Prebiotic synthesis of sequential peptides on the Hadean beach by a molecular engine working with nitrogen oxides as energy sources

Addressing the still open question of the prebiotic origin of sequential macromolecules (peptides, nucleic acids) on the primitive Earth, we describe a molecular engine (the primary pump), which works at ambient temperature and continuously generates, elongates and complexifies sequential peptides. This new scenario is based on a cyclic reaction sequence, whose keystep is the activation of amino acids into their N‐carboxyanhydrides (NCA) through nitrosation by NOx. This process could have taken place on tidal beaches; it requires a buffered ocean, emerged land and a nitrosating atmosphere. With the help of geochemical studies and computer simulations of atmosphere photochemistry, we show that the primitive Earth during the Hadean may have satisfied all these requirements. © 2001 Society of Chemical Industry.     

Phylogeny of trichomonads based on partial sequences of large subunit rRNA and on cladistic analysis of morphological data

Several domains of large subunit rRNA from nine trichomonad species have been sequenced. Molecular phylogenies obtained with parsimony and distance methods demonstrate the trichomonads are a monophyletic group which branches very early in the eukaryotic tree. The topology of the trees is in general agreement with traditional views on evolutionary and systematic relationships of trichomonads. A clear dichotomy is noted between the subfamily Trichomonadinae and the subfamily Tritrichomonadinae. In the latter subfamily, a second division separates the "Tritrichomonas muris-type" species from the "Tritrichomonas augusta-type" ones. Previous evolutionary schemes in which the Monocercomonadidae were regarded as the most "primitive" and the Trichomonadidae as more "evolved" are not in agreement with our molecular data. The emergence of Monocercomonas and Hypotrichomonas at the base of the Tritrichomonas lineage suggests a secondary loss of some cytoskeletal structures, the costa and undulating membrane in these genera. This is corroborated by the early branching position of Trichomitus, which possesses a costa and an undulating membrane and has usually been placed among the Trichomonadidae on the basis of cytological characters. A cladistic analysis was applied to the available morphological characters in order to produce a hierarchical grouping of the taxa reflecting their morphological diversity.     

Control of food spoilage fungi by ethanol

This review discusses the effects of ethanol on the inhibition of growth and germination of fungi and on the inactivation of fungal spores. After a brief survey on the impact of spoilage fungi on the economy and food quality, the major applications of ethanol in controlling fruit decay and extending the shelf-life of food products are reviewed. Many parameters including minimum inhibitory concentration (MIC) and D-values for various moulds are included. The thermodynamic relationship between the liquid phase and the headspace and the mode of action of ethanol on fungi are explained. Due to their promising use as a fumigant, special attention is paid to ethanol vapours.     

A cross validation study of deep brain stimulation targeting: from experts to atlas-based, segmentation-based and automatic registration algorithms

Validation of image registration algorithms is a difficult task and open-ended problem, usually application-dependent. In this paper, we focus on deep brain stimulation (DBS) targeting for the treatment of movement disorders like Parkinson's disease and essential tremor. DBS involves implantation of an electrode deep inside the brain to electrically stimulate specific areas shutting down the disease's symptoms. The subthalamic nucleus (STN) has turned out to be the optimal target for this kind of surgery. Unfortunately, the STN is in general not clearly distinguishable in common medical imaging modalities. Usual techniques to infer its location are the use of anatomical atlases and visible surrounding landmarks. Surgeons have to adjust the electrode intraoperatively using electrophysiological recordings and macrostimulation tests. We constructed a ground truth derived from specific patients whose STNs are clearly visible on magnetic resonance (MR) T2-weighted images. A patient is chosen as atlas both for the right and left sides. Then, by registering each patient with the atlas using different methods, several estimations of the STN location are obtained. Two studies are driven using our proposed validation scheme. First, a comparison between different atlas-based and nonrigid registration algorithms with a evaluation of their performance and usability to locate the STN automatically. Second, a study of which visible surrounding structures influence the STN location. The two studies are cross validated between them and against expert's variability. Using this scheme, we evaluated the expert's ability against the estimation error provided by the tested algorithms and we demonstrated that automatic STN targeting is possible and as accurate as the expert-driven techniques currently used. We also show which structures have to be taken into account to accurately estimate the STN location.     

Segmentation of video sequences and rate control

This paper deals with the relation between segmentation for coding and rate control. The efficiency of a segmentation-based coding scheme heavily relies on this step that defines how many and which regions have to be segmented. In this paper, we show that this problem can be formulated as a rate/distortion problem. The proposed solution not only controls the segmentation, but also defines the coding strategy to be used in each region. Together with the general approach, several simplified versions of the segmentation control are proposed and discussed.     

Numerical simulation of heat and mass transfer during the absorption of hydrogen in a magnesium hydride

This paper presents a numerical work aiming at the prediction of the characteristics of an industrial tank filled with hydrides for hydrogen storage. A validation of the method is given and is followed by the resolution of an example which shows the importance of achieving a three-dimensional modelling for the design of an industrial tank. Finally, recent results obtained on a magnesium hydride laboratory tank are given.     

A physical optics approach to near field RCS computations

The scattered field of a perfectly conducting body treated using a physical optics approach is obtained in near field in either the monostatic or bistatic case. This approach opens new prospects for a broad range of applications in the areas of radar cross section (rcs) and antennas. Numerical examples for simple shapes (circular and rectangular plates) are given. Remarkable phenomena are found and discussed.     

Affaiblissements calculés pour des liaisons Terre-satellite en France

The authors apply a method previously described in the litterature to three consecutive decades, for which the rain intensity at Paris was carefully measured. Computations for 12, 18 and 30 GHz show that the variability of rain attenuation among various decades is quite substantial, does not obey any long term trend, and does not depend upon the frequency. Hence, it is possible to propose an evaluation of prediction uncertainties due to random fluctuations of the rain climate in the long term. Next, specific predictions are carried out for various regions in France, and the possible advantages of high altitude receiving site are estimated. They stress the variability of attenuation with location for 12 and 18 GHz : for a given attenuation margin, outage durations vary by a factor one to ten between various French regions.