Runoff capture through vegetative barriers and planting methodologies to reduce erosion,and improve soil moisture,fertility and crop productivity in southern Orissa,India

Use of perennial grasses as vegetative barriers to reduce soil erosion from farm and non-farm lands is increasing world-over. A number of perennial grasses have been identified for their soil conserving properties, but their effectiveness varies with location and method of planting. Installing vegetative barriers in combination with suitable mechanical measures, like bunds or trenches or both, on the appropriately spaced contours may enhance their conservation potential. Hence, the effect of vegetative barriers, viz., sambuta (Saccharum spp.)—a local grass, vetiver (Vetiveria zizanioides) and lemongrass (Cymbopogon citratus) planted in combination with trench-cum-bund, on runoff, soil loss, nutrient loss, soil fertility, moisture retention and crop yield in the rainfed uplands, was studied in Kokriguda watershed in southern Orissa, India through 2001–2005. However, runoff, soil and nutrient losses were studied for 2002, 2003 and 2004 only. Analysis of the experimental data revealed that on a 5% slope, the lowest average runoff (8.1%) and soil loss (4.0 Mg ha⁻¹) were observed in the sambuta + trench-cum-bund treatment followed by vetiver + trench-cum-bund (runoff 9.8%, soil loss 5.5 Mg ha⁻¹). Lemongrass permitted the highest runoff and soil loss. Further, the conservation effect of grass barriers was greater under bund planting than berm planting. Minimum organic C (50.02 kg ha⁻¹), available N (2.49 kg ha⁻¹) and available K (1.56 kg ha⁻¹) loss was observed under sambuta with bund planting. The next best arrester of the soil nutrients was vetiver planted on bund. Significantly better conservation of nutrients under sambuta and vetiver resulted in the soil fertility build-up. Soil moisture content was also higher in the sambuta and vetiver than lemongrass treated plots. Increase in the yield of associated finger millet (Eleusine coracana (L.) Gaertn.) due to vegetative barriers ranged from 18.04% for lemongrass to 33.67% for sambuta. Further, the sambuta and vetiver treated plots produced 13.23 and 11.86% higher yield, respectively, compared to the plots having lemongrass barrier (1.17 Mg ha⁻¹). Considering the conservation potential, and crop yield and soil fertility improvements, the sambuta barrier with trench-cum-bund is the best conservation technology for treating the cultivated land vulnerable to water erosion. Farmers also showed greater acceptance for the sambuta barrier as it is erect growing and available locally. Vetiver with-trench-cum bund can be the second best option.     

Culture prior to transplantation preserves the ultrastructural integrity of monkey pancreatic islets

Transplantation of pancreatic islets represents a promising way of curing type I diabetes (insulin-dependent diabetes mellitus). Culture enables the survival of endocrine tissue awaiting islet transplantation and reduces islet immunogenicity prior to xenografting. In this study, attempts were made to preserve the monkey islets in culture for 7 days and to study the ultrastructure by electron microscopy. The islets were isolated from monkey pancreas by the collagenase digestion method and were separated from acinar cells by dextran density gradient centrifugation. These islets were preserved in a humidified atmosphere of 5% carbon dioxide and 95% air for 7 days. The culture medium used was CMRL-1066. After 7 days of culture the islets were processed for light and electron microscopic studies, which revealed that the cultured islets were intact and maintained their structural integrity. Semi-thin sections of the cultured islets showed morphology with occasional structural alterations at the periphery. Dithizone staining of the cultured islets showed crimson red colour, proving that the islets were pure and without any exocrine contamination. Electron microscopy showed that the cultured islets had well-preserved alpha-, beta- and delta-cells. Different cell types of the monkey pancreatic islets were identified by the presence of their characteristic secretory granules. The ultrastructural characteristics present in hormone-synthesizing cells, i.e. rough-endoplasmic reticulum, Golgi apparatus, mitochondria and secretory granules, were observed as in native islets.     

Ferroptosis: A Potential Therapeutic Target in Acute Kidney Injury

Ferroptosis is a recently recognized form of nonapoptotic cell death that is triggered by reactive oxidative species (ROS) due to iron overload, lipid peroxidation accumulation, or the inhibition of phospholipid hydroperoxidase glutathione peroxidase 4 (GPX4). Recent studies have reported that ferroptosis plays a vital role in the pathophysiological process of multiple systems such as the nervous, renal, and pulmonary systems. In particular, the kidney has higher rates of O₂ consumption in its mitochondria than other organs; therefore, it is susceptible to imbalances between ROS and antioxidants. In ischemia/reperfusion (I/R) injury, which is damage caused by the restoring blood flow to ischemic tissues, the release of ROS and reactive nitrogen species is accelerated and contributes to subsequent inflammation and cell death, such as ferroptosis, as well as apoptosis and necrosis being induced. At the same time, I/R injury is one of the major causes of acute kidney injury (AKI), causing significant morbidity and mortality. This review highlights the current knowledge on the involvement of ferroptosis in AKI via oxidative stress.