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1.
Male Wistar rats were fed for 4 wk on diets containing 2% oxidized corn oil. Liver tissue was then studied to determine the
effect of feeding peroxidized oil on lipogenic enzymes. Although substances which reacted with thiobarbituric acid increased
in liver microsomes and mitochondria with increasing peroxide values of the dietary corn oil fed, the activities of glucose-6-phosphate
dehydrogenase, malic enzyme and acetyl-CoA carboxylase in liver were unchanged. However, when rats were fed for 2 wk on diets
containing 10% fat, of which 0.5, 5 or 10% was unoxidized corn oil and the remainder was hydrogenated beef tallow filler,
the lipogenic enzyme activities and also the liver triglyceride levels were observed to decrease with increasing amounts of
dietary corn oil. Therefore, although a synthetic diet containing corn oil was easy to oxidize spontaneously, the reductions
of lipogenic enzymes in rats fed the diet would not have been caused by lipid peroxides but by unsaturated fatty acids themselves. 相似文献
2.
The liver lipogenic enzymes are compared among rats, chickens, frogs and fish. Although the apparent Km values of glucose-6-phosphate
dehydrogenase for glucose-6-phosphate are not much different among all the species, those of malic enzyme for malate are much
higher in chickens and fish than in rats and frogs. Glucose-6-phosphate dehydrogenase showed very high activities compared
with malic enzyme in fish liver, and malic enzyme showed high activities in chicken liver. Although the apparent Km values
of acetyl-CoA carboxylase and fatty acid synthetase for substrates are in the same range among all the animals, the activity
of acetyl-CoA carboxylase seems to be extremely low in fish and frog livers, and that of fatty acid synthetase is low in frog
livers only. In addition, the apparent Km values of α-glycerophosphate acyltransferase of fish liver are very high, and the
enzyme activity appears to be extremely low compared to the others. Therefore, the enzymes at the first steps of both fatty
acid and glycerolipid syntheses of poikilothermos animals appear to be very low. On the other hand, the Ouchterlony double-diffusion
patterns showed that the lipogenic enzymes of chickens, frogs and fish are immunologically different from those of rats, with
the exception of acetyl-CoA carboxylase in chickens. Therefore, it is suggested that the fatty acid and glycerolipid forming
systems of poikilothermos animals are quite different from those of homoiothermos and the lipogenesis is very low in poikilothermos. 相似文献
3.
Yasuro Ikuma Eriko Shimada Nobuko Okamura 《Journal of the American Ceramic Society》2005,88(2):419-423
An extensive X-ray study of CeO2 –Nd2 O3 solid solutions was performed, and the densities of solid solutions containing various concentrations of NdO1.5 were measured using several techniques. Solid solutions containing 0–80 mol% NdO1.5 were synthesized by coprecipitation from Ce(NO3 )3 and Nd(NO3 )3 aqueous solutions, and the coprecipitated samples were sintered at 1400°C. A fluorite structure was observed for CeO2 –NdO1.5 solid solutions with 0–40 mol% NdO1.5 , which changed to a rare earth C-type structure at 45–75 mol% NdO1.5 . The change in the lattice parameters of CeO2 –NdO1.5 solid solutions, when plotted with respect to the NdO1.5 concentration, showed that the lattice parameters followed Vegard's law in both the fluorite and rare earth C-type regions. The maximum solubility limit for NdO1.5 in CeO2 solid solution was approximately 75 mol%. The relationship between the density and the Nd concentration indicated that the defect structure followed the anion vacancy model over the entire range (0–70 mol% NdO1.5 ) of solid solution. 相似文献
4.
This paper presents a new notion of coprimeness over multivariable polynomial matrices, where a single variable is given priority over the remaining variables. From a characterization through a set of common zeros of the minors, it is clarified that the presented coprimeness is equivalent to weakly zero coprimeness in the particular variable. An application of the presented coprimeness to control systems with non-commensurate delays and finite spectrum assignment is also presented. Because the presented coprimeness is stronger than minor coprimeness, non-commensurate delays are difficult to deal with in algebraic control theory. The “rational ratio condition” between delays, which can reduce non-commensurate delays to commensurate delays, proves to be both powerful and practical concept in algebraic control theory for delay systems. 相似文献
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Kazuki Hirose Miho Kuwahara Eiji Nakata Tomonori Tetsunaga Kazuki Yamada Kenta Saiga Masaharu Takigawa Toshifumi Ozaki Satoshi Kubota Takako Hattori 《International journal of molecular sciences》2022,23(23)
Osteoarthritis (OA) occurs not only in the knee but also in peripheral joints throughout the whole body. Previously, we have shown that the expression of cellular communication network factor 3 (CCN3), a matricellular protein, increases with age in knee articular cartilage, and the misexpression of CCN3 in cartilage induces senescence-associated secretory phenotype (SASP) factors, indicating that CCN3 promotes cartilage senescence. Here, we investigated the correlation between CCN3 expression and OA degenerative changes, principally in human femoral head cartilage. Human femoral heads obtained from patients who received total hip arthroplasty were categorized into OA and femoral neck fracture (normal) groups without significant age differences. Gene expression analysis of RNA obtained from femoral head cartilage revealed that CCN3 and MMP-13 expression in the non-weight-bearing part was significantly higher in the OA group than in the normal group, whereas the weight-bearing OA parts and normal cartilage showed no significant differences in the expression of these genes. The expression of COL10A1, however, was significantly higher in weight-bearing OA parts compared with normal weight-bearing parts, and was also higher in weight-bearing parts compared with non-weight-bearing parts in the OA group. In contrast, OA primary chondrocytes from weight-bearing parts showed higher expression of CCN3, p16, ADAMTS4, and IL-1β than chondrocytes from the corresponding normal group, and higher ADAMTS4 and IL-1β in the non-weight-bearing part compared with the corresponding normal group. Acan expression was significantly lower in the non-weight-bearing group in OA primary chondrocytes than in the corresponding normal chondrocytes. The expression level of CCN3 did not show significant differences between the weight-bearing part and non-weight-bearing part in both OA and normal primary chondrocytes. Immunohistochemical analysis showed accumulated CCN3 and aggrecan neoepitope staining in both the weight-bearing part and non-weight-bearing part in the OA group compared with the normal group. The CCN3 expression level in cartilage had a positive correlation with the Mankin score. X-ray analysis of cartilage-specific CCN3 overexpression mice (Tg) revealed deformation of the femoral and humeral head in the early stage, and immunohistochemical analysis showed accumulated aggrecan neoepitope staining as well as CCN3 staining and the roughening of the joint surface in Tg femoral and humeral heads. Primary chondrocytes from the Tg femoral head showed enhanced expression of Ccn3, Adamts5, p16, Il-6, and Tnfα, and decreased expression of Col2a1 and -an. These findings indicate a correlation between OA degenerative changes and the expression of CCN3, irrespective of age and mechanical loading. Furthermore, the Mankin score indicates that the expression level of Ccn3 correlates with the progression of OA. 相似文献
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8.
Masayuki Morita Ryo Arizono Nobuko Yoshimoto Minato Egashira 《Journal of Applied Electrochemistry》2014,44(4):447-453
The electrochemical activation process of the so-called “alkali-treated soft carbon” (ASC) has been examined in organic electrolyte solutions. SEM observation demonstrated that the edge plane of graphene structure of the ASC particle becomes rough after the activation, and XRD measurements indicated that the average lattice constant of graphene stacking in ASC increases after the activation process. Ex-situ 7Li NMR measurements proved that the insertion of cation (Li+) into the pore structure of ASC is associated with the activation process in the electrolyte dissolving Li salt. The pore-size distribution determined from N2-gas adsorption for ASC electrodes before and after the electrochemical activation indicates that the pore structure becomes developed after the electrochemical polarization, especially in the pore-diameter range of 2–10 nm. A schematic model of the activation process has been presented, which includes electrochemical insertion of ions into the inside of the ASC. 相似文献
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