Viscoelastic Behavior of Synthesized Liquid Soaps and Surface Activity Properties of Surfactants

In the present work, a rheological study of liquid soaps prepared from different mixture of surfactants as a function of surfactant type and concentration was performed. The curves of shear stress vs. shear rate and viscosity vs. shear rate were recorded at constant temperature, 294 ± 0.1 K. The surface activity properties were also studied. The results of the study showed that values of surface tension, γ, were in the range 31–40 mN m⁻¹ and the critical micelle concentration (CMC), was of the order 10⁻⁴ mol L⁻¹. The calculated maximum surface excess, Γ_max, varied from 2.40 to 3.66 μmol m⁻², while minimum area per molecule, A_min, varied from 41.1 (for amphoterics) to 81.4 Ų (for nonionic surfactants). The standard free energy of micellization, −29.8 and −29.3 kJ mol⁻¹ for anionic and amphoteric surfactants, respectively, were while values for nonionic surfactants varied between −31.8 and − 30.3 kJ mol⁻¹. The free energy of adsorption, was the lowest for amphoteric surfactants (−37.9 kJ mol⁻¹), followed by anionics (−40.4 kJ mol⁻¹) and nonionics (−43.34 to −46.84 kJ mol⁻¹), indicating that micellization process is spontaneous in the examined medium. The synthetized liquid soaps show pseudoplastic behavior and they achieved pipe flow. The results of this research indicate that flow behavior was affected significantly by the ionic charge of the surfactant and the ionic strength of the formulation, suggesting that the flow behavior could be changed by manipulating the choice of the surfactant and salinity. The pH value of all liquid soaps examined were weakly acidic, in the range of 5.0–6.4.     

The revival of the traditional Bosnian wood dwellings

This paper presents the results of research regarding the possibilities of the revival of the traditional Bosnian single-family house. The architectural design, spatial organization, and connection with the natural environment make it a valuable example of residential architecture. The use of local natural materials such as wood, stone, and adobe brick, brings this typology back into the limelight. This study illustrates that due to the increasing need to save energy, the importance to use natural materials, and integration of sustainable design into architectural design processes, our contemporaries turn to past examples in order to reference benchmarks combined with cutting edge technology for buildings of the future. Because Bosnia has large wooded areas, and a policy goal is to use local resources, the research aim was to establish if modern prefabricated wooden houses can reproduce traditional Bosnian architecture while meeting the highest energy efficiency requirements. Many Bosnians fear that traditional Bosnian architecture will be lost due to new construction, therefore there is opposition against the harmonization of standards. The solutions for redesigning traditional Bosnian single-family homes to meet energy efficiency requirements were obtained using the “BuildOpt_VIE” dynamic thermal simulation software. It was used to find optimal solutions in building physics, along with several variations to be applied during construction of new buildings and reconstruction of existing buildings. This research sets a foundation for further developments in contemporary individual residences, optimized according to recent building physics standards. The purpose of this paper is inter alia to show that nothing stands in the way to harmonize technical guidelines and regulations in the field of energy-efficient buildings in Bosnia and Herzegovina (B&H), and the adaption of existing norms. Despite the EU requirements as mandated in the Energy Performance of Buildings Directive, the rich architecture of B&H has potential to fulfill the directive’s conditions. By selecting the best solutions from the rich architectural tradition of B&H and optimizing the architectural design and building physics, B&H takes a step towards EU integration. Here, we show the possibility to keep traditional architecture while achieving state of the art energy efficiency.     

An investigation of the usability of image-based CAPTCHAs using PROMETHEE-GAIA method

Multimedia Tools and Applications - Today, it is difficult to find an adequate Web site with a registration form that is not protected with some automated human proof test. One of the oldest...     

Multivariate-activity mining for molecular quasi-species in a glutathione transferase mutant library

A library of recombinant glutathione transferases (GSTs) generated by shuffling of DNA encoding human GST M1-1 and GST M2-2 was screened with eight alternative substrates, and the activities were subjected to multivariate analysis. Assays were made in lysates of bacteria in which the GST variants had been expressed. The primary data showed clustering of the activities in eight-dimensional substrate-activity space. For an incisive analysis, the rows of the data matrix, corresponding to the different enzyme variants, were individually scaled to unit length, thus accounting for different expression levels of the enzymes. The columns representing the activities with alternative substrates were subsequently individually normalized to unit variance and a zero mean. By this standardization, the data were adjusted to comparable orders of magnitude. Three molecular quasi-species were recognized by multivariate K-means and principal component analyses. Two of them encompassed the parental GST M1-1 and GST M2-2. A third one diverged functionally by displaying enhanced activities with some substrates and suppressed activities with signature substrates for GST M1-1 and GST M2-2. A fourth cluster contained mutants with impaired functions and was not regarded as a quasi-species. Sequence analysis of representatives of the mutant clusters demonstrated that the majority of the variants in the diverging novel quasi-species were structurally similar to the M1-like GSTs, but distinguished themselves from GST M1-1 by a Ser to Thr substitution in the active site. The data show that multivariate analysis of functional profiles can identify small structural changes influencing the evolution of enzymes with novel substrate-activity profiles.     

Curcumin Loaded PEGylated Nanoemulsions Designed for Maintained Antioxidant Effects and Improved Bioavailability: A Pilot Study on Rats

The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.     

Fatty Acid Amide Hydrolase Deficiency Is Associated with Deleterious Cardiac Effects after Myocardial Ischemia and Reperfusion in Mice

Ischemic cardiomyopathy leads to inflammation and left ventricular (LV) dysfunction. Animal studies provided evidence for cardioprotective effects of the endocannabinoid system, including cardiomyocyte adaptation, inflammation, and remodeling. Cannabinoid type-2 receptor (CB2) deficiency led to increased apoptosis and infarctions with worsened LV function in ischemic cardiomyopathy. The aim of our study was to investigate a possible cardioprotective effect of endocannabinoid anandamide (AEA) after ischemia and reperfusion (I/R). Therefore, fatty acid amide hydrolase deficient (FAAH)^−/− mice were subjected to repetitive, daily, 15 min, left anterior descending artery (LAD) occlusion over 3 and 7 consecutive days. Interestingly, FAAH^−/− mice showed stigmata such as enhanced inflammation, cardiomyocyte loss, stronger remodeling, and persistent scar with deteriorated LV function compared to wild-type (WT) littermates. As endocannabinoids also activate PPAR-α (peroxisome proliferator-activated receptor), PPAR-α mediated effects of AEA were eliminated with PPAR-α antagonist GW6471 i.v. in FAAH^−/− mice. LV function was assessed using M-mode echocardiography. Immunohistochemical analysis revealed apoptosis, macrophage accumulation, collagen deposition, and remodeling. Hypertrophy was determined by cardiomyocyte area and heart weight/tibia length. Molecular analyses involved Taqman^® RT-qPCR and immune cells were analyzed with fluorescence-activated cell sorting (FACS). Most importantly, collagen deposition was reduced to WT levels when FAAH^−/− mice were treated with GW6471. Chemokine ligand-2 (CCL2) expression was significantly higher in FAAH^−/− mice compared to WT, followed by higher macrophage infiltration in infarcted areas, both being reversed by GW6471 treatment. Besides restoring antioxidative properties and contractile elements, PPAR-α antagonism also reversed hypertrophy and remodeling in FAAH^−/− mice. Finally, FAAH^−/−-mice showed more substantial downregulation of PPAR-α compared to WT, suggesting a compensatory mechanism as endocannabinoids are also ligands for PPAR-α, and its activation causes lipotoxicity leading to cardiomyocyte apoptosis. Our study gives novel insights into the role of endocannabinoids acting via PPAR-α. We hypothesize that the increase in endocannabinoids may have partially detrimental effects on cardiomyocyte survival due to PPAR-α activation.     

满足再生要求产品的系统化生成方法

 描述了新的实践中获取的方法，用于产品开发中满足再生要求的系统化生产．所选例子及其步骤阐明了对资本货物和生产工业品所需的生产资料的再生方法．