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排序方式: 共有272条查询结果,搜索用时 15 毫秒
1.
Laura Francesca Pisani Gian Eugenio Tontini Carmine Gentile Beatrice Marinoni Isabella Teani Nicoletta Nandi Pasquale Creo Emanuele Asti Luigi Bonavina Maurizio Vecchi Luca Pastorelli 《International journal of molecular sciences》2021,22(11)
Interleukin (IL)-33 is a member of the interleukin (IL)-1 family of cytokines linked to the development of inflammatory conditions and cancer in the gastrointestinal tract. This study is designed to investigate whether IL-33 has a direct effect on human gastric epithelial cells (GES-1), the human gastric adenocarcinoma cell line (AGS), and the gastric carcinoma cell line (NCI-N87) by assessing its role in the regulation of cell proliferation, migration, cell cycle, and apoptosis. Cell cycle regulation was also determined in ex vivo gastric cancer samples obtained during endoscopy and surgical procedures. Cell lines and tissue samples underwent stimulation with rhIL-33. Proliferation was assessed by XTT and CFSE assays, migration by wound healing assay, and apoptosis by caspase 3/7 activity assay and annexin V assay. Cell cycle was analyzed by means of propidium iodine assay, and gene expression regulation was assessed by RT-PCR profiling. We found that IL-33 has an antiproliferative and proapoptotic effect on cancer cell lines, and it can stimulate proliferation and reduce apoptosis in normal epithelial cell lines. These effects were also confirmed by the analysis of cell cycle gene expression, which showed a reduced expression of pro-proliferative genes in cancer cells, particularly in genes involved in G0/G1 and G2/M checkpoints. These results were confirmed by gene expression analysis on bioptic and surgical specimens. The aforementioned results indicate that IL-33 may be involved in cell proliferation in an environment- and cell-type-dependent manner. 相似文献
2.
A digital imaging approach was applied to investigate mortar morphology in thin sections; in particular, the binder/aggregate ratio and the grading curve of five mortar bars were attained by digital image processing (DIP), using the Image Pro Plus 4.1 software package. The imaging procedure employed image segmentation, to extract mortar aggregate, and image filtering, to fix grain boundaries. The results show that digital image processing may be considered as an alternative method to mechanical sieving for the characterisation of mortar morphology, as it appears to be quicker and more accurate than the traditional method. However, digital image processing exhibits limits, which are discussed in the text. 相似文献
3.
Gianni S Calosci N Aelen JM Vuister GW Brunori M Travaglini-Allocatelli C 《Protein engineering, design & selection : PEDS》2005,18(8):389-395
PDZ domains represent a large family of protein-interaction modules associated with a variety of unrelated proteins with different functions. We report a complete characterization of the kinetic folding mechanism of a fluorescent variant of PDZ2 from PTP-BL, investigated under a variety of different experimental conditions. For this purpose, we engineered a fluorescent variant of this protein Y43W (called pseudo-wild-type, pWT43). The results suggest the presence of a high-energy intermediate in the folding of PDZ2, as revealed by a pronounced non-linear dependence of the unfolding rate constant on denaturant concentration. Such an intermediate may or may not be detectable depending on the experimental conditions, giving rise to apparent two-state folding under stabilizing conditions (e.g. in the presence of sodium sulfate). Interestingly, even under these conditions, three-state folding can be restored by selectively destabilizing the native-like rate-limiting barrier by one specific mutation (V44A). Finally, we show that data taken on pWT43 under different experimental conditions (e.g. different pH values from 2.1 to 8.0 or in the presence of a stabilizing salt) and also data on a site-directed conservative mutant can be rationalized in terms of a simple reaction scheme involving a single set of intermediates and transition states. 相似文献
4.
Artificial institutions: a model of institutional reality for open multiagent systems 总被引:2,自引:0,他引:2
Nicoletta Fornara Francesco Viganò Mario Verdicchio Marco Colombetti 《Artificial Intelligence and Law》2008,16(1):89-105
Software agents’ ability to interact within different open systems, designed by different groups, presupposes an agreement
on an unambiguous definition of a set of concepts, used to describe the context of the interaction and the communication language
the agents can use. Agents’ interactions ought to allow for reliable expectations on the possible evolution of the system;
however, in open systems interacting agents may not conform to predefined specifications. A possible solution is to define
interaction environments including a normative component, with suitable rules to regulate the behaviour of agents. To tackle
this problem we propose an application-independent metamodel of artificial institutions that can be used to define open multiagent
systems. In our view an artificial institution is made up by an ontology that models the social context of the interaction,
a set of authorizations to act on the institutional context, a set of linguistic conventions for the performance of institutional
actions and a system of norms that are necessary to constrain the agents’ actions. 相似文献
5.
6.
Depalo Nicoletta Iacobazzi Rosa Maria Valente Gianpiero Arduino Ilaria Villa Silvia Canepa Fabio Laquintana Valentino Fanizza Elisabetta Striccoli Marinella Cutrignelli Annalisa Lopedota Angela Porcelli Letizia Azzariti Amalia Franco Massimo Curri Maria Lucia Denora Nunzio 《Nano Research》2017,10(7):2431-2448
Currently,sorafenib is the only systemic therapy capable of increasing overall survival of hepatocellular carcinoma patients.Unfortunately,its side effects,particularly its overall toxicity,limit the therapeutic response that can be achieved.Superparamagnetic iron oxide nanoparticles (SPIONs) are very attractive for drug delivery because they can be targeted to specific sites in the body through application of a magnetic field,thus improving intratumoral accumulation and reducing adverse effects.Here,nanoformulations based on polyethylene glycol modified phospholipid micelles,loaded with both SPIONs and sorafenib,were successfully prepared and thoroughly investigated by complementary techniques.This nanovector system provided effective drug delivery,had an average hydrodynamic diameter of about 125 nm,had good stability in aqueous medium,and allowed controlled drug loading.Magnetic analysis allowed accurate determination of the amount of SPIONs embedded in each micelle.An in vitro system was designed to test whether the SPION micelles can be efficiently held using a magnetic field under typical flow conditions found in the human liver.Human hepatocellular carcinoma (HepG2) cells were selected as an in vitro system to evaluate tumor cell targeting efficacy of the superparamagnetic micelles loaded with sorafenib.These experiments demonstrated that this delivery platform is able to enhance sorafenib's antitumor effectiveness by magnetic targeting.The magnetic nanovectors described here represent promising candidates for targeting specific hepatic tumor sites,where selective release of sorafenib can improve its efficacy and safety profile. 相似文献
7.
Some applications require autonomous robots to search an initially unknown environment for static targets, without any a priori information about environment structure and target locations. Targets can be human victims in search and rescue or materials
in foraging. In these scenarios, the environment is incrementally discovered by the robots exploiting exploration strategies
to move around in an autonomous and effective way. Most of the strategies proposed in literature are based on the idea of
evaluating a number of candidate locations on the frontier between the known and the unknown portions of the environment according
to ad hoc utility functions that combine different criteria. In this paper, we show some of the advantages of using a more theoretically-grounded
approach, based on Multi-Criteria Decision Making (MCDM), to define exploration strategies for robots employed in search and
rescue applications. We implemented some MCDM-based exploration strategies within an existing robot controller and we evaluated
their performance in a simulated environment. 相似文献
8.
9.
Detection of epileptic electroencephalogram based on Permutation Entropy and Support Vector Machines 总被引:2,自引:0,他引:2
The electroencephalogram (EEG) has proven a valuable tool in the study and detection of epilepsy. This paper investigates for the first time the use of Permutation Entropy (PE) as a feature for automated epileptic seizure detection. A Support Vector Machine (SVM) is used to classify segments of normal and epileptic EEG based on PE values. The proposed system utilizes the fact that the EEG during epileptic seizures is characterized by lower PE than normal EEG. It is shown that average sensitivity of 94.38% and average specificity of 93.23% is obtained by using PE as a feature to characterize epileptic and seizure-free EEG, while 100% sensitivity and specificity were also obtained in single-trial classifications. 相似文献
10.
Applying EuroWordNet to Cross-Language Text Retrieval 总被引:1,自引:0,他引:1
Julio Gonzalo Felisa Verdejo Carol Peters Nicoletta Calzolari 《Computers and the Humanities》1998,32(2-3):185-207
We discuss ways in which EuroWordNet (EWN) can be used in multilingual information retrieval activities, focusing on two approaches to Cross-Language Text Retrieval that use the EWN database as a large-scale multilingual semantic resource. The first approach indexes documents and queries in terms of the EuroWordNet Inter-Lingual-Index, thus turning term weighting and query/document matching into language-independent tasks. The second describes how the information in the EWN database could be integrated with a corpus-based technique, thus allowing retrieval of domain-specific terms that may not be present in our multilingual database. Our objective is to show the potential of EuroWordNet as a promising alternative to existing approaches to Cross-Language Text Retrieval. 相似文献