Nutritional response of mature rats to kidney bean (Phaseolus vulgaris) lectins

In a series of 10-day pair feeding experiments it was found that the nutritional value of diets containing beans was essentially the same for rats aged between 30 and 123 days. Thus net protein utilisation (NPU) values of 25–39 on diets containing Processor bean (35 g protein kg^?1) + egg albumin (65g protein kg^?1) were obtained. As food intakes were considerably reduced when rats were fed diets containing more than 35g protein kg^?1 of Processor bean, the measurement of protein utilisation became increasingly more difficult. The severe disruption of the brush borders of duodenal and jejunal enterocytes, originally observed when bean-containing diets were fed to young (30-day-old) rats was also found with rats up to the age of 120 days on similar diets. Similarly, the development of circulating anti-lectin antibodies in the animals showed no age dependence within the age limits investigated. It was also shown that oral immunisation did not protect the rats from the effects of toxicity and that the immune response was a result of continuous absorption of lectin throughout the feeding period. Thus the extent and the mechanism of toxicity of Phaseolus vulgaris bean lectins were found not to be dependent on the age or maturity of the animal.     

Potential applications of poly(vinyl alcohol)‐congo red aqueous solutions and hydrogels as liquids for hydraulic fracturing

The authors report on the viscoelastic characterization of guar hydrogels obtained through complexation reactions with borax ions. These gels are compared with hydrogels obtained from poly(vinyl alcohol) of different degree of hydrolysis through complexation reactions with congo red. The effect of the degree of hydrolysis and both, the concentration of PVA and the concentration of congo red, on the viscoelastic properties of the hydrogels is analyzed. The potential use of the PVA‐based hydrogels as hydraulic fracturing liquids is discussed in relation to the commonly used fracturing liquid based on the guar–borax system. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Cpk index estimation using data transformation

Process capability indices (PCIs) are used in industry to assess percentages of nonconforming parts. An underlying assumption is that the output process measurements are distributed as normal random variables. When normal distributions are assumed, but different distributions are present - such as skew, heavy-tailed, and short-tailed distributions - the percentages of nonconforming parts are significantly different than the computed PCIs indicate. Data arising from nonnormal distributions can sometimes be transformed to conform to the normality assumption and the PCI's computed for the transformed data. In this paper, the effect of the transformation on the estimate of nonconforming parts is examined for three examples of nonnormal distributions - gamma, lognormal, and Weibull. The results of this experimental analysis suggest that data transformation can be useful for estimating an interval for C_pk values and the number of nonconforming parts.     

Pre-tensioning of fabrics in cement-based composites

This paper studies the effects induced by applying a pre-tension load during the production of fabric-cement composites. The flexural behavior as well as the bonding between the different fabrics and the cement matrix was examined, as a means for characterizing the processing parameters. Microstructure characteristics of the fabric-matrix interface as well as the viscous elasticity properties of the fabrics were also explored and correlated with the mechanical properties of the composite. It was found that pre-tensioning of fabrics and the time at which the tension is removed can significantly influence the performance of the composite depending on yarn properties, mainly the viscous-elastic properties, and fabric geometry.     

Assay of aflatoxin in peanuts and peanut products using acetone-hexane-water for extraction

A quantitative method is described for the assay of aflatoxin in peanut products. The procedure involves extraction of aflatoxin from the sample with a homogeneous acetone-hexane-water solvent mixture followed by purification of the extract by phasic extraction of the aflatoxin with aqueous sodium chloride and then with chloroform. The purified chloroform extract is analyzed by thin-layer chromatography by comparison of the intensity of fluorescence of any aflatoxin with the intensity of a known standard. The aflatoxin analyses of peanuts were found to be very variable due to sampling, and this variability has been greatly reduced by finely grinding and thoroughly mixing 2 kg of the sample before removal of an aliquot for assay. The method is sensitive to approximately 2 parts per billion. Honorable mention, Bond Award competition. Presented at AOCS Meeting, Chicago, Illinois, October, 1964. So. Reg. Res. Lab., New Orleans, Louisiana, one of the laboratories of the So. Utiliz. Res. and Dev. Div., ARS, USDA.     

Chronic-Antibiotics Induced Gut Microbiota Dysbiosis Rescues Memory Impairment and Reduces β-Amyloid Aggregation in a Preclinical Alzheimer’s Disease Model

Alzheimer’s disease (AD) is a multifactorial pathology characterized by β-amyloid (Aβ) deposits, Tau hyperphosphorylation, neuroinflammatory response, and cognitive deficit. Changes in the bacterial gut microbiota (BGM) have been reported as a possible etiological factor of AD. We assessed in offspring (F1) 3xTg, the effect of BGM dysbiosisdysbiosis in mothers (F0) at gestation and F1 from lactation up to the age of 5 months on Aβ and Tau levels in the hippocampus, as well as on spatial memory at the early symptomatic stage of AD. We found that BGM dysbiosisdysbiosis with antibiotics (Abx) treatment in F0 was vertically transferred to their F1 3xTg mice, as observed on postnatal day (PD) 30 and 150. On PD150, we observed a delay in spatial memory impairment and Aβ deposits, but not in Tau and pTau protein in the hippocampus at the early symptomatic stage of AD. These effects are correlated with relative abundance of bacteria and alpha diversity, and are specific to bacterial consortia. Our results suggest that this specific BGM could reduce neuroinflammatory responses related to cerebral amyloidosis and cognitive deficit and activate metabolic pathways associated with the biosynthesis of triggering or protective molecules for AD.     

Content Management Systems: Enriched learning opportunities for all?

This article examines the popular claim of Content Management Systems (CMSs) that providing a rich toolset and leaving the use under learner control is beneficial to learning. By means of a literature review, the current contribution examines whether all students are capable of using CMS tools so that their learning is enhanced. In contrast to what is assumed, the study conceptualizes tool use as a complex self-regulation strategy that cannot be taken for granted. Specifically, the article reviews empirical studies in relation to three topics: (a) personal agency in tool use, (b) performance effects of tool use and (c) influencing tool use variables. Findings reveal that not every student profited from the CMS learning opportunities; in multiple studies students differed in their tool use, and these differences had significant performance effects. Hence, these findings suggest that the pedagogical claim CMSs make is problematic. Besides this accumulated corpus of knowledge, the review revealed serious limitations in the retrieved studies which could hamper our findings. As a consequence, the review establishes a need for further research into students’ CMS tool use from an instructional design perspective. In addition to the theoretical framework, several directions for future research are given.