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1.
Ilaria Fraudentali Renato A. Rodrigues-Pousada Riccardo Angelini Sandip A. Ghuge Alessandra Cona 《International journal of molecular sciences》2021,22(10)
Polyamines are ubiquitous, low-molecular-weight aliphatic compounds, present in living organisms and essential for cell growth and differentiation. Copper amine oxidases (CuAOs) oxidize polyamines to aminoaldehydes releasing ammonium and hydrogen peroxide, which participates in the complex network of reactive oxygen species acting as signaling molecules involved in responses to biotic and abiotic stresses. CuAOs have been identified and characterized in different plant species, but the most extensive study on a CuAO gene family has been carried out in Arabidopsis thaliana. Growing attention has been devoted in the last years to the investigation of the CuAO expression pattern during development and in response to an array of stress and stress-related hormones, events in which recent studies have highlighted CuAOs to play a key role by modulation of a multilevel phenotypic plasticity expression. In this review, the attention will be focused on the involvement of different AtCuAOs in the IAA/JA/ABA signal transduction pathways which mediate stress-induced phenotypic plasticity events. 相似文献
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3.
Patricio Toro Raúl Quijada Omar Murillo Mehrdad Yazdani‐Pedram 《Polymer International》2005,54(4):730-734
The mechanical, morphological behavior and water absorption characteristics of polypropylene (PP) and silica, or PP and rice‐husk, composites have been studied. The silica used in this study as filler was a commercial type produced from soluble glass or rice husks. The compatibilizing effect of PP grafted with monomethyl itaconate (PP‐g‐MMI) and/or with vinyltriethoxysilane (PP‐g‐VTES) as polar monomers on the mechanical properties and water absorption was also investigated. In general, a high loading of the studied fillers in the polymer matrix increases the stiffness and the water absorption capacity. This effect is more noticeable in the tensile modulus of the PP/silica composite with PP‐g‐VTES as compatibilizer. However, the increase of the rice‐husk charge as a natural filler in the PP matrix decreases the stiffness, and in the presence of PP‐g‐MMI as compatibilizer in PP/rice‐husk, the tensile modulus and water absorption of the composite were improved. The better adhesion and phase continuity in the PP/silica and PP/rice‐husk composites with different compatibilizers was confirmed by the morphological study. Copyright © 2004 Society of Chemical Industry 相似文献
4.
Renato Cataluña Rosângela da Silva Eliana Weber de Menezes Ricardo Boeira Ivanov 《Fuel》2008,87(15-16):3362-3368
Oxygenated fuels increase fuel consumption due to their low enthalpy of combustion; however, their high antiknock index renders them suitable for use in engines with a high compression rate, increasing their thermal yield. This study evaluated the performance of biorenewable oxygenated fuels (ethanol and isoamyl alcohol) and partially renewable fuels (ETBE: ethyl tert-butyl ether, TAEE: tert-amyl ethyl ether and di-TAE: di-tert-amyl ether) with high degree of purity and in mixtures with automotive gasoline, based on tests with Otto cycle engines. Among the oxygenated fuels evaluated here, di-TAE was found to present the best characteristics of performance, both individually and in mixtures with gasoline. 相似文献
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6.
Renato Maia Renato Cerqueira Clarisse Sieckenius de Souza Tomás Guisasola-Gorham 《Empirical Software Engineering》2012,17(3):166-199
Today middleware is much more powerful, more reliable and faster than it used to be. Nevertheless, for the application developer,
the complexity of using middleware platforms has increased accordingly. The volume and variety of application contexts that
current middleware technologies have to support require that developers be able to anticipate the widest possible range of
execution environments, desired and undesired effects of different programming strategies, handling procedures for runtime
errors, and so on. This paper shows how a generic framework designed to evaluate the usability of notations (the Cognitive Dimensions of Notations Framework, or CDN) has been instantiated and used to analyze the cognitive
challenges involved in adapting middleware platforms. This human-centric perspective allowed us to achieve novel results compared
to existing middleware evaluation research, typically centered around system performance metrics. The focus of our study is
on the process of adapting middleware implementations, rather than in the end product of this activity. Our main contributions
are twofold. First, we describe a qualitative CDN-based method to analyze the cognitive effort made by programmers while adapting
middleware implementations. And second, we show how two platforms designed for flexibility have been compared, suggesting
that certain programming language design features might be particularly helpful for developers. 相似文献
7.
Jose Saldana Julián Fernández-Navajas José Ruiz-Mas Jenifer Murillo Eduardo Viruete Navarro José I. Aznar 《Computer Networks》2012,56(7):1893-1919
This work presents a study of RTP multiplexing schemes, which are compared with the normal use of RTP, in terms of experienced quality. Bandwidth saving, latency and packet loss for different options are studied, and some tests of Voice over IP (VoIP) traffic are carried out in order to compare the quality obtained using different implementations of the router buffer. Voice quality is calculated using ITU R-factor, which is a widely accepted quality estimator. The tests show the bandwidth savings of multiplexing, and also the importance of packet size for certain buffers, as latency and packet loss may be affected. The customer’s experience improvement is measured, showing that the use of multiplexing can be interesting in some scenarios, like an enterprise with different offices connected via the Internet. The system is also tested using different numbers of samples per packet, and the distribution of the flows into different tunnels is found to be an important factor in order to achieve an optimal perceived quality for each kind of buffer. Grouping all the flows into a single tunnel will not always be the best solution, as the increase of the number of flows does not improve bandwidth efficiency indefinitely. If the buffer penalizes big packets, it will be better to group the flows into a number of tunnels. The router processing capacity has to be taken into account too, as the limit of packets per second it can manage must not be exceeded. The obtained results show that multiplexing is a good way to improve customer’s experience of VoIP in scenarios where many RTP flows share the same path. 相似文献
8.
Rudolf E. Schmitt Johann Haas und Renato Amadò 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1988,187(2):121-124
Zusammenfassung Zur Bestimmung biogener Amine aus Schlachtgeflügel wird ein einfach durchzuführendes Analysenverfahren beschrieben. Die Amine wurden mit 0,6m-Perchlorsäure extrahiert, nach Dansylierung auf einer RP-18 Säule aufgetrennt und bei 254 nm detektiert. Innerhalb von 7 min konnte eine gute Auftrennung von 8 Aminen erreicht werden. Zur quantitativen Erfassung von Spermin und Spermidin mußte der Extrakt zusätzlich über Amberlite CG 50 gereinigt werden. Die Wiederfindungsraten betrugen 82% bis 96%, die Nachweisgrenzen 0,2 bis 0,5 g pro g Hühnerhaut. Bei Schlachtgeflügel eignen sich Putrescin und Cadaverin gut als Leitsubstanzen für den einsetzenden Verderb, da sie bereits ab Gesamtkoloniezahlen von etwa 105 pro cm2 nachweisbar sind, und ihre Konzentration mit zunehmendem Verderb rasch ansteigen.
Determination of biogenic amines by RP-HPLC for monitoring microbial spoilage of poultry
Summary A simple high-performance liquid chromatographic (HPLC) analysis is described for the determination of biogenic amines in broiler carcasses. The clean-up procedure consists of an extraction with 0.6M-perchloric acid, formation of dansyl derivatives, separation by a RP-18 column and UV detection at 254 nm. Within 7 min eight amines could be separated. The quantitative determination of spermidine and spermine requires an additional ion-exchange clean-up with Amberlite CG 50 after the extraction. This procedure gives recoveries of 82%–96% with detection limits of 0.2–0.5 g/g of broiler skin. Putrescine and cadaverine are good indicators for the onset of spoilage of poultry carcasses, since both amines could be detected at total colony counts of 105 cfu/cm2 and their concentration increases rapidly with advancing decomposition.相似文献
9.
Rafael S. Pinto João P. Serra João C. Barbosa Renato Gonçalves Maria M. Silva Senentxu Lanceros-Méndez Carlos M. Costa 《大分子材料与工程》2021,306(11):2100372
Considering the high levels of materials used in the fields of electronics and energy storage systems, it is increasingly necessary to take into consideration environmental impact. Thus, it is important to develop devices based on environmentally friendlier materials and/or processes, such as additive manufacturing techniques. In this work, poly(vinylidene fluoride) (PVDF) and poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) are prepared by direct-ink-writing (DIW) by varying solvent evaporation temperature and fill density percentage. Different morphologies for both polymers are obtained, including dense films and porous membranes, as well as different electroactive β-phase content, thermal and mechanical properties. The dielectric constant and piezoelectric d33 coefficient for dense films reaches up to 16 at 1 kHz and 4 pC N−1, respectively for PVDF-HFP with a fill density of 80 and a solvent evaporation temperature of 50 °C. Porous structures are developed for battery separator membranes in lithium-ion batteries, with a highest ionic conductivity value of 3.8 mS cm−1 for the PVDF-HFP sample prepared with a fill density of 100 and a solvent evaporation temperature of 25 °C, the sample showing an excellent cycling performance. It is demonstrated that electroactive films and membranes can be prepared by direct-ink writing suitable for sensors/actuators and energy storage systems. 相似文献
10.
Z. Benzo T. Zoltan M. Murillo M. Quintal J. Salas E. Marcano C. Gómez 《Journal of the American Oil Chemists' Society》2006,83(5):401-405
An emulsion-based method was developed as an alternative for the introduction of oil-based samples into a graphite furnace
to produce a more rapid, precise, and accurate method of analysis. The combination of emulsion sampling and electrothermal
atomization for atomic absorption spectroscopy was developed and then applied to the determination of Ni and manganese in
neem oil from Venezuela. The optimal concentration of neem oil in the emulsion was 30 and 4% for Ni and Mn, respectively,
based on the metal concentration of the neem oil. The stability of the emulsion as a function of time was evaluated. The use
of aqueous calibration solutions and the accuracy attained using this method make it attractive for the analysis of oil samples.
The metal recovery was between 97 and 101%. Based on the emulsion method, the neem oil samples had 1.39 and 0.21 mg of Mn
and Ni per kg of oil, respectively. The wet digestion method indicated a Mn and Ni concentration in the neem oil sample of
1.42 and 0.24 mg/kg, respectively. 相似文献