全文获取类型
收费全文 | 1806篇 |
免费 | 31篇 |
专业分类
电工技术 | 17篇 |
综合类 | 3篇 |
化学工业 | 156篇 |
金属工艺 | 15篇 |
机械仪表 | 57篇 |
建筑科学 | 43篇 |
能源动力 | 46篇 |
轻工业 | 65篇 |
水利工程 | 10篇 |
石油天然气 | 1篇 |
无线电 | 152篇 |
一般工业技术 | 219篇 |
冶金工业 | 864篇 |
原子能技术 | 4篇 |
自动化技术 | 185篇 |
出版年
2023年 | 4篇 |
2022年 | 11篇 |
2021年 | 19篇 |
2020年 | 24篇 |
2019年 | 19篇 |
2018年 | 18篇 |
2017年 | 30篇 |
2016年 | 29篇 |
2015年 | 17篇 |
2014年 | 20篇 |
2013年 | 70篇 |
2012年 | 41篇 |
2011年 | 67篇 |
2010年 | 45篇 |
2009年 | 36篇 |
2008年 | 49篇 |
2007年 | 47篇 |
2006年 | 48篇 |
2005年 | 47篇 |
2004年 | 39篇 |
2003年 | 35篇 |
2002年 | 30篇 |
2001年 | 18篇 |
2000年 | 17篇 |
1999年 | 55篇 |
1998年 | 246篇 |
1997年 | 153篇 |
1996年 | 95篇 |
1995年 | 65篇 |
1994年 | 67篇 |
1993年 | 43篇 |
1992年 | 13篇 |
1991年 | 24篇 |
1990年 | 12篇 |
1989年 | 28篇 |
1988年 | 23篇 |
1987年 | 24篇 |
1986年 | 16篇 |
1985年 | 19篇 |
1984年 | 4篇 |
1983年 | 11篇 |
1982年 | 3篇 |
1981年 | 10篇 |
1980年 | 20篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 32篇 |
1976年 | 72篇 |
1975年 | 3篇 |
1974年 | 4篇 |
排序方式: 共有1837条查询结果,搜索用时 15 毫秒
1.
Husain Syed Sameed Ong Eng-Jon Bober Miroslaw 《International Journal of Computer Vision》2021,129(5):1432-1450
International Journal of Computer Vision - We propose a novel CNN architecture called ACTNET for robust instance image retrieval from large-scale datasets. Our key innovation is a learnable... 相似文献
2.
The metabolism of the poly(A) tail is a process important for the translational regulation of maternal mRNAs in Xenopus laevis oocytes and early embryos. Two poly(A) nuclease (PAN) activities have been described in Xenopus embryo or activated egg extracts (Legagneux et al (1995) RNA 1, 1001-1008). These activities (default PAN and EgPAN) are distinguishable by their deadenylation kinetics and their substrate specificities. In this report, we show that these activities display different sensitivities to biochemical treatments. Urea and, to a lesser extent, spermidine, inhibit EgPAN at concentrations which have no effect on default PAN. Heparin activates default PAN but inhibits EgPAN. When extracts are fractionated by ultracentrifugation, the default activity is recovered in one unique fraction, whereas two fractions must be combined to reconstitute the EgPAN activity. Moreover, these two deadenylation activities are separable by size exclusion chromatography under native conditions. We conclude that these two deadenylation activities are mediated by two protein complexes. 相似文献
3.
Production by N-nitroso compounds of O6-alkylguanine (O6-alkylG) in DNA directs the misincorporation of thymine during DNA replication, leading to G:C to A:T transition mutations, despite the fact that DNA containing O6-alkylG:T base pairs is less stable than that containing O6-alkylG:C pairs. We have examined the kinetics of incorporation by Klenow fragment (KF) of Escherichia coli DNA polymerase I of thymine (T) and of cytosine (C) opposite O6-MeG in the template DNA strand. Both T and C were incorporated opposite O6-MeG much slower than nucleotides forming regular A:T or G:C base pairs. Using various concentrations of dTTP, dCTP, or their phosphorothioate (Sp)-dNTP alpha S analogues, or a mixture of dTTP and dCTP, the progress of incorporation of a single nucleotide in a single catalytic cycle of a preformed KF-DNA complex was measured (pre-steady-state kinetics). The results were consistent with the kinetic scheme (Kuchta, R. D., Benkovic, P., & Benkovic, S. J. (1988) Biochemistry 27, 6716-6725): (1) binding of dNTP to polymerase-DNA; (2) conformational change in polymerase; (3) formation of phosphodiester between the dNTP and the 3'-OH of the primer; (4) conformational change of polymerase; (5) release of pyrophosphate. The results were analyzed mathematically to identify the steps at which the rate constants differ significantly between the incorporation of T and C. The only significant difference was the 5-fold difference in the rates of formation of the phosphodiester bond (for dTTP, kforward = 3.9 s-1 and kback = 1.9 s-1; for dCTP, kforward = 0.7 s-1 and kback = 0.9 s-1). These pre-steady-state progress curves were biphasic with a rapid initial burst followed by an apparently steady-state rise. Deconvolution of these curves gave direct evidence for the importance of the conformational change after polymerization by showing that the curves represented the sum of the rapid accumulation of the product of step 3 followed by the slow conversion of that to the product of step 5 (because of the rapidity of the release of pyrophosphate there was no significant accumulation of the product of step 4). The equilibrium constants for each step suggest that the greatest change in the Gibbs free energy occurs at the conformational change after polymerization and that while the formation of the phosphodiester bond to T is slightly exothermic, that to C is slightly endothermic.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
4.
Liang Y.C. Wenjiang Zeng Pick Hong Ong Zhaoxia Gao Jun Cai Balasubramanian N. 《Electron Device Letters, IEEE》2002,23(12):700-703
In this letter, a concise process technology is proposed for the first time to enable the fabrication of good quality three-dimensional (3-D) suspended radio frequency (RF) micro-inductors on bulk silicon, without utilizing the lithography process on sidewall and trench-bottom patterning. Samples were fabricated to demonstrate the applicability of the proposed process technology. 相似文献
5.
维拉诺瓦巴奎因城市公园坐落在里斯本市区,倚靠葡萄牙最长的河流——塔古斯河的右岸。在塔古斯河谷的大型综合项目中,包含了对这片10hm^2土地的改造,以重新评估它的城市和景观价值。 相似文献
6.
W. L. Siew F. C. H. Oh A. S. H. Ong 《Journal of the American Oil Chemists' Society》1985,62(6):1036-1039
Some of the factors affecting cloud point determination of palm oleins are described. These are the type of container used, method of stirring, rate of stirring and bath temperature. The repeatability and reproducibility standard deviations of the method are determined from collaborative trials. Recommendations for the test are made to reduce the large variations among laboratories. 相似文献
7.
The authors examine effects on the torque that simple variations of the standard rotor pole face profile could have, basically from an elementary viewpoint of how the modified profiles affect the distribution and magnitude of the radial and tangential components of the flux density. It is observed that the average torque is mostly affected by changes that alter the dominant radial component, such as changes in the effective air gap length. The other observation that an increase in the average torque can also be obtained by a favorable shift in the torque versus angle characteristics by relatively simple changes of the rotor pole profile is not so well known. Such a shift that reduces the slope of the torque angle characteristic, skewing the curve towards the unaligned position, has two advantages: one is that the phase inductance is at its maximum positive slop and, hence, the maximum torque, when the phase is energized; second, the flatter inductance profile near the aligned position when the phase current is to be commutated would allow a faster drop off of the commutated current and, thus, a smaller negative torque 相似文献
8.
9.
The three-dimensional solution structure of des-[Phe(B25)] human insulin has been determined by nuclear magnetic resonance spectroscopy and restrained molecular dynamics calculations. Thirty-five structures were calculated by distance geometry from 581 nuclear Overhauser enhancement-derived distance constraints, ten phi torsional angle restraints, the restraints from 16 helical hydrogen bonds, and three disulfide bridges. The distance geometry structures were optimized using simulated annealing and restrained energy minimization. The average root-mean-square (r.m.s.) deviation for the best 20 refined structures is 1.07 angstroms for the backbone and 1.92 angstroms for all atoms if the less well-defined N and C-terminal residues are excluded. The helical regions are more well defined, with r.m.s. deviations of 0.64 angstroms for the backbone and 1.51 angstroms for all atoms. It is found that the des-[Phe(B25)] insulin is a monomer under the applied conditions (4.6 to 4.7 mM, pH 3.0, 310 K), that the overall secondary and tertiary structures of the monomers in the 2Zn crystal hexamer of native insulin are preserved, and that the conformation-averaged NMR solution structure is close to the structure of molecule 1 in the hexamer. The structure reveals that the lost ability of des-[Phe(B25)] insulin to self-associate is caused by a conformational change of the C-terminal region of the B-chain, which results in an intra-molecular hydrophobic interaction between Pro(B28) and the hydrophobic region Leu(B11)-Leu(B15) of the B-chain alpha-helix. This interaction interferes with the inter-molecular hydrophobic interactions responsible for the dimerization of native insulin, depriving the mutant of the ability to dimerize. Further, the structure displays a series of features that may explain the high potency of the mutant on the basis of the current model for the insulin-receptor interaction. These features are: a change in conformation of the C-terminal region of the B-chain, the absence of strong hydrogen bonds between this region and the rest of the molecule, and a relatively easy accessibility to the Val(A3) residue. 相似文献
10.