全文获取类型
收费全文 | 1964篇 |
免费 | 20篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 4篇 |
化学工业 | 257篇 |
金属工艺 | 30篇 |
机械仪表 | 55篇 |
建筑科学 | 8篇 |
能源动力 | 26篇 |
轻工业 | 98篇 |
水利工程 | 3篇 |
石油天然气 | 1篇 |
无线电 | 112篇 |
一般工业技术 | 168篇 |
冶金工业 | 1152篇 |
原子能技术 | 10篇 |
自动化技术 | 62篇 |
出版年
2023年 | 4篇 |
2022年 | 8篇 |
2021年 | 19篇 |
2020年 | 8篇 |
2019年 | 5篇 |
2018年 | 10篇 |
2017年 | 10篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 28篇 |
2013年 | 34篇 |
2012年 | 40篇 |
2011年 | 42篇 |
2010年 | 39篇 |
2009年 | 43篇 |
2008年 | 44篇 |
2007年 | 37篇 |
2006年 | 21篇 |
2005年 | 32篇 |
2004年 | 42篇 |
2003年 | 44篇 |
2002年 | 45篇 |
2001年 | 29篇 |
2000年 | 44篇 |
1999年 | 55篇 |
1998年 | 364篇 |
1997年 | 180篇 |
1996年 | 151篇 |
1995年 | 90篇 |
1994年 | 84篇 |
1993年 | 81篇 |
1992年 | 13篇 |
1991年 | 28篇 |
1990年 | 25篇 |
1989年 | 22篇 |
1988年 | 25篇 |
1987年 | 16篇 |
1986年 | 17篇 |
1985年 | 14篇 |
1984年 | 8篇 |
1982年 | 14篇 |
1981年 | 10篇 |
1980年 | 13篇 |
1978年 | 9篇 |
1977年 | 36篇 |
1976年 | 54篇 |
1975年 | 6篇 |
1974年 | 5篇 |
1973年 | 6篇 |
1970年 | 4篇 |
排序方式: 共有1986条查询结果,搜索用时 15 毫秒
1.
2.
MC Jacobson E Franssen BD Birt MJ Davidson RW Gilbert 《Canadian Metallurgical Quarterly》1997,26(3):171-179
The aim of this study was to investigate the effect of the absence of elongate spermatids (ES) from the rat seminiferous epithelium on the quantitative secretion and synthesis of the three major Sertoli cell secretory proteins--SGP-1, SGP-2 and CP-2. Seminiferous tubules (ST) were isolated (a) from normal 28-day-old rats, in which the most mature germ cell type is the round spermatid, (b) from normal adult rats at stages IX-XIV of the spermatogenic cycle, i.e. after spermiation, or at stages I-V and VI-VIII, when ES are still attached to the Sertoli cell, and (c) at stages VI-VIII from normal adult rats and from rats treated with methoxyacetic acid (MAA) in order to specifically deplete ES at these stages. Two-dimensional SDS PAGE combined with computerized image analysis was used to analyse 35S-methionine-labelled intracellular and secreted proteins. In the case of SGP-1 and SGP-2, almost all of the protein synthesized by ST was secreted. The total amount of both SGP-1 and CP-2 secreted by unstaged ST from immature rats was significantly lower than that secreted by unstaged ST from adult rats. The total amount of SGP-1 and CP-2 secreted by adult ST at stages IX-XIV of the spermatogenic cycle also declined dramatically compared to ST at earlier stages. The proportion of the total CP-2 synthesized by ST which was secreted also declined in all situations in which ES were absent from the seminiferous epithelium. The synthesis of only SGP-2 was changed by ES depletion from ST at stages VI-VIII, which was almost doubled compared to synthesis of this protein by ST from control rats. Our results suggest strongly that the secretion of SGP-1 and SGP-2 is via the constitutive pathway, and that regulation of these two proteins by ES is at the level of protein synthesis. In contrast, the regulation of CP-2 by ES is predominantly at the level of secretion, suggesting that this protein is secreted via a regulated pathway. Our findings add to the evidence showing that ES play a major role in the regulation of Sertoli cell function. 相似文献
3.
JW Rhee 《The Journal of communication》1997,47(3):26-48
This study examines how news frames in campaign coverage affect individuals' interpretation of campaigns. Framing effects are conceptualized in terms of the process by which textual features in news stories set limits on knowledge use in the construction of a discourse model - a mental model constructed through the interaction between news frames and the interpreter's social knowledge. Examining message structures in campaign news stories led to identifying two major frames in campaign coverage: strategy and issue. Narrative data collected from two field experiments were analyzed. The findings suggest that both strategy-framed and issue-framed print news stories are effective in influencing campaign interpretation. 相似文献
4.
5.
Low dielectric poly[methylsilsesquioxane‐ran‐trifluoropropylsilsesquioxane‐ran‐(2,4,6,8‐tetramethyl‐2,4,6,8‐tetraethylenecyclotetrasiloxane)silsesquioxane]s {P[M‐ran‐TFP‐ran‐(TCS)]SSQs} having various compositions were synthesized using trifluoropropyl trimethoxysilane, methyl trimethoxysilane and 2,4,6,8‐tetramethyl‐2,4,6,8‐tetra(trimethoxysilylethyl)cyclotetrasiloxane. The chemical composition of the polymers and the content of SiOH end‐groups were controlled by adjusting the reaction conditions, and they were characterized by 1H‐NMR. The thermally decomposable trifluoropropyl groups on the P[M‐ran‐TFP‐ran‐(TCS)]SSQ backbone and heptakis(2,3,6‐tri‐O‐methyl)‐β‐cyclodextrin (CD) were employed as pore generators. The dielectric constants of the porous CD/P[M‐ran‐TFP‐ran‐(TCS)]SSQ films were in the range 2.0–2.7 (at 100 kHz) depending on the concentration of the porogens, and showed no change over 4 days under aqueous conditions. The pore size of the films showed a bimodal distribution, with diameters of ca 0.5–1.0 nm for those originating from the trifluoropropyl groups and 1.7 nm from the CD. The elastic modulus and hardness of the 30 vol% CD‐blended film with a dielectric constant of 2.26 were 2.40 and 0.38 GPa, respectively, as determined by a nanoindenter. Copyright © 2005 Society of Chemical Industry 相似文献
6.
BD Shenstone A Mahmoud R Woodward D Elvins R Palmer EF Ring AK Bhalla 《Canadian Metallurgical Quarterly》1994,33(6):541-545
A prospective longitudinal study of patients with early RA was performed to examine the influence of disease duration, disease activity and physical activity on bone loss. Sixty-seven patients with non-steroid treated RA of less than 5 yr duration, including 16 patients with disease duration less than 6 months, had BMD measurements of the femoral neck and the lumbar spine over a 12-month period using dual energy X-ray absorptiometry. The BMD changes were compared with values from 72 control patients and were also correlated with serial measurements of disease activity (measured by the Stoke Index) and disability [measured by the Health Assessment Questionnaire (HAQ) score], at 3-monthly intervals over the 12-month period. No significant differences in BMD changes were found between RA patients and controls overall. Patients with disease duration of less than 6 months had significantly greater loss of BMD at the femoral neck (-3.9%, S.E.M. 1.5) than the remainder of the cohort (-0.2%, S.E.M. 0.7) (P = 0.02) and controls (-0.8%, S.E.M. 0.6). Lumbar spine BMD changes correlated with the initial Stoke Index (Rs-0.373, P = 0.01) but not mean Stoke Indices. There was no correlation of BMD changes with age or HAQ scores. These findings suggest that significant bone loss occurs within the first few months of disease in patients with RA. 相似文献
7.
AA Bakova BD Brondz OA Fedorkina VA Gusev AI Gordienko IuS Krivoshein IuL Krivorutchenko TG Prokopenko IA Popov 《Canadian Metallurgical Quarterly》1994,39(4):187-189
The possibilities of using influenza A (Leningrad) 385/80 (H3N2) virus matrix protein-specific FITC-labeled D8 monoclonal antibodies in immunofluorescence assays were investigated. The virus antigen accumulation was detected in chorioallantoic cells of chick embryos. Exhibiting the type-specific properties, the fluorescent antibodies stain the perinuclear space, cytoplasmic membrane, and granular structures in the cytoplasm of infected cells. The haemagglutination test tires in the corresponding specimens were at least 1:16. 相似文献
8.
An on-line, steam distillation/purge and trap gas chromatographic procedure is described for determination of halogenated analytes in foods and beverages. Recoveries were generally >80% (versus aqueous standards) from vegetable oil, flour, root beer, cream (10% butter fat), and milk spiked at 1-3 micrograms/kg for each of the 32 analytes studied. Analytes ranged in volatility from vinyl chloride to 1,2,3,4-tetrachlorobenzene. Repeatabilities from aqueous standards were <10% for most analytes. For a 1 g food sample, method detection limits ranged from 0.02 to 0.2 micrograms/kg for the 32 analytes. Reduced recoveries for less volatile analytes, however, occurred when steam-distillable, nonpolar food components were carried to the sparger. This effect was observed for citrus beverages containing steam-volatile limonene, roasted and ground coffees, and some salad dressings. The method was applied to a variety of foods. 相似文献
9.
Identification of a specific biomolecular target appropriately sensitive to a wide array of anesthetics has been elusive. At concentrations close to their respective ED50's for anesthesia in man or other species, 18 compounds, differing in potencies up to 66,000 fold, inhibited cytochrome P450 mediated metabolism of aminopyrine, a synthetic substrate, and arachidonic acid (AA), an endogenous substrate, in isolated liver microsomes. There was a highly significant correlation for both substrates between the absolute concentrations required for anesthesia (EC50) and for inhibition of P450 activity (Ki or IC50). The mean Ki/EC50 ratio was 0.97 for inhibition of aminopyrine demethylase. The mean IC50/EC50 ratios were 0.42 and 0.64 for inhibition of two AA-derived products and 2.8 for a third; a mean ratio of 1.4 for inhibition of overall AA metabolism suggests interaction of general anesthetics with a composite of P450 isozymes. The universal cytochrome P450 monooxygenases, in conjunction with other lipid oxygenases (cyclooxygenases and lipoxygenases) participate in the second messenger AA cascade. In nerve cells the sensitivity of these enzymes to hydrophobic neurodepressant drugs may underlie the state of general anesthesia: reversible disruption of intracellular and intercellular signalling without impairment of enzymes vital to cell respiration. 相似文献
10.
F van Rhee RM Szydlo J Hermans A Devergie F Frassoni W Arcese T de Witte HJ Kolb D Niederwiser N Jacobsen G Gahrton G Bandini E Carreras A Bacigalupo M Michallet T Ruutu J Reiffers JM Goldman J Apperley A Gratwohl 《Canadian Metallurgical Quarterly》1997,20(7):553-560
The purpose of this study was to determine the long-term results of allogeneic bone marrow transplantation for chronic myeloid leukemia. A retrospective analysis was carried out of the outcome of 373 consecutive transplants performed at 38 European institutions between 1980 and 1988 and reported to the registry of the European Group for Blood and Marrow Transplantation. All transplants were carried out for first chronic phase of chronic myelogenous leukemia using unmanipulated marow cells from HLA-identical sibling donors. The probability of survival and leukemia-free survival at 8 years were 54% (95% CI: 49-59) and 47% (95% CI: 41-52) respectively. The probabilities of developing acute GVHD (II-IV) at 100 days and chronic GVHD at 4 years after transplant were 47% (95% CI: 41-53) and 52% (95% CI: 46-58) respectively. The probabilities of transplant-related mortality and leukemic relapse 8 years after BMT were 41% (95% CI: 36-48) and 19% (95% CI: 14-25), respectively. Transplant within 12 months of diagnosis was associated with reduced transplant-related mortality (34 vs 45%, P = 0.013) and resulted in improved leukemia-free survival (52 vs 44%, P = 0.03). The probability of relapse was significantly reduced in patients who developed chronic GVHD (RR = 0.33, P = 0.004). The probability of relapse occurring more than 2 years after transplant was increased more than five-fold in patients transplanted from a male donor (RR = 5.5, P = 0.006). Sixty-seven patients in hematologic remission were studied for residual disease by two-step RT/PCR for BCR-ABL mRNA and 61 (91%) tested negative. We conclude that bone marrow transplantation can induce long-term survival in approximately one-half of CML patients; the majority of survivors have no evidence of residual leukemia cells when studied by molecular techniques. The probability of late relapse is increased with use of a male donor. 相似文献