there is no study using real‐world objects. In our previous study, we developed a simple representation technique that made real objects appear to be made of gold by projecting a solid color onto a target nonmetallic object. By using the representation technique, in this study, we have further investigated the perception of metallic appearance such as gold, silver, and copper using real‐world materials, and analyzed the difference between these metallic perceptions. Our results indicate that the perception of the metallic object is different for gold, silver, and copper. Our new findings are as follows: the glitter required for the perception of gold and silver becomes an obstacle to the perception of copper; the metallic perception reveals that learning experience might be strongly affecting; and luminance adjustment is sensitive to the perception of metallic objects.     

Preparation of ultrafine polyurethane fiber web by laser‐electrospinning combined with air blowing

Thermoplastic polyurethane fiber webs were prepared using a laser‐heated electrospinning process combined with air blowing. The effect of spinning conditions such as air flow rate and air temperature on fiber diameter and molecular weight was investigated. Although the average fiber diameter decreased with increased air flow rate at each air temperature, the diameter increased when the air flow rate was >15 NL min^?1. In addition, the fiber was comparatively thicker with an increase in the air temperature. The variation in the fiber diameter tends to increase with the air flow rate, and a reduction in the molecular weight of the fiber by thermal degradation was suppressed. The thinnest and most uniform fiber with a diameter of 0.9 µm and a diameter coefficient variation of 15% was obtained at an air temperature of 25°C under an air flow rate of 15 NL min^?1. This fiber also had a minimum of decreased molecular weight. POLYM. ENG. SCI., 54:2605–2609, 2014. © 2013 Society of Plastics Engineers