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1.
Extracellular enzyme activities and nutrient availability during artificial groundwater recharge 总被引:1,自引:0,他引:1
Reija E. Kolehmainen 《Water research》2009,43(2):405-416
Natural organic matter (NOM) removal is the main objective of artificial groundwater recharge (AGR) for drinking water production and biodegradation plays a substantial role in this process. This study focused on the biodegradation of NOM and nutrient availability for microorganisms in AGR by the determination of extracellular enzyme activities (EEAs) and nutrient concentrations along a flow path in an AGR aquifer (Tuusula Water Works, Finland). Natural groundwater in the same area but outside the influence of recharge was used as a reference. Determination of the specific α-d-glucosidase (α-Glu), β-d-glucosidase (β-Glu), phosphomonoesterase (PME), leucine aminopeptidase (LAP) and acetate esterase (AEST) activities by fluorogenic model substrates revealed major increases in the enzymatic hydrolysis rates in the aquifer within a 10 m distance from the basin. The changes in the EEAs along the flow path occurred simultaneously with decreases in nutrient concentrations. The results support the assumption that the synthesis of extracellular enzymes in aquatic environments is up and down regulated by nutrient availability. The EEAs in the basin sediment and pore water samples (down to 10 cm) were in the same order of magnitude as in the basin water, suggesting similar nutritional conditions. Phosphorus was likely to be the limiting nutrient at this particular AGR site. Furthermore, the extracellular enzymes functioned in a synergistic and cooperative way. 相似文献
2.
Phytosterol‐mediated inhibition of intestinal cholesterol absorption in mice is independent of liver X receptor 下载免费PDF全文
3.
Maija Pöllänen Reija Suihkonen Katja Nevalainen Arto P. Koistinen Mika Suvanto Jyrki Vuorinen Tuula T. Pakkanen 《Polymer Engineering and Science》2013,53(6):1279-1286
The morphological, mechanical, thermal, and tribological properties of high‐density polyethylene (HDPE) composites reinforced with organo‐modified nanoclay (3 and 6 wt%) were studied. A commercial maleic anhydride‐based polymeric compatibilizer (PEgMA) was used to improve the adhesion between the polyethylene and clay. Transmission electron microscopy (TEM) characterization of composites revealed that nanoclay exists mainly in a multilayered structure in the HDPE matrix. Mechanical testing of composites showed that Young's modulus and tensile strength increased with nanoclay content. Coefficients of the linear thermal expansion (CLTE) of HDPE–PEgMA–clay composites were slightly lower in the flow direction than those of HDPE–PEgMA. The tribological properties were measured in dry conditions against a steel counterface. The friction coefficient of the matrix was decreased by the addition of clay. Electron microscopic results suggested that the wear mechanism for HDPE and HDPE composites was mainly adhesive. Clay agglomerates were observed on the worn surfaces of the composites, which may partly explain decreased friction. POLYM. ENG. SCI., 2013. © 2012 Society of Plastics Engineers 相似文献
4.
Reija E. Kolehmainen Ludivine M. Crochet Nina M. Kortelainen J?rg H. Langwaldt Jaakko A. Puhakka 《Canadian Metallurgical Quarterly》2010,136(6):607-615
Artificial groundwater recharge for drinking water production involves infiltration of surface water through sandy soil and its capture into a groundwater aquifer. The transformation of aqueous organic matter is one of the central issues in this process. The purpose of this work was to assess the potential of indigenous microorganisms in the source water to contribute in the aqueous organic matter biodegradation. For this purpose, microorganisms were enriched from the source water in a fluidized-bed reactor (FBR) and used for kinetic studies on biodegradation of organic matter at ambient temperature range. Lake water (total organic carbon 5.8?mg?L?1) was continuously fed to the FBR containing porous carrier material to support biomass retention. In the inlet and outlet water there were on average 21±6 and 13±5×105?cells?mL?1, respectively. Biofilm accumulation (as volatile solids) reached 13.1?mg?g?1 dw carrier. In the continuous-flow mode and the batch tests, the highest oxygen consumption rate appeared in the summer, followed by the fall, spring, and winter. At low temperatures, the biodegradation of aqueous organic matter was relatively rapid initially for labile fractions followed by a slower phase for refractory fractions. The average temperature coefficient (Q10) in the system was 2.3 illustrating a strong temperature dependency of oxygen consumption. The isotopic analysis of dissolved inorganic carbon δ13CDIC analysis revealed 27 and 69% mineralizations of dissolved organic carbon at 23 and 6°C over 65 and 630 min, respectively. These results can be used to construct additional input parameters in modeling applications of artificial groundwater recharge process. The biological component especially, i.e., the biodegradation, is difficult to predict for on-site applications without experimental proof and thus the interpretation in this study will help formulate design predictions for the process. 相似文献
5.
Artificial groundwater recharge (AGR) is used in the drinking water industry to supplement groundwater resources and to minimise the use of chemicals in water treatment. This study analysed the spatial and temporal changes of microbial communities in AGR using two test systems: a nutrient-amended fluidized-bed reactor (FBR) and a sand column. Structural changes in the feed lake water (Lake Roine), FBR, and sand column bacterial communities were determined by denaturing gradient gel electrophoresis (DGGE) and the length heterogeneity analysis of amplified 16S rRNA genes (LH-PCR). Two clone libraries were created to link the LH-PCR results to the dominant bacterial groups. The lake water bacterial community was relatively stable, with three bands dominating in all LH-PCR products. The most dominant fragment accounted for up to 72% and was derived from Actinobacteria. Based on the clone libraries and LH-PCR data, Actinobacteria also dominated in the unattached bacterial community of the FBR, whereas several Proteobacterial groups were more abundant on the FBR carrier particles. In the stabilised AGR system a major change in the community structure of the lake water bacteria took place during passage within the first 0.6 m in the sand column as the community composition shifted from Actinobacteria-dominated populations to a diverse, mainly Proteobacterial communities. Concurrently, most of the dissolved organic carbon (DOC) was removed at this stage. In summary, the study showed that the make-up of microbial communities in experimental AGR systems responded to changes in their environment. LH-PCR showed potential as a method to determine microbial community dynamics in long-term studies at real-scale AGR sites. This is the first step to provide data on microbial community dynamics in AGR for drinking water production. 相似文献
6.
This study evaluated the removal of natural organic matter (NOM) and structural changes in the microbial community during infiltration of humic lake water at three artificial groundwater recharge (AGR) sites in Finland. The three sites were at waterworks in H?meenlinna, Jyv?skyl? and Tuusula, sites A, B and C, respectively. Site A used groundwater recharge by both basin and sprinkling infiltration, site B used only sprinkling infiltration, and site C used only basin infiltration. Reductions of total organic carbon at sites A, B and C were 91%, 84% and 74%, respectively, in the winter, and 88%, 77% and 73%, respectively, in the summer. The Finnish national recommended value of 2 mg/l for TOC was achieved at all sites and the TOC of natural groundwater at site C was much lower, at 0.6 mg/l. Large molecular fractions of NOM were removed more efficiently than the smaller ones. Total amount of DAPI-stained cells decreased during infiltration at sites A, B and C in winter by 94%, 94% and 75% and in summer by 96%, 97% and 94%, respectively. Bacterial communities in raw waters and extracted groundwaters were diverse with changes occurring during infiltration, which was shown by DNA extraction followed by PCR of 16S rRNA genes and denaturing gradient gel electrophoresis (DGGE) fingerprinting. While the natural groundwater microbial community was diverse, it was different from that of the extracted groundwater in the AGR area. Simultaneous organic carbon removal and the decrease of bacterial counts during infiltration indicated biodegradation. In addition, the changing DGGE profiles during the process of infiltration, demonstrated that changing environmental conditions were reflected by changes in bacterial community composition. 相似文献
7.
After-use solutions for natural stone quarries were studied for planning possible ways of using an abandoned (some other word might be better) quarry area. Characteristics of a natural stone quarry include physically stable quarry faces and benches created by quarrying, a water pond at the bottom of the quarry, and piles of leftover stone material. These features can be utilized as central elements in the quarry after-use. Scuba diving, climbing, and forestry are traditional and affordable afteruse alternatives, while fish or crab farming, rock building, storing, and amusement parks are more challenging solutions. The quarry can also be used for culture, learning, and research purposes. 相似文献
8.
Reija Suihkonen Katja Nevalainen Olli Orell Mari Honkanen Longcheng Tang Hui Zhang Zhong Zhang Jyrki Vuorinen 《Journal of Materials Science》2012,47(3):1480-1488
Magnesium hydroxide (MDH) particles are often used as fillers to improve the flame retardancy of polymers. However, achieving
the balance between the enhanced fire resistance and reduced mechanical properties, especially toughness, is still a challenge
to the composite community. In this study, the effect of the particle size and silane surface modification of MDH particles
on the flame retardant, thermal, and mechanical properties of epoxy was studied. Both nano- and micro-sized MDH particles
were modified by a silanization reaction with γ-aminopropyltrietoxysilane in an aqueous solution and filled into epoxy matrix
by a high-shear mixer and a three-roll mill. Results show that nano-MDH filled epoxy composites yielded better mechanical
properties than their micro-MDH filled counterparts. Furthermore, the adhesion between nano-sized MDH and the matrix was improved
by the silane surface modification. When comparing the flame retardant properties, enhancements in heat release rates and
total heat released were observed for MDH filled epoxy composites. 相似文献
9.
A. Lankinen T. Lang S. Suihkonen O. Svensk A. Säynätjoki T. O. Tuomi P. J. McNally M. Odnoblyudov V. Bougrov A. N. Danilewsky P. Bergman R. Simon 《Journal of Materials Science: Materials in Electronics》2008,19(2):143-148
GaN layers grown by metal organic vapour phase epitaxy on sapphire were imaged by synchrotron radiation X-ray topography.
The threading dislocations could not be resolved in the topographs due to their high density, but a smaller density of about
105 cm−2 defects were seen in the interface between GaN and sapphire by utilizing large-area back-reflection topography for the sapphire
substrates. The misfit dislocation images in the topographs form a well-resolved cellular network, in which the average cell
size is roughly 30 μm. Different cell shapes in the misfit dislocation networks are observed on different samples. Also, images
of small-angle grains of similar size were found in transmission section topographs of the GaN layers. 相似文献