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1.
Conventional culture methods have traditionally been considered the "gold standard" for the isolation and identification of foodborne bacterial pathogens. However, culture methods are labor-intensive and time-consuming. A Salmonella enterica serotype Enteritidis-specific real-time PCR assay that recently received interim approval by the National Poultry Improvement Plan for the detection of Salmonella Enteritidis was evaluated against a culture method that had also received interim National Poultry Improvement Plan approval for the analysis of environmental samples from integrated poultry houses. The method was validated with 422 field samples collected by either the boot sock or drag swab method. The samples were cultured by selective enrichment in tetrathionate broth followed by transfer onto a modified semisolid Rappaport-Vassiliadis medium and then plating onto brilliant green with novobiocin and xylose lysine brilliant Tergitol 4 plates. One-milliliter aliquots of the selective enrichment broths from each sample were collected for DNA extraction by the commercial PrepSEQ nucleic acid extraction assay and analysis by the Salmonella Enteritidis-specific real-time PCR assay. The real-time PCR assay detected no significant differences between the boot sock and drag swab samples. In contrast, the culture method detected a significantly higher number of positive samples from boot socks. The diagnostic sensitivity of the real-time PCR assay for the field samples was significantly higher than that of the culture method. The kappa value obtained was 0.46, indicating moderate agreement between the real-time PCR assay and the culture method. In addition, the real-time PCR method had a turnaround time of 2 days compared with 4 to 8 days for the culture method. The higher sensitivity as well as the reduction in time and labor makes this real-time PCR assay an excellent alternative to conventional culture methods for diagnostic purposes, surveillance, and research studies to improve food safety. 相似文献
2.
Synthesis and optical transmission of MgB2 thin films on optically transparent glass are reported. In the 400–1000 nm regime as deposited films show high metallic reflectivity
and very little transmission. After deposition, the films were annealed ex situ and rendered superconducting with T
c of 38 K, approaching that of the bulk material. The reaction conditions where quite soft ∼10 min at 550°C. The optical absorption
coefficient, α and photon energy, E followed a Tauc-type behavior,
= _T (E - E_g )(\alpha E)^{1/2} = \beta _T (E - E_{\rm g} ). The band gap (E
g) was observed to peak at 2.5 eV; but, the slope parameter β
T
behaved monotonically with reaction temperature. Our results indicate that an intermediate semiconducting phase is produced
before the formation of the superconducting phase; also optical measurements provide valuable information in monitoring the
synthesis of MgB2 from its metallic constituents. In addition these films have interesting optical properties that may be integrated into optoelectronics. 相似文献
3.
Sergiu Cecoltan Izabela-Cristina Stancu Diana Maria Drăguşin Andrada Serafim Adriana Lungu Cătălin Ţucureanu Iuliana Caraş Vlad Constantin Tofan Aurora Sălăgeanu Eugeniu Vasile Romain Mallet Daniel Chappard Cristin Coman Mircea Istodorescu Horia Iovu 《Journal of materials science. Materials in medicine》2017,28(10):153
4.
Jiwon Lee Bryan S. Der Christos S. Karamitros Wenzong Li Nicholas M. Marshall Oana I. Lungu Aleksandr E. Miklos Jianqing Xu Tae Hyun Kang Chang-Han Lee Bing Tan Randall A. Hughes Sang Taek Jung Gregory C. Ippolito Jeffrey J. Gray Yan Zhang Brian Kuhlman George Georgiou Andrew D. Ellington 《American Institute of Chemical Engineers》2020,66(3):e16864
We used the molecular modeling program Rosetta to identify clusters of amino acid substitutions in antibody fragments (scFvs and scAbs) that improve global protein stability and resistance to thermal deactivation. Using this methodology, we increased the melting temperature (Tm) and resistance to heat treatment of an antibody fragment that binds to the Clostridium botulinum hemagglutinin protein (anti-HA33). Two designed antibody fragment variants with two amino acid replacement clusters, designed to stabilize local regions, were shown to have both higher Tm compared to the parental scFv and importantly to retain full antigen binding activity after 2 hr of incubation at 70°C. The crystal structure of one thermostabilized scFv variants was solved at 1.6 Å and shown to be in close agreement with the RosettaAntibody model prediction. 相似文献
5.
This paper presents the design of a new reduced order observer to estimate the state for a class of linear time-invariant multivariable systems with unknown inputs. The proposed design approach is a combination of the approaches proposed by Hou and Muller (IEEE Trans. Autom. Control 37:871–875, 1992) and Boubaker (Int. J. Autom. Control Syst. Eng. 5:45–51, 2005); matrix decompositions, state transformations, and substitutions based on coordinate changes are used. It is shown that the problem of reduced order observers for linear systems with unknown inputs can be reduced to a standard one (the unknown input vector will not interfere in the observer equations). The effectiveness of the suggested design algorithm is illustrated by a numerical example (aircraft lateral motion), and, for the same aircraft dynamics, we compare our new observer with other already existing observers from the existence conditions and dynamic characteristics point of view; the superiority of the new designed observer is demonstrated. 相似文献
6.
由于欧洲议会 96/92 EC的指示 ,欧洲正在引入供电竞争。如果供电公司能开发出有区别的电价和服务工作的一揽子规定 (以下按原文直译为费率 -服务包 )奉献给用户群 ,那么电力市场的自由化将使用户得益。新出现和快速变化的营业对供电商代表一种挑战性的新的市场机会。适用于竞争市场条件的新的费率和服务工作的快速设计提出走一条非传统的途径。供电商与用户合作开发的负荷测量工程所产生的实验性的响应费率 ,可能有助于将新的费率 -服务产物迅速推向市场。这些产物包括用于热力储存、自动发电用户所发的电力、实时定价和动态费率以及各种“绿色费率”的创新的费率 -服务包。 相似文献
7.
8.
Summary The axial diffusion of a passive scalar field (e.g. temperature) in Poiseuille flow through a duct is considered, taking account of leakage of heat through the duct boundary. The cases of the two-dimensional channel and the pipe of circular cross-section are considered in detail, and it is shown that (i) the centroid of the scalar field moves (asymptotically) with a velocity intermediate between the mean and the maximum flow rates and increases with increasing wall conductance, and (ii) the effective diffusivity in the flow direction is a decreasing function of wall conductance.The temperature field downstream of a maintained heat source is determined as a function of wall conductance. 相似文献
9.
Dongfang Hu Guodong Han Musango Lungu Zhengliang Huang Zuwei Liao Jingdai Wang Yongrong Yang 《Advanced Powder Technology》2017,28(9):2306-2316
Bubble and particle motion behaviors are investigated experimentally in a gas solid fluidized bed with liquid spray on the side wall. The particles used in the experiment are classified as Geldart B particles. The results reveal that when the fluid drag force is less than the liquid bridge force between particles, liquid distribute all over the bed. Bubble size increases as the increase of inter-particle force, then decreases owing to the increase of particle weight with increasing liquid flow rate. When the fluid drag force is greater than the liquid bridge force, liquid mainly distribute in the upper part of the bed. And it is difficult for the wet particles to form agglomerates. Bubble size decreases with increasing liquid flow rate due to the increasing of minimum fluidization velocity. Besides, the acoustic emission (AE) measurements illustrate that the liquid adhesion and evaporation on particles could enhance the particles motion intensity. Consequently, the bubble and particle behaviors change due to the variation in fluidized gas velocity and liquid flow rate should be seriously considered when attempting to successfully design and operate the side wall liquid spray gas solid fluidized bed. 相似文献
10.
Brindusa Balanuca Raluca Stan Adriana Lungu Eugeniu Vasile 《Designed Monomers and Polymers》2017,20(1):10-17
Lately, renewable resources received great attention in the macromolecular compounds area, regarding the design of the monomers and polymers with different applications. In this study the capacity of several modified vegetable oil-based monomers to build competitive hybrid networks was investigate, taking into account thermal and mechanical behavior of the designed materials. In order to synthesize such competitive nanocomposites, the selected renewable raw material, camelina oil, was employed due to the non-toxicity and biodegradability behavior. General properties of epoxidized camelina oil-based materials were improved by loading of different types of organic-inorganic hybrid compounds – polyhedral oligomeric silsesquioxane (POSS) bearing one (POSS1Ep) or eight (POSS8Ep) epoxy rings on the cages. In order to identify the chemical changes occurring after the thermal curing reactions, FT-IR spectrometry was employed. The new synthesized nanocomposites based on epoxidized camelina oil (ECO) were characterized by dynamic mechanical analyze and thermogravimetric analyze. The morphology of the ECO-based materials was investigate by scanning electron microscopy and supplementary information regarding the presence of the POSS compounds were establish by energy dispersive X-ray analysis and X-ray photoelectron spectroscopy. The smooth materials without any separation phase indicates a well dispersion of the Si–O–Si cages within the organic matrix and the incorporation of this hybrid compounds into the ECO network demonstrates to be a well strategy to improve the thermal and mechanical properties, simultaneously. 相似文献