Information Sharing Among Disaster Responders - An Interactive Spreadsheet-Based Collaboration Approach

Recent natural disasters have led crisis management organizations to revise their protocols so as to rely on the contribution of a wider range of actors, including simple citizens as well as expert operators, to support decision making activities. Reliable and timely information sharing among members of distributed teams of disaster responders has become paramount for the success of the overall crisis management process. In this paper we propose a crisis management system based on spreadsheet-mediated collaboration among on-site responders and decision makers. To share data a common spreadsheet artifact has been developed by using a participatory design approach which is accessed through mobile user interfaces. The evaluation results showed that the use of the spreadsheet artifact has resulted in more effective decision making relating to set of earthquake management scenarios in high-risk areas located in Italy.     

Multidrug Idebenone/Naproxen Co-loaded Aspasomes for Significant in vivo Anti-inflammatory Activity

The use of proper nanocarriers for dermal and transdermal delivery of anti-inflammatory drugs recently gained several attentions in the scientific community because they pass intact and accumulate payloads in the deepest layers of skin tissue. Ascorbyl palmitate-based vesicles (aspasomes) can be considered a promising nanocarrier for dermal and transdermal delivery due to their skin whitening properties and suitable delivery of payloads through the skin. The aim of this study was the synthesis of multidrug Idebenone/naproxen co-loaded aspasomes for the development of an effective anti-inflammatory nanomedicine. Aspasomes had suitable physicochemical properties and were safe in vivo if topically applied on human healthy volunteers. Idebenone/naproxen co-loaded aspasomes demonstrated an increased therapeutic efficacy of payloads compared to the commercially available Naprosyn® gel, with a rapid decrease of chemical-induced erythema on human volunteers. These promising results strongly suggested a potential application of Idebenone/naproxen multidrug aspasomes for the development of an effective skin anti-inflammatory therapy.     

S‐N curves in the very‐high‐cycle fatigue regime: statistical modeling based on the hydrogen embrittlement consideration

The continuous increment of durability and reliability requirements for many machinery components is significantly enhancing the research activity in the Very‐High‐Cycle Fatigue (VHCF) characterization of metallic materials, in particular of high‐strength steels for critical structural applications. According to the model, the VHCF strength of high‐strength steels can be estimated from the projected area of the ‘Optically Dark Area’ (ODA), which plays a key role in the VHCF response of high‐strength steels: more than 95% of the total VHCF life is consumed in the ODA formation, with crack growing even though the Stress Intensity Factor (SIF) is below the threshold for crack growth. Following the hydrogen embrittlement theory proposed by Murakami, hydrogen is supposed to assist crack growth within the ODA. The present paper proposes a general SIF formulation for the analytical model of the hydrogen assisted crack growth within the ODA. Starting from the general SIF formulation, a general expression for the material fatigue limit is obtained in the paper. The statistical method for the estimation of the parameters involved in the proposed model is finally illustrated in the paper and numerically applied to an experimental dataset.     

Antifungal activity of Rubus ulmifolius Schott standardized in vitro culture

Vegetables of Rubus species are known to have antimicrobial properties mainly due to its high content in phenolic compounds. Among the 37 potentially pathogenic fungi screened with crude methanolic extract from micropropagated shoots of Rubus ulmifolius Schott, we show that the 70% of the species resulted variably sensitive to methanolic extract. Of the phenolic fractions obtained from the crude methanolic extract by hydrophobic interaction chromatography, those rich in tannins resulted active against the fungal strains tested, whereas those containing chlorogenic, caftaric acid and caffeoyl derivatives resulted partially effective. Our results suggest a potential utilization of the in vitro plant cultures of R. ulmifolius to provide standardized extracts from plant material grown in uniform conditions and available year round, which could be useful as natural antifungal agents.     

Bronchodilator response to salbutamol after spontaneous recovery from nonspecific bronchial provocation tests in asthma

Assessment of airway responsiveness by bronchoprovocation and bronchodilatation tests is important in the diagnostic work-up protocol of bronchial asthma and it would be convenient to undertake both tests on the same occasion. However, it is not known whether this can be done accurately. Therefore, this study evaluated the effect of a prior bronchial provocation test on the bronchodilator response to salbutamol after spontaneous recovery of the forced expiratory volume in one second (FEV1) in a group of asthmatic subjects. On two separate occasions at the same time of day, concentration-response studies with inhaled histamine or methacholine, or a sham challenge with normal saline were carried out in a blinded, randomized manner. Changes in airway calibre were followed as FEV1 and agonist responsiveness expressed as the provocative concentration causing a 20% fall in FEV1 (PC20). After either spontaneous recovery or a fixed-duration wait of 45 min (when appropriate), the subjects received 2x100 microg of salbutamol from a metered dose inhaler with a spacer. The bronchodilator response to salbutamol was expressed as a percentage of initial FEV1 (deltaFEV1% init). Bronchial challenge with both agonists failed to alter significantly the airway response to salbutamol, with the deltaFEV1% init mean value (range) being 16.9% (9.0-31.9) and 17.5% (11.6-31.2) on the sham and histamine/methacholine challenge day respectively. It was shown that the degree of bronchodilatation achieved after salbutamol 200 microg is not affected by prior bronchoprovocation testing when enough time is allowed for the airways to recover spontaneously to baseline forced expiratory volume in one second. Thus evaluation of airway responsiveness by both bronchial provocation tests and bronchodilator testing can be assessed reliably within a few hours in asthmatic patients.     

Impact response of adhesive reversible joints made of thermoplastic nanomodified adhesive

The impact response of a reversible adhesive joint is experimentally assessed in this work. Joint reversibility is improved with a system that uses nanomodification of the thermoplastic adhesive used for bonding plastic components. This system, coupled with electromagnetic induction, is able to guarantee the separation of joints without any damage to the substrates. Drop dart tests at different impact velocities are carried out on neat and nanomodified bonded joints in order to compare the impact behavior before and after the introduction of nanoparticles. Experimental results show that the impact response, assessed in terms of peak load, absorbed energy, and flexural stiffness, can be affected by the introduction of nanoparticles. This work shows that adhesive nanomodified joints represent an effective and applicable solution for the reversible assembling of semi-structural components subjected to low-velocity impact loads.     

Improved in vitro anti-tumoral activity, intracellular uptake and apoptotic induction of gemcitabine-loaded pegylated unilamellar liposomes

Anaplastic thyroid carcinoma is one of the most aggressive and lethal solid carcinomas affecting humans. A major limit of the chemotherapeutic agents is represented by their low therapeutic index. In this work, we investigated the possibility of improving the anti-tumoral activity of gemcitabine by using pegylated unilamellar liposomes. Liposomes were made up of 1,2-dipalmitoyl-sn-glycero-3-phospocholine monohydrate/cholesterol/N-(carbonyl-methoxypolyethylene glycol-2000)-1, 2-distearoyl-sn-glycero-3-phosphoethanolamine (6:3:1 molar ratio) and they were prepared with a pH gradient to improve the gemcitabine loading capacity. The anti-tumoral efficacy of the liposomal formulation was tested in vitro on human anaplastic thyroid carcinoma cells (ARO) in culture, comparing the effects with those of the free drug. Gemcitabine-loaded unilamellar liposomes had a mean size approximately 200 nm with a zeta potential approximately -2 mV. The liposomal carrier noticeably improved the anti-tumoral activity of gemcitabine against ARO cells in terms of both dose-dependent cytotoxic effect and of drug exposition effect. Namely, gemcitabine-loaded liposomes showed a cytotoxic effect (58.2% increase of cell mortality at 1 microM with respect to free drug) after 12 h incubation, while the free drug showed a significant activity only after 72 h incubation. Moreover, a significant effect on the cell mortality appeared at 0.1 microM and 100% mortality was detected at a concentration of 1 microM of gemcitabine-loaded liposomes, while the free drug elicited the same effect at a concentration of 100 microM. The improved anti-tumoral activity of gemcitabine determined by the liposomal carrier was due to a greater intracellular uptake. The intracellular gemcitabine levels as a function of time showed a sinusoidal profile with peaks after 2 h, 6 h and 11 h, related to the cellular cycle of ARO. PARP cleavage and DNA fragmentation analysis provided clear evidence of the apoptosis induction in ARO cells by treatment with liposomally entrapped gemcitabine after 72 h incubation. Thus, gemcitabine-loaded liposomes may have a potential therapeutic relevance for the treatment of anaplastic thyroid carcinoma.     

On the rate of growth and extent of the steady damage accumulation phase in repeated impact tests

In the paper, data of repeated impact tests performed on seven laminates of different lay-up and thickness are used to illustrate how the damage index (DI), a damage variable recently introduced by the authors to monitor the range of the penetration process in thick laminates, can be applied in case of repeated impact tests to get information on the rate of initial steady damage accumulation as well as on the onset of severe damage modes.Curves of the rate of growth of the DI in the steady phase (ΔDI/ΔN) vs. the normalised impact energy (ratio of the impact energy E_i and the laminate penetration threshold P_n) show no significant damage accumulation besides initial specimen indentation for impact energies below 0.2P_n. For intermediate levels of impact energy, repeated impact tests are characterised by an initial region of steady damage accumulation followed by an abrupt change in the rate of damage growth a few impacts before laminate perforation. For higher impact energies (E_i/P_n > 0.4–0.45), no phase of steady damage accumulation is present, suggesting that severe damage mechanisms take place from the very first impacts. Values of the DI at the end of the steady phase (DI_unsteady) are shown to be rather peculiar to each laminate regardless of the impact energy used in the tests and therefore may be used to get a first indication of the laminate performance to repeated impacts. The extent of the steady phase may also be used to compute the total energy absorbed by the laminate in the steady damage accumulation phase (E_aTOT__steady).     

Comparison of bioactive phytochemical content and release of isothiocyanates in selected brassica sprouts

The consumption of brassica sprouts as raw vegetables provides a fair amount of glucosinolates (GLs) and active plant myrosinase, which enables the breakdown of GLs into health-promoting isothiocyanates (ITCs). This study reports the determination of the main constituents related to human health found in edible sprouts of two Brassica oleracea varieties, broccoli and Tuscan black kale, and two Raphanus sativus varieties, Daikon and Sango. Radish sprouts exhibited the highest ability to produce ITCs, with Daikon showing the greatest level of conversion of GLs into bioactive ITCs (96.5%), followed by Sango (90.0%). Tuscan black kale gave a value of 68.5%, whereas broccoli displayed the lowest with 18.7%. ITCs were not the exclusive GL breakdown products in the two B. oleracea varieties, since nitriles were also produced, thus accounting for the lower conversion observed. Measuring the release of plant ITCs is a valuable tool in predicting the potential level of exposure to these bioactive compounds after the consumption of raw brassica sprouts.