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排序方式: 共有173条查询结果,搜索用时 93 毫秒
1.
Translation elongation factor 1 alpha (EF-1 alpha) catalyzes the GTP-dependent binding of amino-acyl-tRNA to the ribosome. Previously, Tetrahymena 14-nm filament-associated protein was identified as EF-1 alpha [Kurasawa et al. (1992) Exp. Cell Res. 203, 251-258]. This and several other studies suggest that EF-1 alpha functions not only in translation but also in regulation of some part of the cytoskeleton. Tetrahymena EF-1 alpha bound to F-actin and induced bundling of F-actin. We investigated the effects of GTP/GDP and Ca2+/calmodulin on F-actin bundling activity of EF-1alpha. The presence of GTP, GDP, or guanylyl-imidodiphosphate (GMP-PNP) slightly decreased the amount of EF-1 alpha which bound to F-actin, but each had virtually no effect on the F-actin bundling activity. The formation of F-actin bundles by EF-1 alpha was Ca(2+)-insensitive. In the absence of Ca2+, calmodulin did not bind to EF-1 alpha and F-actin. On the other hand, in the presence of Ca2+, calmodulin directly bound to EF-1 alpha but did not have any serious influence on EF-1 alpha/F-actin binding. Under the conditions, electron microscopy demonstrated that Ca2+/calmodulin completely inhibited the F-actin bundling by EF-1 alpha. These results indicate that CA2+/calmodulin regulates the F-actin bundling activity of EF-1 alpha without inhibition of the binding between Ef-1 alpha and F-actin. 相似文献
2.
Abstract— We have developed a process to fabricate optical components, such as a lens, prism, or diffuser, directly on to a glass substrate. Processes include precision mastering by diamond cutting and multi‐layer photopolymer (2P) molding to realize flat surfaces and the integration of multiple components with an alignment within a few micrometers. 相似文献
3.
Shun'ichi Numata Shuuichi Oohara Koji Fujisaki Jun'Ichi Imaizumi Noriyuki Kinjo 《应用聚合物科学杂志》1986,31(1):101-110
The thermal expansion behavior for various aromatic polymides was investigated. Usally polymers, including polyimides, have high thermal expansion coefficients (3–6 × 10?5 K?1), compared with metals and ceramics. However, there are some polyimides which have very low thermal expansion coefficients below 1 × 10?5 K?1. This property was observed for the polymides obtained from pyromellitic dianhydride or 3,3′ 4,4′-biphenyltetracarboxylic dianhydride and aromatic diamines which were constituted of only benzene rings fused at para positions. It was proposed that their low thermal expansion coefficient related to the linearity in their polymer molecular skeltons. 相似文献
4.
5.
Tetsuya Ōgushi Gopal N. Suresha Yutaka Honjo Yoshihisa Ōzono Iwazo Kawano Tadashi Numata Yoshinori Hakuraku 《Journal of Low Temperature Physics》1987,69(5-6):451-457
We prepared La-Sr-Nb-O system samples both in bulk form and as a film, with sharp resistive transitions at temperatures of 228–323 K, depending on the annealing conditions. The sharp resistive transitions might be attributable to superconductivity. Magnetic ordering was observed over a narrow temperature range around 40 K for some of the samples. 相似文献
6.
The effect of particle agglomeration on sintering has been studied by slipcasting suspensions with pH values ranging from 2 to 11. The rate of densification has been found to depend on the degree of agglomeration. Complete dispersion of alumina primary particles has not been attained through adjustment of pH of suspensions, and agglomerates also remained in the best-dispersed suspension. Elimination of the agglomerates by sedimentation lowered the densification temperature of slip-cast compacts. The grain size-density curve is a function of temperature. High-density and small-grained sintered bodies were obtained by low-temperature long-time firing. 相似文献
7.
For the elucidation of the crystal structures of the two crystalline allomorphs of cellulose triacetate (CTA), namely CTA I and CTA II, two-dimensional (2D) solid-state through-bond 13C-13C and 1H-13C correlations NMR techniques applied to the two crystalline allomorphs of CTA. As a result, the 13C and 1H chemical shifts of the glucopyranose ring of CTA I and CTA II were completely assigned by the 2D NMR spectra of these allomorphs. On the 2D 13C-13C correlation spectrum of CTA II, two sets of the 13C-13C correlations from C1 to C6 were observed. This indicated that the CP/MAS 13C NMR spectrum of CTA II can be characterized by its overlapping of the 13C subspectra of two kinds of 2,3,6-triacetyl-anhydroglucopyranose units and that there are two magnetically non-equivalent sites in the unit cell of CTA II. In the case of CTA I, the numbers of respective 13C and 1H shifts of CTA I agreed with the those of the glucopyranose residue in the allomorph, strongly suggesting that the asymmetric unit of CTA I is only one glucose residue. In addition, conformational differences in the exocyclic C5-C6 bonds between CTA I and CTA II were strongly suggested by the notable differences in the 1H and 13C chemical shifts at the C6 sites of these allomorphs. 相似文献
8.
This paper presents a new method of partition, named-splitting, of a point set ind-dimensional space. Given a pointG in ad-dimensional simplexT, T(G;i) is the subsimplex spanned by G and the ith facet ofT. LetS be a set ofn points inT, and let be a sequence of nonnegative integers 1, ..., nd+1 satisfying
i=1
d+1
1=n The-splitter of (T, S) is a pointG inT such thatT(G;i) contains at least
i
points ofS in its closure for everyi=1, 2, ...,d + 1. The associated dissection is the re-splitting.The existence of a-splitting is shown for any (T, S) and, and two efficient algorithms for finding such a splitting are given. One runs inO(d2n logn + d3n) time, and the other runs inO(n) time if the dimensiond can be considered as a constant. Applications of re-splitting to mesh generation, polygonal-tour generation, and a combinatorial assignment problem are given. 相似文献
9.
K. Ohtani D. Numata T. Kikuchi M. Sun K. Takayama K. Togami 《International Journal of Impact Engineering》2006,33(1-12):555-565
This paper reports a result of hypervelocity impact experiments on cryogenically cooled aluminum alloys and a composite material. Experiments are carried out on a target palate at 122 K. Aluminum spheres at 1.95 km/s in 50 kPa air were impinged against the target plate at cryogenic temperature and the result was compared with room temperature target plates. Hypervelocity impact (HVI) processes were visualized with shadowgraph arrangement and recorded with high-speed video camera and to ensure the temperature dependence we compared HVI tests with metal target plates with AUTODYN 2D and SPH numerical simulations. We found that cryogenic impacts created slight differences of impact damage from room temperature ones, i.e., the shape and averaged diameters of HVI crater holes were less at cryogenic impacts. 相似文献
10.
Main‐chain smectic liquid crystalline polymer exhibiting unusually high thermal conductivity in an isotropic composite
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Shusuke Yoshihara Masatoshi Tokita Toshiaki Ezaki Mitsuru Nakamura Masashi Sakaguchi Kazuaki Matsumoto Junji Watanabe 《应用聚合物科学杂志》2014,131(6)
The thermal conductivity (TC) of an isotropic composite comprising of a main‐chain smectic liquid crystalline PB‐10 polyester and 50‐μm‐sized roughly spherical magnesium oxide (MgO) particles is investigated. The increase in the composite TC with higher MgO fractions is steeper than that expected by Bruggeman's theory for the TC of a polydomain PB‐10 polyester (0.52 W m?1 K?1). When the filler content is larger than 30 vol %, the composite TC approaches a value that can be explained only if the polyester functions as a matrix with 1.0 W m?1 K?1, which is five times as high as those of isotropic common polymers (0.2 W m?1 K?1). Such an unusually high TC for a polymer matrix is attributed to some polymer lamellae that lie parallel to the particle surface and are stacked toward neighboring particles, thus creating effective heat paths between the particles and a continuous thermal network in a composite. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014 , 131, 39896. 相似文献