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1.
Abidur Rahman Makoto Kobayashi Kotaro Sugimoto Yuta Endo Manabu Kojima Shigenori Furukawa Takafumi Watanabe Shu Soeda Yuko Hashimoto Keiya Fujimori Hideki Chiba 《International journal of molecular sciences》2021,22(7)
Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases. 相似文献
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Ken Kanazawa Shoji Yoshida Hidemi Shigekawa Shinji Kuroda 《Science and Technology of Advanced Materials》2015,16(1)
The reconstructed surface structure of the II–VI semiconductor ZnTe (110), which is a promising material in the research field of semiconductor spintronics, was studied by scanning tunneling microscopy/spectroscopy (STM/STS). First, the surface states formed by reconstruction by the charge transfer of dangling bond electrons from cationic Zn to anionic Te atoms, which are similar to those of IV and III–V semiconductors, were confirmed in real space. Secondly, oscillation in tunneling current between binary states, which is considered to reflect a conformational change in the topmost Zn–Te structure between the reconstructed and bulk-like ideal structures, was directly observed by STM. Third, using the technique of charge injection, a surface atomic structure was successfully fabricated, suggesting the possibility of atomic-scale manipulation of this widely applicable surface of ZnTe. 相似文献
4.
Shohei Minagawa Shoji Fujiwara Takeshi Hashimoto Takashi Hayashita 《International journal of molecular sciences》2021,22(9)
Cyclodextrins (CyDs) are water-soluble host molecules possessing a nanosized hydrophobic cavity. In the realm of molecular recognition, this cavity is used not only as a recognition site but also as a reaction medium, where a hydrophobic sensor recognizes a guest molecule. Based on the latter concept, we have designed a novel supramolecular sensing system composed of Zn(II)-dipicolylamine metal complex-based azobenzene (1-Zn) and 3A-amino-3A-deoxy-(2AS,3AS)-γ-cyclodextrin (3-NH2-γ-CyD) for sensing adenosine-5′-triphosphate (ATP). 1-Zn showed redshifts in the UV-Vis spectra and induced circular dichroism (ICD) only when both ATP and 3-NH2-γ-CyD were present. Calculations of equilibrium constants indicated that the amino group of 3-NH2-γ-CyD was involved in the formation of supramolecular 1-Zn/3-NH2-γ-CyD/ATP. The Job plot of the ICD spectral response revealed that the stoichiometry of 1-Zn/3-NH2-γ-CyD/ATP was 2:1:1. The pH effect was examined and 1-Zn/3-NH2-γ-CyD/ATP was most stable in the neutral condition. The NOESY spectrum suggested the localization of 1-Zn in the 3-NH2-γ-CyD cavity. Based on the obtained results, the metal coordination interaction of 1-Zn and the electrostatic interaction of 3-NH2-γ-CyD were found to take place for ATP recognition. The “reaction medium approach” enabled us to develop a supramolecular sensing system that undergoes multi-point interactions in water. This study is the first step in the design of a selective sensing system based on a good understanding of supramolecular structures. 相似文献
5.
A comparative study of DPSK and OOK WDM transmission over transoceanic distances and their performance degradations due to nonlinear phase noise 总被引:1,自引:0,他引:1
Mizuochi T. Ishida K. Kobayashi T. Abe J. Kinjo K. Motoshima K. Kasahara K. 《Lightwave Technology, Journal of》2003,21(9):1933-1943
We have compared experimentally the transmission performance of return-to-zero differential phase-shift keying (RZ-DPSK) with RZ-ON-OFF keying (OOK), nonreturn-to-zero differential phase-shift keying (NRZ-DPSK), and NRZ-OOK for 100/spl times/10-Gb/s transmission with a spectral efficiency of 0.22 b/s/Hz over transoceanic distances. The Q degradation of the RZ-DPSK after transmission over 9180 km was 3 dB greater than that of RZ-OOK. The experimental results clearly showed the major cause of degradation for DPSK is not cross-phase modulation but self-phase modulation. The calculated nonlinear phase noise, i.e., the Gordon-Mollenauer effect, agreed with the experimental results. A distributed-Raman-amplifier assisted erbium-doped-fiber-amplified transmission line acted well in reducing the nonlinear phase noise. 相似文献
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7.
Takashi Itoh Hitoe Habuchi Shoji Nitta Shuichi Nonomura 《Fullerenes, Nanotubes and Carbon Nanostructures》1994,2(2):181-187
Gas effusion spectra of fullerenes are studied. Three peaks of toluene are found in gas effusion spectra dp/dT-T. Two peaks of oxygen are found in gas effusion spectra dN/dt-T of samples kept in the air after annealing to avoid the toluene. These phenomena confirmed to be reversible are discussed by treating fullerenes as intercalated materials. 相似文献
8.
Sachiyuki Hasegawa Kazuyuki Shimizu Takeshi Kobayashi Masakazu Matsubara 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1985,35(1):33-42
Repeated batch operation using two fermentors (RBTF) to penicillin fermentation was demonstrated by computer simulation to improve productivity. Three operation modes were compared: chemostat, repeated batch operation using a single fermentor (RBSF) and RBTF; in each case account was made of the lag period before growth. The simulated fermentor performances were assessed on the basis of the penicillin productivity and concentration; the simulation was based on published batch fermentation data. It was shown that RBTF was superior to RBSF and chemostat. The advantage of RBTF increased as the lag period became greater. 相似文献
9.
Isamu Kobayashi 《Information Systems》1987,12(4):343-352
In classical database theory, relational calculus has long been used in expressing query formulae and integrity constraints. In fact, relational calculus formulae are much easier to deal with than first-order formulae when evaluating queries and validating database updates in the database environment. In deductive databases, however, first-order calculus is preferred because it is convenient when proof procedures are involved. Since both situations should coexist in advanced information systems, it is very desirable to devise a conversion procedure between relational calculus and first-order calculus. In this paper, interpretation of first-order formulae in the database environment is discussed first, then tuple calculus, an extension of relational calculus, is presented. This extension enables us to describe query formulae and general rules necessary in advanced information systems, in particular, dealing with complex objects. Finally, a conversion algorithm from first-order formulae into tuple calculus formulae is presented. Several application issues are also included. 相似文献
10.
Shoji Eguchi 《Fullerenes, Nanotubes and Carbon Nanostructures》1997,5(5):977-987
Recent progress of synthesis of C60 derivatives functionalized with hetero-cycles is reviewed, focusing attention on [4+2]- and [3+2] cycloaddition methodologies and oxidative heterocyclization. 相似文献