全文获取类型
收费全文 | 115篇 |
免费 | 13篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 3篇 |
化学工业 | 32篇 |
金属工艺 | 8篇 |
机械仪表 | 3篇 |
建筑科学 | 2篇 |
能源动力 | 7篇 |
轻工业 | 24篇 |
水利工程 | 2篇 |
石油天然气 | 2篇 |
无线电 | 3篇 |
一般工业技术 | 18篇 |
冶金工业 | 11篇 |
自动化技术 | 15篇 |
出版年
2024年 | 1篇 |
2023年 | 6篇 |
2022年 | 8篇 |
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 7篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 13篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 9篇 |
2012年 | 7篇 |
2011年 | 12篇 |
2010年 | 11篇 |
2009年 | 13篇 |
2008年 | 3篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 1篇 |
2001年 | 1篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有130条查询结果,搜索用时 15 毫秒
1.
Sofia Cotton Dylan Ferreira Janine Soares Andreia Peixoto Marta Relvas-Santos Rita Azevedo Paulina Piairo Lorena Diguez Carlos Palmeira Luís Lima Andr M. N. Silva Lúcio Lara Santos Jos Alexandre Ferreira 《International journal of molecular sciences》2021,22(4)
Esophageal cancer (EC) is a life-threatening disease, demanding the discovery of new biomarkers and molecular targets for precision oncology. Aberrantly glycosylated proteins hold tremendous potential towards this objective. In the current study, a series of esophageal squamous cell carcinomas (ESCC) and EC-derived circulating tumor cells (CTCs) were screened by immunoassays for the sialyl-Tn (STn) antigen, a glycan rarely expressed in healthy tissues and widely observed in aggressive gastrointestinal cancers. An ESCC cell model was glycoengineered to express STn and characterized in relation to cell proliferation and invasion in vitro. STn was found to be widely present in ESCC (70% of tumors) and in CTCs in 20% of patients, being associated with general recurrence and reduced survival. Furthermore, STn expression in ESCC cells increased invasion in vitro, while reducing cancer cells proliferation. In parallel, an ESCC mass spectrometry-based proteomics dataset, obtained from the PRIDE database, was comprehensively interrogated for abnormally glycosylated proteins. Data integration with the Target Score, an algorithm developed in-house, pinpointed the glucose transporter type 1 (GLUT1) as a biomarker of poor prognosis. GLUT1-STn glycoproteoforms were latter identified in tumor tissues in patients facing worst prognosis. Furthermore, healthy human tissues analysis suggested that STn glycosylation provided cancer specificity to GLUT1. In conclusion, STn is a biomarker of worst prognosis in EC and GLUT1-STn glycoforms may be used to increase its specificity on the stratification and targeting of aggressive ESCC forms. 相似文献
2.
3.
B.B. Aragão S.C. Trajano J.G. Silva B.P. Silva R.P. Oliveira J. W. Pinheiro Junior R.M. Peixoto R.A. Mota 《Journal of dairy science》2019,102(8):6923-6927
Reports of β-lactam-resistant Staphylococcus aureus in artisanal goat cheese are increasing, and this phenomenon is relevant to public health. The objective of the present study was to determine the prevalence of S. aureus strains carrying the blaZ and mecA resistance genes, as well as the genes encoding the staphylococcal enterotoxins SEA, SEB, SEC, SED, SEE, and TSST-1 in artisanal coalho cheese made from goat milk produced in northeastern Brazil. We used biochemical and molecular tests to characterize 54 S. aureus isolates found in artisanal coalho cheese collected from commercial establishments producing animal products in 11 municipalities of Pernambuco State, Brazil. A PCR analysis revealed that 42.6% (23/54) of the isolates were positive for the blaZ gene, and 7.4% (4/54) were resistant to methicillin by phenotypic testing. We did not detect mecA or any genes encoding enterotoxins. The presence of S. aureus carriers of the blaZ gene and the identification of methicillin-resistant S. aureus strains are of concern for the health of consumers of this type of cheese. 相似文献
4.
Fernanda P. Disconzi Wellington Balmant Jose Viriato Coelho Vargas Pedro H.R. Peixoto Dhyogo M. Taher Andre B. Mariano 《国际能源研究杂志》2019,43(8):3421-3435
The aim of the present work was to develop a transient mathematical model focused on microalgae biomass drying, considering two phases: solid (wet biomass) and gas (drying air). Mass and thermal energy balances were written for each phase producing a system of ordinary differential equations (ODE). The solution of the ODE set delivers the temperature and air humidity ratio and biomass profiles with respect to time. The numerical results were directly compared with temperature experimental measurements—for both phases—and with the biomass humidity content. Data from experiment 1 were used to carry out the mathematical model adjustment, whereas data from experiment 2 were used for the experimental validation of the model. The model was adjusted by proposing a new correlation for the mass transfer coefficient and by calibrating the heat transfer coefficient. The transient numerical results were in good quantitative and qualitative agreement with the experimental results, ie, within the experimental error bars. Then the experimentally validated mathematical model was utilized to optimize the following parameters: (i) the electric heater power ( ) and the dry air mass flow rate ( ) and (ii) the convection oven length to width ratio (L/W). The goal was to minimize system energy consumption (objective function). The optimization procedure was subject to the following physical constraints: (i) fixed convection oven total volume and (ii) fixed biomass and drying air contact surface area. For the oven original geometry, = 3.0 kW and = 9 g s?1 were numerically found for minimum energy consumption, so that 36.9% and 43.5% energy consumption decreases were obtained, respectively, in comparison with the measurements of experiment 1. Next, the numerical geometric optimization found (L/W)opt = 9, with and , which was capable to reach a 51.6% energy consumption reduction in comparison with the original system tested in experiment 1. The novelty of this work consists of the development and experimental validation of a physically based microalgae biomass drying mathematical model, ie, instead of using empirical correlations to predict the drying time and temperature profiles and then minimize system energy consumption. Therefore, the results show that it is reasonable to state that the model could be used to design, control, and optimize drying systems with configurations similar to the one analyzed in this study. 相似文献
5.
Nathalia Peixoto Hossein Ghaffari NikHamid Charkhkar 《Computer methods and programs in biomedicine》2013
People without disabilities seamlessly control devices with their hands. Interestingly, their hands can perform coarse and fine control. Implementing smooth control for computerized systems is not straightforward and most of the time it is not intuitive either. Here we offer a solution to that problem: smooth control through humming. Voice commands have become ubiquitous in modern technology. Speech-to-text applications abound. Smooth control, on the other hand, has not been tackled yet. Here we design and implement a humming control technique, and demonstrate a hardware implementation with a powered wheelchair. Once actuated, the speed with which the chair moves will depend on the subtle variation on the fundamental frequency of the user's humming, acquired through an accelerometer measuring vocal cord vibration. We also discuss two signal processing techniques that handle commonly encountered issues when trying to resolve frequencies in real time data. The hardware implementation shows performance of 80% and higher in speech recognition for signal-to-noise ratio (SNR) higher than 8 dB and 100% in smooth control and frequency detection for all tested SNRs. We also discuss potential applications of smooth humming control to other assistive technology. 相似文献
6.
Lia-Tânia Dinis Maria Manuela OliveiraJosé Almeida Rita CostaJosé Gomes-Laranjo Francisco Peixoto 《Food chemistry》2012,132(1):1-8
The antioxidant properties of different ecotypes of chestnut nut (cv. Judia) were studied. Total phenolics and flavonoids were also determinated. Total phenolics amount ranged from 9.6 mg/g of GAE (hottest ecotype, Murça) to 19.4 mg/g of GAE (coldest ecotype, Valpaços). Gallic and ellagic acid were the predominant compounds and Valpaços had the highest values while, Murça had the lowest ones. The antioxidant capacity of ethanolic extracts were evaluated through several biochemical essays: ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging activity, FRAP (ferric reducing/antioxidant power) and inhibition of oxidative haemolysis in erythrocytes. In order to evaluate the antioxidant efficiency of each ecotype, the EC50 values were calculated. Once again Valpaços revealed the best antioxidant properties, presenting much lower EC50 values. Climatic conditions influence seems to be a limiting factor for production of phenolic compounds and consequently for the antioxidant properties of chestnut nuts. 相似文献
7.
Cassio Eduardo Lima de Paiva Jose Lulz Antunes de Oliveira e Sousa Luiz Carlos de Almeida Paulo RobertoAguiar Luiz Femando de Melo Correia Juliana Silva Watanabe Rodrigo Moreira de Carvalho Creso deFranco Peixoto Denis Palomo Paschoalin 《土木工程与建筑:英文版》2013,(8):964-972
The EFVM (Vitoria Minas Railroad) is one of the main railways in Brazil. It transports freight trains of ore, 220 wagons each. These wagons have 2 boogies of 2 axles each and 32 metric tons on metre gauge. Elastic strains were measured on a special part of this railway due to these trains. The main load to evaluate stresses and strains was a G 16 Locomotive, a C-C kind from Vale, a Brazilian Company. The measurements were obtained by dynamic deflectometer installed on a main line of this railway, near Ipatinga, a city from Minas Gerais, one of Brazil states. This track was equipped to obtain stresses under an equal repeated static load A simulation of the stresses was made under critical strain by Ferrovia 1.0 software. It was also made an evaluation of unequal results from neighbor sleepers taking in comparison two equipped parts of this railway, one with compacted ballast and no compaction to the other. The results were strain limited, avoiding breakage or damage to the studied rails. This work analyses these measurements focusing on the improvement of track quality. 相似文献
8.
Paulo Santos Diogo Peixoto Inês Ferreira Ricardo Passos Pedro Pires Marco Simes Pedro Pouso-Ferreira Teresa Baptista Benjamín Costas 《International journal of molecular sciences》2022,23(3)
Photobacteriosis is a septicaemic bacterial disease affecting several marine species around the globe, resulting in significant economic losses. Although many studies have been performed related to the pathogen virulence and resistance factors, information regarding the host defence mechanisms activated once an infection takes place is still scarce. The present study was designed to understand innate immune responses of farmed juvenile gilthead seabream (Sparus aurata) after Photobacterium damselae subsp. piscicida (Phdp) infection. Therefore, two groups of seabream juveniles were intraperitoneally injected with 100 µL of PBS (placebo) or 100 µL of exponentially growing Phdp (1 × 106 CFU/mL; infected). The blood, plasma, liver, and head kidney of six fish from each treatment were sampled immediately before infection and 3, 6, 9, 24 and 48 h after infection for the broad screening of fish immune and oxidative stress responses. Infected animals presented marked anaemia, neutrophilia and monocytosis, conditions that are correlated with an increased expression of genes related to inflammation and phagocytic activity. Similar studies with different fish species and bacteria can be useful for the definition of health biomarkers that might help fish farmers to prevent the occurrence of such diseases. 相似文献
9.
Leandro C. Peixoto 《Journal of power sources》2009,192(2):724-729
The aim of this study was to evaluate the effect of solidification cooling rates on the as-cast microstructural morphologies of a Pb-1 wt%Sn alloy, and to correlate the resulting microstructure with the corresponding electrochemical corrosion resistance in a 0.5 M H2SO4 solution at 25 °C. Cylindrical low-carbon steel and insulating molds were employed permitting the two extremes of a significant range of solidification cooling rates to be experimentally examined. Electrochemical impedance spectroscopy (EIS) diagrams, potentiodynamic polarization curves and an equivalent circuit analysis were used to evaluate the electrochemical corrosion response of Pb-1 wt%Sn alloy samples. It was found that lower cooling rates are associated with coarse cellular arrays which result in better corrosion resistance than fine cells which are related to high cooling rates. The experimental results have shown that that the pre-programming of microstructure cell size of Pb-Sn alloys can be used as an alternative way to produce as-cast components of lead-acid batteries with higher corrosion resistance. 相似文献
10.
Luciane Madureira Almeida Juliana Ferreira Floriano Thuanne Pires Ribeiro Lais Nogueira Magno Lígia Souza Lima Silveira da Mota Nei Peixoto Fátima Mrué Paulo Melo-Reis Ruy de Souza Lino Junior Carlos Frederico de Oliveira Graeff Pablo José Gonçalves 《Journal of materials science. Materials in medicine》2014,25(9):2153-2162
The latex obtained from Hancornia speciosa is used in folk medicine for treatment of several diseases, such as acne, warts, diabetes, gastritis and inflammation. In this work, we describe the biocompatibility assessment and angiogenic properties of H. speciosa latex and its potential application in medicine. The physical–chemical characterization was carried out following different methodologies (CHN elemental analyses; thermogravimetric analyses and Fourier transform infrared spectroscopy). The biocompatibility was evaluated through cytotoxicity and genotoxicity tests in fibroblast mouse cells and the angiogenic properties were evaluated using the chick chorioallantoic membrane (CAM) assay model. The physical–chemical results showed that the structure of Hancornia speciosa latex biomembrane is very similar to that of Hevea brasiliensis (commercially available product). Moreover, the cytotoxicity and genotoxicity assays showed that H. speciosa latex is biocompatible with life systems and can be a good biomaterial for medical applications. The CAM test showed the efficient ability of H. speciosa latex in neovascularization of tissues. The histological analysis was in accordance with the results obtained in the CAM assay. Our data indicate that the latex obtained from H. speciosa and eluted in water showed significant angiogenic activity without any cytotoxic or genotoxic effects on life systems. The same did not occur with H. speciosa latex stabilized with ammonia. Addition of ammonia does not have significant effects on the structure of biomembranes, but showed a smaller cell survival and a significant genotoxicity effect. This study contributes to the understanding of the potentialities of H. speciosa latex as a source of new phytomedicines. 相似文献