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排序方式: 共有1646条查询结果,搜索用时 15 毫秒
1.
Konstantin V. Dergilev Evgeny K. Shevchenko Zoya I. Tsokolaeva Irina B. Beloglazova Ekaterina S. Zubkova Maria A. Boldyreva Mikhail Yu. Menshikov Elizaveta I. Ratner Dmitry Penkov Yelena V. Parfyonova 《International journal of molecular sciences》2020,21(24)
Cell therapy of the post-infarcted myocardium is still far from clinical use. Poor survival of transplanted cells, insufficient regeneration, and replacement of the damaged tissue limit the potential of currently available cell-based techniques. In this study, we generated a multilayered construct from adipose-derived mesenchymal stromal cells (MSCs) modified to secrete stem cell factor, SCF. In a rat model of myocardium infarction, we show that transplantation of SCF producing cell sheet induced activation of the epicardium and promoted the accumulation of c-kit positive cells in ischemic muscle. Morphometry showed the reduction of infarct size (16%) and a left ventricle expansion index (0.12) in the treatment group compared to controls (24–28%; 0.17–0.32). The ratio of viable myocardium was more than 1.5-fold higher, reaching 49% compared to the control (28%) or unmodified cell sheet group (30%). Finally, by day 30 after myocardium infarction, SCF-producing cell sheet transplantation increased left ventricle ejection fraction from 37% in the control sham-operated group to 53%. Our results suggest that, combining the genetic modification of MSCs and their assembly into a multilayered construct, we can provide prolonged pleiotropic effects to the damaged heart, induce endogenous regenerative processes, and improve cardiac function. 相似文献
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The accuracies of ultrasonography (US) and computed tomography (CT) for determining tumor invasion of the chest wall in lung cancer were compared in a retrospective study of 120 patients. US findings were evaluated preoperatively according to the following criteria: disruption of pleura, extension through the chest wall, and fixation of tumor during breathing. CT findings were evaluated with the following criteria: obtuse angle of the mass to the pleural surface, more than 3 cm contact with the pleural surface, and visible pleural thickening associated with the mass. Chest wall invasion was judged as positive when at least two of the three findings were present with either technique. Nineteen of the 120 patients had chest wall invasion by tumor. The sensitivity of US was 100% and the specificity was 98%. The sensitivity of CT was 68% and the specificity was 66%. The accuracy of US and CT were 98% and 67%, respectively. 相似文献
4.
Mikhail Sorine Isadora van Riemsdijk 《International Journal of Mechanical Sciences》2008,50(9):1411-1422
This article studies tubular hydroforming of high strength low alloy (HSLA) and dual phase (DP600) straight tubes under the action of end feeding loads. Experiments demonstrate that higher end feed loads enhance the formability of the tubes and increase the internal fluid pressure for onset of necking and bursting. Because of the action of the internal pressure and the axial compressive load, the onset of localization (necking) is due to a complex three-dimensional state of stress. Using free expansion experiments, approximate upper and lower bound strain-based forming limit curves are determined for the tube materials. These limit curves, in turn, are used to derive upper and lower bound extended stress-based forming limit curves [Simha et al., Prediction of necking in tubular hydroforming using an extended stress-based FLC. Transactions of the ASME Journal of Engineering Materials and Technology 2007;129(1): 36-47]. In conjunction with finite element computations that use solid elements to model the tube, these stress-based limit curves are used to predict upper and lower bound necking pressures under the action of end feed loading. These predictions of necking pressures, when an appropriate coefficient of tube-die friction is used, are found to bracket the experimentally measured necking pressures. Computations using plane stress shell elements to model the tubes are shown to give erroneous results, since the plane stress approximation is not valid when tubes are hydroformed in a die. 相似文献
5.
SL Romney GY Ho PR Palan J Basu AS Kadish S Klein M Mikhail RJ Hagan CJ Chang RD Burk 《Canadian Metallurgical Quarterly》1997,65(3):483-492
OBJECTIVES: The age-dependence of the development of ventricular arrhythmias was studied in German shepherd dogs with inherited ventricular arrhythmias and sudden death. BACKGROUND: A colony of German shepherd dogs has been established that exhibit inherited ventricular arrhythmias and sudden death. The incidence of arrhythmias increases with age. Because ventricular tachycardia is associated with bradycardia, it was hypothesized that the increased incidence of arrhythmias was related to age-dependent slowing of heart rate. METHODS: Arrhythmia counts and RR intervals were measured from serial ambulatory ECG recordings obtained in 71 dogs (1-48 weeks). In addition, 19 dogs were challenged with phenylephrine (10 micrograms/kg i.v.) at 15, 28, and 45 weeks of age, 10 dogs were challenged with epinephrine (1 microgram/kg i.v.) at 3, 5, 7, 9, 11, 18, and 28 weeks of age, and 10 dogs were challenged at 28 weeks with epinephrine (2.5 micrograms/kg i.v.), before and after propranolol (0.5 mg/kg i.v.). RESULTS: The incidence and severity of ventricular arrhythmias increased between 7 and 28 weeks of age and decreased between 28 and 44 weeks of age. The age-dependent increase in the incidence of ventricular tachycardia was associated with age-dependent reductions in sinus rate. Baroreflex-mediated slowing of the heart rate unmasked arrhythmias in young animals that did not spontaneously display arrthythmias and exacerbated existing arrhythmias in older animals. However, the magnitude of baroreflex-induced bradycardia was similar from 7-18 weeks of age, yet the incidence of arrhythmias increased progressively. Moreover, the waning of ventricular arrhythmias in older animals was not associated with more rapid sinus rates. CONCLUSION: The risk for sudden death in dogs with inherited ventricular arrhythmias increases with age in part because of age-dependent slowing of heart rate and in part because of other heart-rate-independent factors. The correspondence between the development of ventricular tachycardia and sinus pauses is consistent with the hypothesis that ventricular arrhythmias are initiated by early afterdepolarization-induced triggered activity. 相似文献
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A versatile exposure system which can be used to investigate the effects of a 60-Hz alternating magnetic field or traveling magnetic field on small laboratory animals is described. Special attention was given to the design of the electric circuit to simplify the supply requirements and to keep the overall current to a minimum. A magnetic field having a flux density up to 30 mT (300 G) can be generated, and a traveling magnetic field is obtained by reconnecting the coils to a three-phase supply. The total magnetic field due to the currents in the three coils used in the exposure system is analyzed. A computer program was written to evaluate the magnetic field in the region where the animals are located. The difference observed between measured and computed values is attributed to the representation of the multiturn, multilayer coils by equivalent single-turn coils 相似文献
8.
Results from measurements of the electrical resistivity of copper and aluminum conductors under the action of a plane shock wave, and also analysis of the relationship between the electrical resistivity and the hydrostatic pressure and of the theoretical temperature dependence of the electrical resistivity, has made it possible to elucidate the role of defect formation due to the plastic deformation of the metal and to determine its contribution to the increase in electrical resisitivity of the metal. It has been established that the concentration of defects depends on the pressure gradient in a plane shock wave.Institute of Dynamics of Geospheres, Russian Academy of Science, Moscow 117334. Translated from Fizika Goreniya i Vzryva, Vol. 31, No. 3, pp. 47–53, May–June, 1995. 相似文献
9.
K. V. Goncharov 《Glass and Ceramics》2007,64(5-6):187-189
The redox conditions for glass making are investigated on a working glass-melting furnace using molding sand. Data obtained
from instrumental measurements of the ROP of the glass mass and reasons for destabilization of the technological process are
presented.
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Translated from Steklo i Keramika, No. 6, pp. 6–8, June, 2007. 相似文献
10.
Hiroyuki Miki Takanori Takeno Toshiyuki Takagi Alexei Bozhko Mikhail Shupegin Hideya Onodera Takao Komiyama Takashi Aoyama 《Diamond and Related Materials》2006,15(11-12):1898
Superconductivity in a tungsten-containing carbon-oxide film was reported. The film with 500 nm thickness was deposited onto polycrystalline silicon oxides using chemical vapor deposition and the co-sputtering of a tungsten metal target. The bonding state of the carbon atoms and the macroscopic and microscopic crystal structure of the film were investigated by Raman spectroscopy, X-ray diffraction and transmission electron microscopy measurements. From the experimental results, we determined that this film essentially had an amorphous structure. The temperature dependence on resistivity was measured in the temperature range of 2–300 K. Resistive superconducting transition was observed at 3.8 K. The dc magnetizations were measured in the temperature range of 1.8–6.5 K. The diamagnetism resulting from a superconductive state was observed below 3.75 K, which is consistent with a resistive superconducting transition. It is thought that the finite sized clusters of the different superconductive transition temperatures cooperatively produce a macroscopic superconducting phenomenon. 相似文献