Favorable influence of ssDNA-functionalized SWCNT on the navigation pattern of C. elegans

Microsystem Technologies - Single-Walled Carbon Nanotubes (SWCNTs) are widely used as potential carriers in drug delivery systems. The objective of this work was to observe the effects of pristine,...     

In Vitro Characterization of Human CD24hiCD38hi Regulatory B Cells Shows CD9 Is Not a Stable Breg Cell Marker

Regulatory B (Breg) cells are endowed with immune suppressive functions. Various human and murine Breg subtypes have been reported. While interleukin (IL)-10 intracellular staining remains the most reliable way to identify Breg cells, this technique hinders further essential functional studies. Recent findings suggest that CD9 is an effective surface marker of murine IL-10 competent Breg cells. However, the stability of CD9 and its relevance as a unique marker for human Breg cells, which have been widely characterized as CD24^hiCD38^hi, have not been investigated. Here, we demonstrate that CD9 expression is sensitive to in vitro B cell stimulations. CD9 expression could either be re-expressed or downregulated in purified CD9-negative B cells and CD9-positive B cells, respectively. We found no significant differences in the Breg differentiation capacity of the CD9-negative and CD9-positive B cells. Furthermore, CD9-positive B cells co-express CD40 and CD86, suggesting their nature as B cell activation or co-stimulatory molecules, rather than regulatory ones. Therefore, we report the relatively unstable CD9 as a distinct surface molecule, indicating the need for further research for a more reliable marker to purify human Breg cells.     

Low intensity visual data improvement using DWT and LWT based regularizer

Image Completion plays a vital role in compressed sensing, machine learning, and computer vision applications. The Rank Minimization algorithms are used to perform the image completion. The major problem with rank minimization algorithms is the loss of information in the recovered image at high corruption ratios. To overcome this problem Lifting wavelet transform based Rank Minimization (LwRM), and Discrete wavelet transform based Rank Minimization (DwRM) methods are proposed, which can recover the image, if the corrupted observations are more than 80%. The evaluation of the proposed methods are accomplished by Full Reference Image Quality Assessment (FRIQA) and No Reference Image Quality Assessment (NR-IQA) metrics. The simulation results of proposed methods are superior to state-of-the-art methods.

     

Biocompatible antimicrobial cotton fibres for healthcare industries: a biogenic approach for synthesis of bio‐organic‐coated silver nanoparticles

Cotton fibres coated with biogenically fabricated silver nanoparticles (SNPs) are most sought material because of their enhanced activity and biocompatibility. After successful synthesis of SNPs on cotton fibres using leaf extract of Vitex negundo Linn, the fibres were studied using diffuse reflectance spectroscopy, scanning electron microscopy, nanoparticle tracking analysis, energy dispersive X‐ray, and inductively coupled plasma atomic emission spectrometry. The characterisation revealed uniformly distributed spherical agglomerates of SNPs having individual particle size around 50 nm with the deposition load of 423 μg of silver per gram of cotton. Antimicrobial assay of cotton–SNPs fibres showed effective performance against pathogenic bacteria and fungi. The method is biogenic, environmentally benign, rapid, and cost‐effective, producing highly biocompatible antimicrobial coating required for the healthcare industry.Inspec keywords: cotton, health care, nanoparticles, coatings, silver, fibres, nanofabrication, scanning electron microscopy, X‐ray chemical analysis, atomic emission spectroscopy, plasma applications, microorganisms, biotechnologyOther keywords: biocompatible antimicrobial cotton fibre coating, healthcare industry, bioorganic‐coated silver nanoparticle synthesis, biogenically fabricated silver nanoparticle, SNP, leaf extraction, Vitex negundo Linn, diffuse reflectance spectroscopy, scanning electron microscopy, nanoparticle tracking analysis, energy dispersive X‐ray spectrometry, inductively coupled plasma atomic emission spectrometry, uniformly distributed spherical agglomerate, antimicrobial assay, pathogenic bacteria, fungi, Ag     

Shifting of research trends in islanding detection method - a comprehensive survey

The augmentation in electricity demand, power system privatization as well as efficacy of renewable resources has paved the way for power system companies and researchers to exploit the field of grid connected distributed generation (DG) and its issues, islanding being a dominant one. Several research works have been conducted to mitigate the issues of islanding detection (ID). In context of this, the paper gives a comprehensive review of islanding issues, standard test systems, criteria and shifting of research trends in islanding detection methods (IDMs). The significant contributions pertain to categorization of IDMs, evaluation of non-detection zone (NDZ) for each test system, disquisition on evolution and advancement of IDMs and its comparisons based on criteria such as NDZ, run on time, nuisance tripping percentage, applicability in multi DG system and implementation cost to draw out the strength and shortcomings of individual methods that will come to aid to the companies or researchers for establishing the applicability and appropriateness of such method for their concerned domain.     

DOT1L Methyltransferase Regulates Calcium Influx in Erythroid Progenitor Cells in Response to Erythropoietin

Erythropoietin (EPO) signaling plays a vital role in erythropoiesis by regulating proliferation and lineage-specific differentiation of murine hematopoietic progenitor cells (HPCs). An important downstream response of EPO signaling is calcium (Ca²⁺) influx, which is regulated by transient receptor potential channel (TRPC) proteins, particularly TRPC2 and TRPC6. While EPO induces Ca²⁺ influx through TRPC2, TRPC6 inhibits the function of TRPC2. Thus, interactions between TRPC2 and TRPC6 regulate the rate of Ca²⁺ influx in EPO-induced erythropoiesis. In this study, we observed that the expression of TRPC6 in KIT-positive erythroid progenitor cells was regulated by DOT1L. DOT1L is a methyltransferase that plays an important role in many biological processes during embryonic development including early erythropoiesis. We previously reported that Dot1l knockout (Dot1l^KO) HPCs in the yolk sac failed to develop properly, which resulted in lethal anemia. In this study, we detected a marked downregulation of Trpc6 gene expression in Dot1l^KO progenitor cells in the yolk sac compared to the wild type (WT). The promoter and the proximal regions of the Trpc6 gene locus exhibited an enrichment of H3K79 methylation, which is mediated solely by DOT1L. However, the expression of Trpc2, the positive regulator of Ca²⁺ influx, remained unchanged, resulting in an increased TRPC2/TRPC6 ratio. As the loss of DOT1L decreased TRPC6, which inhibited Ca²⁺ influx by TRPC2, Dot1l^KO HPCs in the yolk sac exhibited accelerated and sustained elevated levels of Ca²⁺ influx. Such heightened Ca²⁺ levels might have detrimental effects on the growth and proliferation of HPCs in response to EPO.     

Separation of acid–water mixtures by pervaporation using nanoparticle filled mixed matrix copolymer membranes

BACKGROUND: Low energy and less expensive membrane based separation of acetic acid‐water mixtures would be a better alternative to conventional separation processes. However, suitable acid resistant membranes are still lacking. Thus, the objective of the present study was to develop mixed matrix membrane (MMM) which would allow high flux and water selectivity over a wide range of feed concentrations of acid in water. RESULTS: Three MMMs, namely PANBA0.5, PANBA1.5 and PANBA3 were made by emulsion copolymerization of acrylonitrile (AN) and butyl acrylate (BA) with 5.5:1 comonomer ratio and in situ incorporation of 0.5, 1.5 and 3 wt%, sodium montmorilonite (Na‐MMT) nanofillers, respectively. For a feed concentration of 99.5 wt% of acid in water the membranes show good permeation flux (2.61, 3.19, 3.97 kg m^?2 h^?1 µm^?1, for PANBA0.5, PANBA1.5 and PANBA3 membrane, respectively) and very high separation factors for water (1473, 1370, 1292 for PANBA0.5, PANBA1.5 and PANBA3 membrane, respectively) at 30 °C. Similarly for a dilute acid–water solution, i.e. for 71.6 wt% acid the membrane showed a very high thickness normalize flux (8.67, 9.44, 11.56 kg m^?2 h^?1 µm^?1, for PANBA0.5, PANBA1.5 and PANBA3 membrane, respectively) and good water selectivity (101.7, 95.3, 79 for PANBA0.5, PANBA1.5 and PANBA3 membrane, respectively) at the same feed temperature. The permeation ratio, permeability, diffusion coefficient and activation energy for permeation of the membranes were also estimated. CONCLUSION: Unlike most of the reported membranes, the present MMMs allowed high flux and selectivity over a wide range of feed concentrations. These membranes may also be effective for separating other similar organic‐water mixtures. Copyright © 2012 Society of Chemical Industry     

Plug-load densities for energy analysis: K-12 schools

Benchmarking plug-load densities is essential to bypass arbitrary and/or incorrect inputs used in building energy analysis. As more building simulationists play a decision-making role for the design team, they tend to lean on building energy standards and guidelines for preliminary inputs such as plug-load densities. It is necessary for building energy standards and rating systems to implement plug-load density benchmarks to reward design teams in their efforts to reduce plug-load energy use. Using case study buildings, this paper establishes benchmark plug-load densities for K-12 schools under two new categories – classrooms with computers and classrooms without computers. Eighteen K-12 schools including 9 elementary, 2 middle, and 7 high schools are assessed for actual plug-load densities. For the same case study buildings, four existing approaches – NREL, COMNET, ASHRAE 90.1-1989, and Title-24 are evaluated for plug-load densities. Results show under- and over-estimation of plug-load densities over actual densities. The development of benchmark for K-12 schools will pave way for instituting targets for trimming plug-load densities in new and retrofit building projects.