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1.
S Hakki SJ Harwood MA Morrissey JG Camblin DL Laven WB Webster 《Canadian Metallurgical Quarterly》1997,(335):275-285
When clinical data are insufficient to diagnose infection of bone or joints, nuclear scanning becomes crucial in making an accurate diagnosis. The efficacy of (99m)technetium antigranulocyte monoclonal antibody Fab' fragment (LeukoScan) is prospectively compared with (111)indium white blood cell and (99m)technetium methylene diphosphonate bone scans in 74 patients with suspected musculoskeletal infections. They were grouped according to site of suspected infection: 33 long bones, 23 prosthetic joints, and 18 diabetic feet. Sixty-two of these 74 patients had surgical verification with histopathology or culture. The remaining 12 patients had clinical followup as proof of absence of infection. The overall sensitivity of LeukoScan, (111)indium white blood cell, and (99m)technetium methylene diphosphonate bone scans was 93%, 85% and 92%, respectively. Specificity was 89%, 75% and 52%, and accuracy was 90%, 79% and 74%, respectively. The conclusion from this study is that LeukoScan is more accurate in detecting osteomyelitis, with better sensitivity and specificity in prosthetic joints. Compared with (111)indium white blood cell scans, LeukoScan++ gives superior images, and results are obtained in 1 to 6 hours without biohazard risk from handling blood products. 相似文献
2.
The Yersinia pseudotuberculosis invasin protein is a 986-amino acid protein that promotes bacterial penetration into mammalian cells by avidly binding multiple beta 1-chain integrins. A 192-amino acid carboxyl-terminal domain of invasin was previously shown to be sufficient for binding. Evidence is presented here that a 76-amino acid disulfide loop in the integrin binding domain of invasin is required for invasin-mediated cell binding and entry. Bacterial mutants that were altered at either of 2 cysteine residues in the binding domain of invasin were completely defective for entry. Purified invasin protein derivatives altered at either of these cysteines, in contrast to the wild-type invasin, did not promote either cell binding or penetration. Analysis of proteolytic products of invasin in the presence or absence of reducing agent provided evidence of an intra-chain disulfide bond near the carboxyl terminus of the protein. Alkylation of invasin derivatives with [3H]iodoacetate indicated that these 2 cysteines were normally disulfide-bonded. A treatment that resulted in the maximal reduction of the disulfide bond also resulted in maximal loss of cell attachment activity. These results indicate that the 76-amino acid disulfide loop at the carboxyl terminus of invasin is required for recognition by integrins. 相似文献
3.
KW Scheller J G?rres JG Ross M Wiescher R Harkewicz DJ Morrissey BM Sherrill M Steiner NA Orr JA Winger 《Canadian Metallurgical Quarterly》1994,49(1):46-50
The shortage of suitable liver donors for children has motivated the use of ABO-incompatible (ABO-I) grafts for transplantation in urgent situations. However, survival after ABO-I liver grafts has been reported at about 30% as compared with 80% in cases of ABO-identical or -compatible liver grafts. This difference has been attributed to antibody-mediated, hyperacute or chronic liver rejection, due to preformed ABO antibodies (alloantibodies). In this study, we report our results with ABO-I livers in children without alloantibodies at the time of transplantation. From January 1988 to June 1993, 143 OLT were performed in 122 children. Eight children received 8 ABO-I liver grafts. Of these, 7 patients were included in the study. All 7 were alloantibody free before OLT. Five children were spontaneously alloantibody free, while in 2 children, the plasma alloantibodies were eliminated before and after transplantation using intravenous infusion of specific blood group antigens of the donor blood group (soluble antigens). Immunosuppression consisted of a triple-drug treatment combining CsA, AZA, and steroids. The follow-up period was between 10 and 48 months. One child died from a surgical complication. Six children survived, but 1 died 10 months later from intestinal obstruction. There were no graft losses and no episodes of hyperacute or chronic rejection. The graft and patient survival rate was 71%. There was a 28% incidence of rejection, but all were mild (requiring steroid boluses only). Our results suggest that the absence of ABO alloantibodies at the time of and after transplantation can protect ABO-I liver grafts against antibody-mediated rejection, whether hyperacute or chronic, and that soluble antigens are effective in eliminating alloantibodies in children. 相似文献
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Reinitz Mark Tippens; Morrissey Joseph; Demb Jonathan 《Canadian Metallurgical Quarterly》1994,20(1):161
Ss studied faces in a full- or a divided-attention condition and then received a recognition test that included old faces, new faces constructed by combining facial features from previously studied faces ("conjunction faces"), and partly or completely new faces. Full- but not divided attention Ss responded "old" more often to old than to conjunction faces; all Ss responded "old" to these faces more often than to partially or completely new faces. Thus it is less attentionally demanding to encode facial features than it is to encode their interrelations. Dividing attention had identical effects on an incidental and an intentional learning group. Exp 3 demonstrated that dividing attention primarily affected explicit recollection rather than stimulus familiarity. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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The interfacial properties of proteins extracted from muscle using salt solutions of different concentration and for different extraction times were determined by the drop volume method. At a bulk phase concentration of 10(-2) wt%, it appears that the sarcoplasmic-rich fraction of muscle is more surface active than the salt-soluble fraction. The average equilibrium surface pressure at the air-liquid interface was 21·5 mN m(-1) for an aqueous extract and 20·1 mN m(-1) for a 1m KCl-extract. The equilibrium surface pressure decreased from 21·7 mN m(-1) to 19·50mN m(-1) as the extraction time with Weber-Edsall solution increased from 15 min to 48 h. 相似文献
8.
Hydrous (or ) oxide. films produced on platinum in acid at room temperature usually give rise to two reduction peaks, in addition to the monolayer oxide response, on a negative sweep extending to about 0V. This has been attributed to the presence of two hydrous oxide components (designated as HO1 and HO2) in the film. It was confirmed in the present work that under more severe conditions, 3.0 m H2SO4 at 60°C, an additional, quite significant, peak — apparently due to the presence of a further hydrous oxide component (HO3) — may be observed. The approximate peak maximum potential values (RHE scale) were 0.4 V (HO1), 0.2 V (HO2) and 0.0 V (HO3); the E
p values are given here only as a guide: the processes involved occur under very irreversible conditions and the values vary with factors such as sweep rate, film thickness, temperature, etc. The differences in behaviour between these components is assumed to be due to factors such as the degree of aggregation and compactness in different regions of the gel-type, amorphous deposit. A brief account is given of the increasing evidence from different laboratories of unusual responses for platinum electrodes in aqueous acid solution. 相似文献
9.
Contributions to water retention capacity (% WRC) and texture changes were determined for pork by-products (lung lobes, kidneys), chicken viscera (head, feet and viscera) and mechanically separated chicken (MSC) as affected by pH and various salts in a high-moisture model system. The % WRC for meat by-products and MSC was increased by increased pH (4.5-6.8). Pork lungs and MSC had the highest % WRC (p<0.05) among the meat by-products. Meat by-product % WRC was not signifcantly (p>0.05) affected by salt (2%), phosphate (0.3%) or NaOH (0.075%). Chicken viscera had the lowest (p<0.05) mean texture measurements among the meat by-products and MSC. Strong negative correlations (p<0.05) were obtained for texture with total collagen, soluble collagen and high ionic strength soluble (HIS) proteins. These results should be considered for product quality changes when these by-products are used in formulation of high moisture pet food products. 相似文献
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