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Anodar Charuchinda Robert Molloy Jintana Siripitayananon Nipapan Molloy Montira Sriyai 《Polymer International》2003,52(7):1175-1181
Some of the main factors affecting the small‐scale melt spinning of poly(ε‐caprolactone), PCL, monofilament fibres have been studied. These factors included spinning temperature, extrusion rate, take‐up rate and draw ratio. The underlying influence of the polymer's own characteristic properties, in particular its chemical structure, transition temperatures (Tg, Tm) and crystallizability, were also interpreted within the context of the melt spinning process. Physically, the as‐spun fibres obtained were uniform in diameter and smooth in surface appearance. They were also semi‐crystalline (>50%) in morphology. Mechanically, however, they were still very weak and highly extensible. Subsequent off‐line cold‐drawing at room temperature introduced the required degree of molecular orientation to reinforce the fibres, yielding tensile strengths of approaching 300 MPa. PCL fibres of precisely controlled physical dimensions and matrix morphology are attracting increasing interest for use in biomedical applications. This paper describes how this control can be achieved through the processing operation. Copyright © 2003 Society of Chemical Industry 相似文献
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Mangkorn Srisa‐ard Robert Molloy Nipapan Molloy Jintana Siripitayananon Montira Sriyai 《Polymer International》2001,50(8):891-896
A random terpolymer of L ‐lactide (LL), ?‐caprolactone (CL) and glycolide (G) has been synthesized in bulk at 130 °C using stannous octoate as the coordination–insertion initiator. The terpolymer, poly(LL‐ran‐CL‐ran‐G), has been characterized by a combination of analytical techniques: GPC, 1H NMR, 13C NMR, DSC and TG. Molecular weight characterization by GPC shows a unimodal molecular weight distribution with values of M n = 1.01 × 105 g mol?1 and M w / M n = 2.17. Compositional and microstructural analysis by 1H NMR and 13C NMR, respectively, reveal a terpolymer composition of LL:CL:G = 74:15:11 (mol%) with a chain microstructure consistent with random monomer sequencing. This latter view is supported by the terpolymer temperature transitions (Tg and Tm) from DSC and the thermal decomposition profile from TG. The results and, in particular, the conclusion that it is a random rather than a statistical terpolymer are discussed in the light of current theories regarding the mechanism of this type of polymerization. © 2001 Society of Chemical Industry 相似文献
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Jiraporn Kantapan Nampeung Anukul Nipapan Leetrakool Gwenaël Rolin Jackie Vergote Nathupakorn Dechsupa 《International journal of molecular sciences》2021,22(16)
Cell-based therapy is a highly promising treatment paradigm in ischemic disease due to its ability to repair tissue when implanted into a damaged site. These therapeutic effects involve a strong paracrine component resulting from the high levels of bioactive molecules secreted in response to the local microenvironment. Therefore, the secreted therapeutic can be modulated by preconditioning the cells during in vitro culturing. Herein, we investigated the potential use of magnetic resonance imaging (MRI) probes, the “iron–quercetin complex” or IronQ, for preconditioning peripheral blood mononuclear cells (PBMCs) to expand proangiogenic cells and enhance their secreted therapeutic factors. PBMCs obtained from healthy donor blood were cultured in the presence of the iron–quercetin complex. Differentiated preconditioning PBMCs were characterized by immunostaining. An enzyme-linked immunosorbent assay was carried out to describe the secreted cytokines. In vitro migration and tubular formation using human umbilical vein endothelial cells (HUVECs) were completed to investigate the proangiogenic efficacy. IronQ significantly increased mononuclear progenitor cell proliferation and differentiation into spindle-shape-like cells, expressing both hematopoietic and stromal cell markers. The expansion increased the number of colony-forming units (CFU-Hill). The conditioned medium obtained from IronQ-treated PBMCs contained high levels of interleukin 8 (IL-8), IL-10, urokinase-type-plasminogen-activator (uPA), matrix metalloproteinases-9 (MMP-9), and tumor necrosis factor-alpha (TNF-α), as well as augmented migration and capillary network formation of HUVECs and fibroblast cells, in vitro. Our study demonstrated that the IronQ-preconditioning PBMC protocol could enhance the angiogenic and reparative potential of non-mobilized PBMCs. This protocol might be used as an adjunctive strategy to improve the efficacy of cell therapy when using PBMCs for ischemic diseases and chronic wounds. However, in vivo assessment is required for further validation. 相似文献
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Microchip capillary electrophoresis(MCE) has significant impact on diagnostic testing.One of the most importance in clinical analysis test is the determination of cholesterol level in blood,because its increase is associated with coronary heart disease which is a major cause of death world over.Poly-dimethylsiloxane(PDMS) was material used to make a MCE for determination of cholesterol.Hydrogen peroxide was generated from the oxidation of cholesterol with cholesterol oxidase(ChOx) and was detected electroch... 相似文献
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