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Chen Jyh-Ping Lin Wei-Shin Chang Min-Far 《Journal of the American Oil Chemists' Society》2002,79(3):309-314
Candida cylindracea lipase was entrapped in organic-inorganic hybrid sol-gel polymers made from tetramethoxysilane (TMOS) and alkyltrimethoxysilanes.
By forming the gels within the pores of a nonwoven polyester fabric, a novel immobilized biocatalyst in sheet configuration
based on sol-gel en-trapment of the enzyme was obtained. Lipases immobilized in sol-gel matrices efficiently catalyzed the
direct esterification reaction of geraniol and acetic acid in anhydrous hexane to produce geranyl acetate. The optimal formulation
of the sol-gel solution for enzyme immobilization was at a 20∶1 molar ratio of water to total silane; a 4∶1 molar ratio of
propyltrimethoxysilane to TMOS; hydrolysis time at 30 min; and enzyme loading of 200 mg lipase/g gel. Under these conditions,
protein immobilization efficiency was 91%, and the specific activity of the immobilized enzyme was 2.6 times that of the free
enzyme. Excellent thermal stability was found for the immobilized enzyme in dry form or in hexane solution in the presence
of acetic acid, in which case severe inactivation of free enzyme was observed. The immobilized enzyme retained its activity
after heating at 70°C for 2 h, whereas the free enzyme lost 80% of its activity. 相似文献
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