High‐Pressure Thermal Sterilization: Food Safety and Food Quality of Baby Food Puree

The benefits that high‐pressure thermal sterilization offers as an emerging technology could be used to produce a better overall food quality. Due to shorter dwell times and lower thermal load applied to the product in comparison to the thermal retorting, lower numbers and quantities of unwanted food processing contaminants (FPCs), for example, furan, acrylamide, HMF, and MCPD‐esters could be formed. Two spore strains were used to test the technique; Geobacillus stearothermophilus and Bacillus amyloliquefaciens, over the temperature range 90 to 121 °C at 600 MPa. The treatments were carried out in baby food puree and ACES‐buffer. The treatments at 90 and 105 °C showed that G. stearothermophilus is more pressure‐sensitive than B. amyloliquefaciens. The formation of FPCs was monitored during the sterilization process and compared to the amounts found in retorted samples of the same food. The amounts of furan could be reduced between 81% to 96% in comparison to retorting for the tested temperature pressure combination even at sterilization conditions of F₀‐value in 7 min.     

Identifikation NSO-heterocyclischer Prioritärsubstanzen zur Erkundung und Überwachung Teeröl-kontaminierter Standorte

The German Federal Soil Protection and Contaminated Sites Ordinance (BBodSchV) requires the monitoring of NSO-heterocyclic hydrocarbons without specifying the extent of such analysis. An assessment procedure for the identification of NSO-heterocyclic priority substances was thus created within the “KORA” project (“Natural Attenuation: Retention and Degradation Processes Reducing Contaminations in Groundwater and Soil”) funded by the German Federal Ministry of Education and Research (BMBF). This assessment includes physicochemical properties, biodegradability, toxicity, and groundwater propagation of these substances at creosote-contaminated sites. In addition to unsubstituted compounds, the derived list of 20 priority NSO-heterocycles also contains substituted derivatives as well as relevant transformation products.     

Conversion of 3-chlorocatechol by various catechol 2,3-dioxygenases and sequence analysis of the chlorocatechol dioxygenase region of Pseudomonas putida GJ31

Pseudomonas putida GJ31 contains an unusual catechol 2,3-dioxygenase that converts 3-chlorocatechol and 3-methylcatechol, which enables the organism to use both chloroaromatics and methylaromatics for growth. A 3.1-kb region of genomic DNA of strain GJ31 containing the gene for this chlorocatechol 2,3-dioxygenase (cbzE) was cloned and sequenced. The cbzE gene appeared to be plasmid localized and was found in a region that also harbors genes encoding a transposase, a ferredoxin that was homologous to XylT, an open reading frame with similarity to a protein of a meta-cleavage pathway with unknown function, and a 2-hydroxymuconic semialdehyde dehydrogenase. CbzE was most similar to catechol 2,3-dioxygenases of the 2.C subfamily of type 1 extradiol dioxygenases (L. D. Eltis and J. T. Bolin, J. Bacteriol. 178:5930-5937, 1996). The substrate range and turnover capacity with 3-chlorocatechol were determined for CbzE and four related catechol 2,3-dioxygenases. The results showed that CbzE was the only enzyme that could productively convert 3-chlorocatechol. Besides, CbzE was less susceptible to inactivation by methylated catechols. Hybrid enzymes that were made of CzbE and the catechol 2, 3-dioxygenase of P. putida UCC2 (TdnC) showed that the resistance of CbzE to suicide inactivation and its substrate specificity were mainly determined by the C-terminal region of the protein.     

Factors influencing the growth of Salmonella during sprouting of naturally contaminated alfalfa seeds

In this study, the factors that affect Salmonella growth during sprouting of naturally contaminated alfalfa seeds associated with two previous outbreaks of salmonellosis were examined. A minidrum sprouter equipped with automatic irrigation and rotation systems was built to allow sprouting to be conducted under conditions similar to those used commercially. The growth of Salmonella during sprouting in the minidrum was compared with that observed in sprouts grown in glass jars under conditions commonly used at home. The level of Salmonella increased by as much as 4 log units after 48 h of sprouting in jars but remained constant during the entire sprouting period in the minidrum. The effect of temperature and irrigation frequency on Salmonella growth was examined. Increasing the sprouting temperature from 20 to 30 degrees C increased the Salmonella counts by as much as 2 log units on sprouts grown both in the minidrum and in the glass jars. Decreasing the irrigation frequency from every 20 min to every 2 h during sprouting in the minidrum or from every 4 h to every 24 h during sprouting in the glass jars resulted in an approximately 2-log increase in Salmonella counts. The levels of total aerobic mesophilic bacteria, coliforms, and Salmonella in spent irrigation water closely reflected those found in sprouts, confirming that monitoring of spent irrigation water is a good way to monitor pathogen levels during sprouting.     

Efficiency of Light Outcoupling Structures in Organic Light‐Emitting Diodes: 2D TiO2 Array as a Model System

Organic light‐emitting diodes (OLEDs) are widely used in research and are established in the industry. The building block nature of organic compounds enables a vast variety of materials. On top of that, there exist many strategies to improve the light outcoupling of OLEDs making a direct comparison of outcoupling technologies difficult. Here, a novel approach is introduced for the evaluation of light outcoupling structures. The new defined “efficiency of light outcoupling structures” (ELOS) clearly determines the effectiveness of the light outcoupling structure by weighting the experimental efficiency enhancement over the theoretical outcoupling gain. It neither depends on cavity design nor on the chosen organic material. The methodology is illustrated for red phosphorescent OLEDs comprising internal and external light outcoupling structures. Assumptions and further uses are discussed with respect to experimental and theoretical handling. In addition, the ELOS is calculated for various outcoupling techniques from literature to demonstrate the universality. Finally, most suitable reference OLEDs are discussed for application of light outcoupling structures. The presented approach enables new possibilities for studying light outcoupling structures and improves their comparability in a highly material‐driven research field.     

Design and analysis of SIC: a provably timing-predictable pipelined processor core

We introduce the strictly in-order core (SIC), a timing-predictable pipelined processor core. SIC is provably timing compositional and free of timing anomalies. This enables precise and efficient worst-case execution time (WCET) and multi-core timing analysis. SIC’s key underlying property is the monotonicity of its transition relation w.r.t. a natural partial order on its microarchitectural states. This monotonicity is achieved by carefully eliminating some of the dependencies between consecutive instructions from a standard in-order pipeline design. We present a formal proof framework based on satisfiability modulo theories that is able to automatically verify SIC’s timing predictability. SIC preserves most of the benefits of pipelining: it is only about 6–7% slower than a conventional non-strict in-order pipelined processor. Its timing predictability enables orders-of-magnitude faster WCET and multi-core timing analysis than conventional designs.

     

Electrothermal Tristability Causes Sudden Burn-In Phenomena in Organic LEDs

Organic light-emitting diodes (OLEDs) have been established as a mature display pixel technology. While introducing the same technology in a large-area form factor to general lighting and signage applications, some key questions remain unanswered. Under high-brightness conditions, OLED panels were reported to exhibit nonlinear electrothermal behavior causing lateral brightness inhomogeneities and even regions of switched-back luminance. Also, the physical understanding of sudden device failure and burn-ins is still rudimentary. A safe and stable operation of lighting tiles, therefore, requires an in-depth understanding of these physical phenomena. Here, it is shown that the electrothermal treatment of thin-film devices allows grasping the underlying physics. Configurations of OLEDs with different lateral dimensions are studied as a role model and it is reported that devices exceeding a certain panel size develop three stable, self heating-induced operating branches. Switching between them causes the sudden formation of dark spots in devices without any preexisting inhomogeneities. A current-stabilized operation mode is commonly used in the lighting industry, as it ensures degradation-induced voltage adjustments. Here, it is demonstrated that a tristable operation always leads to destructive switching, independent of applying constant currents or voltages. With this new understanding of the effects at high operation brightness, it will be possible to adjust driving schemes accordingly, design more resilient system integrations, and develop additional failure mitigation strategies.     

Design and synthesis of metal-carboxylate frameworks with permanent microporosity

Metal-carboxylate clusters are ideally suited as secondary building units (SBU) for the assembly of extended open frameworks. Examples extracted from the chemistry of the multidentate building block 1,4-benzenedicarboxylate (BDC) show that Zn(BDC) clusters lead to the formation of rigid, stable and truly microporous metal-carboxylate networks. Gas sorption isotherms measured for this class of materials show type (I) gas uptake that is analogous to those observed for zeolites and molecular sieves. This revised version was published online in August 2006 with corrections to the Cover Date.     

Growth of Salmonella during sprouting of alfalfa seeds associated with salmonellosis outbreaks

Growth of Salmonella was assessed during sprouting of naturally contaminated alfalfa seeds associated with two outbreaks of salmonellosis. Salmonella was determined daily in sprouts and sprout rinse water samples by a three-tube most probable number (MPN) procedure and a commercial enzyme immunoassay (EIA). Growth of Salmonella in the sprouts was reflected in the rinse water, and the MPNs of the two samples were generally in agreement within approximately 1 log. The results from EIA testing of sprouts and water samples were also in agreement. The pathogen was present in the seed at less than 1 MPN/g, and it increased in number to maximum population levels of 102 to 10(3) MPN/g in one seed lot and 10(2) to 10(4) MPN/ g in the other seed lot. Maximum populations of the pathogen were apparent by day 2 of sprouting. These results show the ability of the pathogen to grow to detectable levels during the sprouting process, and they provide support for the recommendation to test the sprout water for the presence of pathogens 48 h after starting seed sprouting. The effectiveness of a 10-min, 20,0000-microg/ml (ppm) calcium hypochlorite treatment of the outbreak-associated seeds was studied. For both seed lots, the hypochlorite treatment caused a reduction, but not elimination, of Salmonella contamination in the finished sprouts. These results confirm the need to test each production batch for the presence of pathogens, even after 20,000 microg/ml (ppm) hypochlorite treatment of seeds, so that contaminated product is not distributed.