Neighborhood disadvantage moderates associations of parenting and older sibling problem attitudes and behavior with conduct disorders in African American children.

Data from 296 sibling pairs (mean ages 10 and 13 years), their primary caregivers, and census records were used to test the hypothesis that African American children's likelihood of developing conduct problems associated with harsh parenting, a lack of nurturant-involved parenting, and exposure to an older sibling's deviance-prone attitudes and behavior would be amplified among families residing in disadvantaged neighborhoods. A latent construct representing harsh-inconsistent parenting and low levels of nurturant-involved parenting was positively associated with younger siblings' conduct disorder symptoms, as were older siblings' problematic attitudes and behavior. These associations were strongest among families residing in the most disadvantaged neighborhoods. Future research and prevention programs should focus on the specific neighborhood processes associated with increased vulnerability for behavior problems. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Coplanar waveguide radial line double stub and application to filter circuits

Coplanar waveguide (CPW) and grounded coplanar waveguide (GCPW) radial line double stub resonators are experimentally characterised with respect to stub radius and sector angle. A simple closed-form design equation, which predicts the resonance radius of the stub, is presented. Use of a double stub resonator as a lowpass filter or as a harmonic suppression filter is demonstrated and design rules are given.<>     

The importance of information localization in scene gist recognition.

People can recognize the meaning or gist of a scene from a single glance, and a few recent studies have begun to examine the sorts of information that contribute to scene gist recognition. The authors of the present study used visual masking coupled with image manipulations (randomizing phase while maintaining the Fourier amplitude spectrum; random image structure evolution [RISE]; J. Sadr & P. Sinha, 2004) to explore whether and when unlocalized Fourier amplitude information contributes to gist perception. In 4 experiments, the authors found that differences between scene categories in the Fourier amplitude spectrum are insufficient for gist recognition or gist masking. Whereas the global 1/f spatial frequency amplitude spectra of scenes plays a role in gist masking, local phase information is necessary for gist recognition and for the strongest gist masking. Moreover, the ability to recognize the gist of a target image was influenced by mask recognizability, suggesting that conceptual masking occurs even at the earliest stages of scene processing. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Environmental antigen-induced IL-13 responses are elevated among subjects with allergic rhinitis, are independent of IL-4, and are inhibited by endogenous IFN-gamma synthesis

Human immediate hypersensitivity diseases represent the most common example of chronic excessive Th2-like activation in developed nations. While IL-13 shares many functional properties with IL-4, the intensity and regulation of environmental Ag-stimulated IL-13 synthesis by allergic vs nonallergic individuals remain ill defined. Here, we examine the intensity of polyclonally and Ag-stimulated IL-13 production by PBMC of 20 subjects with seasonal allergic rhinitis and 20 healthy controls. Polyclonally driven IL-13 responses did not differ significantly (Mann-Whitney U test, p = 0.68). In contrast, the median CD4-dependent IL-13 response among atopics was markedly stronger than nonatopics in Ag-stimulated primary culture (p = 0.0031) and exhibited a strong correlation with IL-5 (r = 0.76, p = 0.0009), but not IL-4 (r = 0.14, p > 0.05), responses. IL-13 production was unaffected by blocking endogenous IL-4 or IL-5 activity or by addition of rIL-4 or rIL-5. In contrast, it was inhibited by low levels of rIFN-gamma and strongly enhanced upon addition of neutralizing anti-IFN-gamma mAb. Collectively, the data are consistent with a negative regulatory role for endogenous IFN-gamma synthesis in controlling the intensity of systemic IL-13 responses evoked in both atopic and nonatopic populations following exposure to common Ags. They also suggest that the elevated levels of IL-4 and IL-5 characteristic of type 2-dominated responses in vivo are without detectable impact on the maintenance of recall Ag-stimulated IL-13 production.     

A comparative study of UV active silane-grafted and ion-exchanged organo-clay for application in photocurable urethane acrylate nano- and micro-composites

A number of urethane acrylate nano- and micro-composites have been developed using both ion exchange and silane grafting chemistry. The organically modified clays which were used contained either methacrylate or acrylate functionalities which were capable of reacting with the acrylate groups in the urethane acrylate matrix. [2-(Acryloyloxy)ethyl]trimethyl-ammonium ion (AOETMA) or [2-(methacryloyloxy)ethyl]trimethylammonium ion (MAOTMA) were exchanged onto montmorillonite (MMT) as shown by an increase in the inter-gallery spacing of the MMT. Silane grafting was undertaken using [3-(acryloxy)propyl]dimethylmethoxysilane (APDMMS) or [3-(methacryloxy)propyl]dimethylmethoxysilane (MAPDMMS) and also showed an increase in inter-gallery spacing. The structures of the resulting urethane acrylate composites were characterized using X-ray diffraction (XRD) and transmission electron microscopy (TEM) and showed predominantly intercalated structures with some exfoliation (more evident in the silane-grafted systems). Dynamical mechanical thermal analysis (DMTA) showed a more significant increase in effective crosslink density (as measured from the plateau of the rubbery modulus) in the composites containing the reactive clays as compared to the unmodified clay, supporting the concept that the acrylate and methacrylate functionalities of the modified clays had reacted with the matrix. Other thermal and mechanical properties were also evaluated.     

Mosaic organization of DNA nucleotides

Long-range power-law correlations have been reported recently for DNA sequences containing noncoding regions. We address the question of whether such correlations may be a trivial consequence of the known mosaic structure ("patchiness") of DNA. We analyze two classes of controls consisting of patchy nucleotide sequences generated by different algorithms--one without and one with long-range power-law correlations. Although both types of sequences are highly heterogenous, they are quantitatively distinguishable by an alternative fluctuation analysis method that differentiates local patchiness from long-range correlations. Application of this analysis to selected DNA sequences demonstrates that patchiness is not sufficient to account for long-range correlation properties.     

Long-range correlation properties of coding and noncoding DNA sequences: GenBank analysis

An open question in computational molecular biology is whether long-range correlations are present in both coding and noncoding DNA or only in the latter. To answer this question, we consider all 33301 coding and all 29453 noncoding eukaryotic sequences--each of length larger than 512 base pairs (bp)--in the present release of the GenBank to dtermine whether there is any statistically significant distinction in their long-range correlation properties. Standard fast Fourier transform (FFT) analysis indicates that coding sequences have practically no correlations in the range from 10 bp to 100 bp (spectral exponent beta=0.00 +/- 0.04, where the uncertainty is two standard deviations). In contrast, for noncoding sequences, the average value of the spectral exponent beta is positive (0.16 +/- 0.05) which unambiguously shows the presence of long-range correlations. We also separately analyze the 874 coding and the 1157 noncoding sequences that have more than 4096 bp and find a larger region of power-law behavior. We calculate the probability that these two data sets (coding and noncoding) were drawn from the same distribution and we find that it is less than 10(-10). We obtain independent confirmation of these findings using the method of detrended fluctuation analysis (DFA), which is designed to treat sequences with statistical heterogeneity, such as DNA's known mosaic structure ("patchiness") arising from the nonstationarity of nucleotide concentration. The near-perfect agreement between the two independent analysis methods, FFT and DFA, increases the confidence in the reliability of our conclusion.     

A rationale for the TEI recommendations for feature-structure markup

In this paper, we concentrate on justifying the decisions we made in developing the TEI recommendations for feature structure markup. The first four sections of this paper present the justification for the recommended treatment of feature structures, of features and their values, and of combinations of features or values and of alternations and negations of features and their values. Section 5 departs briefly from the linguistic focus to argue that the markup scheme developed for feature structures is in fact a general-purpose mechanism that can be used for a wide range of applications. Section 6 describes an auxiliary document called a feature system declaration that is used to document and validate a system of feature-structure markup. The seventh and final section illustrates the use of the recommended markup scheme with two examples, lexical tagging and interlinear text analysis.Terry Langendoen is Professor and Head of the Department of Linguistics at The University of Arizona. He was Chair of the TEI Committee on Analysis and Interpretation. He received his PhD in Linguistics from the Massachusetts Institute of Technology in 1964, and held teaching positions at The Ohio State University and the City University of New York (Brooklyn College and the Graduate Center) before moving to Arizona in 1988. He is author, co-author, or co-editor of six books in linguistics, and of numerous articles.Gary Simons is Director of the Academic Computing Department of the Summer Institute of Linguistics, Dallas, TX. He served on the TEI Committee on Analysis and Interpretation. He received his PhD in Linguistics (with minor emphasis in Computer Science) from Cornell University in 1979. Before taking up his current position in 1984, he spent five years in the Solomon Islands doing field work with SIL. He is author, co-author, or co-editor of eight books in the fields of linguistics and linguistic computing.The initial feature-structure recommendations were formulated by the Analysis and Interpretation Committee at a meeting in Tucson, Arizona in March 1990, following suggestions by Mitch Marcus and Beatrice Santorini. The authors received valuable help in the further revision and refinement of the recommendations from Steven Zepp.     

Epinephrine doses delivered from auto-injectors stored at excessively high temperatures

Context: Prompt injection of epinephrine (adrenaline) from epinephrine auto-injectors (EAIs) is the primary treatment for anaphylaxis in out-of-hospital settings. Storage of EAIs at room temperature (25?°C) is advised; however, storage at excessively high temperatures sometimes occurs. To our knowledge, there are no previous publications on the doses of epinephrine ejected from EAIs after storage at such temperatures.

Objective: We examined the epinephrine doses delivered from activated EAIs stored constantly or cyclically at 70?°C.

Methods: Twenty-five in-date EAIs were stored constantly or cyclically at 70?°C (excessive heat) or 25?°C (controls) for 5?d or 10?d. EAIs were activated and the epinephrine doses in the ejected solutions were measured using HPLC-UV. The enantiomeric purity of epinephrine was also measured by HPLC-UV.

Results: Control EAIs ejected a volume of 0.300?±?0.006?mL containing 103.7?±?3.3% of labeled dose (LD). After 5?d or 10?d of constant storage at 70?°C and activation at 70?°C, EAIs ejected a volume of 0.367?±?0.008?mL containing 96.8?±?3.8% LD and 0.373?±?0.007?mL containing 77.7?±?3.3% LD, respectively. After 5?d of cyclic storage at 70?°C and cooling to 25?°C before activation, EAIs ejected a volume of 0.311?±?0.008?mL containing 87.2?±?1.9% LD. Under the experimental conditions of this study, the resultant chromatographic peaks of epinephrine solutions from all EAIs represented only the pure l-enantiomer of epinephrine.

Conclusion: EAIs should be stored under recommended conditions of the manufacturer. EAIs stored at excessively high temperatures cannot be used to treat humans while still hot, and when cooled, cannot be relied on to deliver the labeled epinephrine dose in anaphylaxis.     

Lipid domain structure of the plasma membrane revealed by patching of membrane components

Lateral assemblies of glycolipids and cholesterol, "rafts," have been implicated to play a role in cellular processes like membrane sorting, signal transduction, and cell adhesion. We studied the structure of raft domains in the plasma membrane of non-polarized cells. Overexpressed plasma membrane markers were evenly distributed in the plasma membrane. We compared the patching behavior of pairs of raft markers (defined by insolubility in Triton X-100) with pairs of raft/non-raft markers. For this purpose we cross-linked glycosyl-phosphatidylinositol (GPI)-anchored proteins placental alkaline phosphatase (PLAP), Thy-1, influenza virus hemagglutinin (HA), and the raft lipid ganglioside GM1 using antibodies and/or cholera toxin. The patches of these raft markers overlapped extensively in BHK cells as well as in Jurkat T-lymphoma cells. Importantly, patches of GPI-anchored PLAP accumulated src-like protein tyrosine kinase fyn, which is thought to be anchored in the cytoplasmic leaflet of raft domains. In contrast patched raft components and patches of transferrin receptor as a non-raft marker were sharply separated. Taken together, our data strongly suggest that coalescence of cross-linked raft elements is mediated by their common lipid environments, whereas separation of raft and non-raft patches is caused by the immiscibility of different lipid phases. This view is supported by the finding that cholesterol depletion abrogated segregation. Our results are consistent with the view that raft domains in the plasma membrane of non-polarized cells are normally small and highly dispersed but that raft size can be modulated by oligomerization of raft components.