Contactless system for dynamic characterisation of microresonators

A cost-effective system for the contactless characterisation of microresonators vibrating in out-of-plane mode is presented. The system provides swept-frequency excitation to the microresonator, detects the mechanical vibrations by a single-point triangulator made by a laser diode and a four-quadrant detector, and exploits synchronous undersampling of the readout signal to derive the frequency response of the microresonator. The system has been prototyped and tested using a piezoelectric bimorph cantilever as the resonator. A displacement resolution of around 100 nm has been obtained in the frequency range of up to some tens of kilohertz.     

SARS-CoV-2 Spike Protein Binding of Glycated Serum Albumin—Its Potential Role in the Pathogenesis of the COVID-19 Clinical Syndromes and Bias towards Individuals with Pre-Diabetes/Type 2 Diabetes and Metabolic Diseases

The immune response to SARS-CoV-2 infection requires antibody recognition of the spike protein. In a study designed to examine the molecular features of anti-spike and anti-nucleocapsid antibodies, patient plasma proteins binding to pre-fusion stabilised complete spike and nucleocapsid proteins were isolated and analysed by matrix-assisted laser desorption ionisation–time of flight (MALDI-ToF) mass spectrometry. Amongst the immunoglobulins, a high affinity for human serum albumin was evident in the anti-spike preparations. Careful mass comparison revealed the preferential capture of advanced glycation end product (AGE) forms of glycated human serum albumin by the pre-fusion spike protein. The ability of bacteria and viruses to surround themselves with serum proteins is a recognised immune evasion and pathogenic process. The preference of SARS-CoV-2 for AGE forms of glycated serum albumin may in part explain the severity and pathology of acute respiratory distress and the bias towards the elderly and those with (pre)diabetic and atherosclerotic/metabolic disease.     

Pheromone Response Inhibitors of the Corn Stalk Borer Sesamia nonagrioides. Biological Evaluation and Toxicology

The behavioral activity of some trifluoromethyl ketones (TFMKs) as inhibitors of the pheromone activity of the corn stalk borer Sesamia nonagrioides (Lef.) (Lepidoptera: Noctuidae) is reported. The most closely-related analogue to the pheromone tested, (Z)-11-hexadecenyl trifluoromethyl ketone (Z11-16:TFMK), elicited a significant decrease in the number of male catches in traps baited with mixtures with the pheromone in 1:1 and 10:1 ratios in comparison to the pheromone alone. The E isomer of the analogue as well as two highly hydrated ketones, 3-octylthio-1,1,1-trifluoropropan-2-one (OTFP) and 1,1-difluoropentadecyl trifluoromethyl ketone, were inactive. Conversely, the saturated TFMKs n-dodecyl trifluoromethyl ketone and, particularly, n-hexadecyl trifluoromethyl ketone induced a synergistic effect when mixed with the synthetic pheromone in 10:1 ratio. However, in a wind tunnel these chemicals did not elicit any differential effect on flying moths attracted to a source containing a 10:1 blend of the analogue and the pheromone. In a dual choice bioassay with two dispensers, containing the pheromone alone, and mixtures of Z11-16:TFMK and the pheromone and separated 5 cm apart, males showed no particular preference for either dispenser. The ketone mixture, however, induced moths to execute erratic flights with frequent crosswind counter-turns and intersections with plume boundaries. The total number of contacts with the source were reduced when a control dispenser was paired with a dispenser containing Z11-16:TFMK and pheromone. Z11-16:TFMK and OTFP showed little toxicity on mice with a LD₅₀ of 1 g/kg after the 6th day of treatment. For comparitive purposes, the major component of the pheromone, Z11-16:Ac, displayed a LD₅₀ of 5 g/kg 6 days after application. Our results provide additional information about the activity of the TFMKs, which might be useful for the utilization of these chemicals in future pest control studies.     

Thermosiphon-based PCR reactor: experiment and modeling

A self-actuated, flow-cycling polymerase chain reaction (PCR) reactor that takes advantage of buoyancy forces to continuously circulate reagents in a closed loop through various thermal zones has been constructed, tested, and modeled. The heating required for the PCR is advantageously used to induce fluid motion without the need for a pump. Flow velocities on the order of millimeters per second are readily attainable. In our preliminary prototype, we measured a cross-sectionally averaged velocity of 2.5 mm/s and a cycle time of 104 s. The flow velocity is nearly independent of the loop's length, making the device readily scalable. Successful amplifications of 700- and 305-bp fragments of Bacillus cereus genomic DNA have been demonstrated. Since the device does not require any moving parts, it is particularly suitable for miniature systems.     

Electrical transport study of La0.7Ca0.3MnO3 using phase - coexistence transport model

In this study, we report the electric transport of La_0.7Ca_0.3MnO₃ prepared by various methods. Based on the fact that the metallic ferromagnetic and insulating paramagnetic clusters simultaneously exist at transition temperature, we applied phase-coexistence transport model to calculate the temperature dependence of the resistivity. This model can explain quantitatively the electrical conductivity of La_0.7Ca_0.3MnO₃ for the whole temperature range studied.     

Predicting the accumulated number of plugged tubes in a steam generator using statistical methodologies

A steam generator (SG) plays a significant role not only with respect to the primary-to-secondary heat transfer but also as a fission product barrier to prevent the release of radionuclides. Tube plugging is an efficient way to avoid releasing radionuclides when SG tubes are severely degraded. However, this remedial action may cause the decrease of SG heat transfer capability, especially in transient or accident conditions. It is therefore crucial for the plant staff to understand the trend of plugged tubes for the SG operation and maintenance. Statistical methodologies are proposed in this paper to predict this trend. The accumulated numbers of SG plugged tubes versus the operation time are predicted using the Weibull and log–normal distributions, which correspond well with the plant measured data from a selected pressurized water reactor (PWR). With the help of these predictions, the accumulated number of SG plugged tubes can be reasonably extrapolated to the 40-year operation lifetime (or even longer than 40 years) of a PWR. This information can assist the plant policymakers to determine whether or when a SG must be replaced.     

Thermal convection in a horizontal, eccentric annulus containing a saturated porous medium—an extended perturbation expansion

Thermal convection in a saturated porous medium confined between two horizontal, isothermal, eccentric cylinders is studied through the use of a regular perturbation expansion in terms of the Darcy-Rayleigh number (R). Terms up to O(R³⁰) are computed. In particular, the Nusselt number (Nu) is expressed as a power series of R for various eccentricities. The above power series has, however, a limited radius of convergence. Various non-linear transformations are employed in order to extend the range of utility of the series. The results are used to demonstrate that the heat transfer in the annulus can be optimized by a proper choice of eccentricity. These results have a bearing on the design of thermal insulators.