Crystallographic electron microscopy

We present a family of techniques for the transmission electron microscope that generate surface zone-axis patterns. These patterns display the variation of the diffracted-beam intensity as a function of the angle of the incident electrons. The conditions of the experiments are those of reflection high-energy electron diffraction at near grazing incidence. The techniques are: surface convergent-beam diffraction, a surface analogue of the Tanaka method and a modified double-rocking scheme. Experimental results are presented for diffraction from surfaces of MgO and MoS₂. We anticipate that surface zone-axis patterns (surface ZAPs) will become established as an important tool for surface characterization, especially when used in conjunction with high-resolution surface imaging and surface energy loss spectroscopy; surface ZAPs may be expected to play, in surface analysis, a role analogous to that played by convergent-beam diffraction in normal transmission electron microscopy.     

Result Merging Technique for Answering XPath Query over XSLT Transformed Data

Caching stores the results of previously answered queries in order to answer succeeding queries faster by reusing these results. We propose two different approaches for using caches of XSLT transformed XML data in order to answer queries. The first approach checks whether or not a current query Q can be directly answered from the result of a previously answered query Qi stored in the cache. The new query is otherwise submitted to the source over the network, the answer of the query is determined, transmitted back to the client, and stored in the cache. The second approach determines only the intersection Q−Qi and integrates the result of Q−-Qi into the previous results in the cache, which requires applying a numbering scheme for the output of the XSLT stylesheet. We show by experimental results that the second approach can significantly speed up the answering time in comparison to the first approach, but is not significantly slower in few worst cases than the second approach.     

Structural sensitivity analysis of flexible multibody systems modeled with the floating frame of reference approach using the adjoint variable method

For the efficient analysis and optimization of flexible multibody systems, gradient information is often required. Next to simple and easy-to-implement finite difference approaches, analytical methods, such as the adjoint variable method, have been developed and are now well established for the sensitivity analysis in multibody dynamics. They allow the computation of exact gradients and require normally less computational effort for large-scale problems. In the current work, we apply the adjoint variable method to flexible multibody systems with kinematic loops, which are modeled using the floating frame of reference formulation. Thereby, in order to solve ordinary differential equations only, the equations of motion are brought into minimal form using coordinate partitioning, and the constraint equations at position and velocity level are incorporated in the adjoint dynamics. For testing and illustrative purposes, the procedure is applied to compute the structural gradient for a flexible piston rod of a slider–crank mechanism.     

On the implication problem for cardinality constraints and functional dependencies

In database design, integrity constraints are used to express database semantics. They specify the way by that the elements of a database are associated to each other. The implication problem asks whether a given set of constraints entails further constraints. In this paper, we study the finite implication problem for cardinality constraints. Our main result is a complete characterization of closed sets of cardinality constraints. Similar results are obtained for constraint sets containing cardinality constraints, but also key and functional dependencies. Moreover, we construct Armstrong databases for these constraint sets, which are of special interest for example-based deduction in database design.     

Stress Versus Coarticulation: Toward an Integrated Approach to Explicit Speech Segmentation.

Although word stress has been hailed as a powerful speech-segmentation cue, the results of 5 cross-modal fragment priming experiments revealed limitations to stress-based segmentation. Specifically, the stress pattern of auditory primes failed to have any effect on the lexical decision latencies to related visual targets. A determining factor was whether the onset of the prime was coarticulated with the preceding speech fragment. Uncoarticulated (i.e., concatenated) primes facilitated priming. Coarticulated ones did not. However, when the primes were presented in a background of noise, the pattern of results reversed, and a strong stress effect emerged: Stress-initial primes caused more pruning than non-initial-stress primes, regardless of the coarticulatory cues. The results underscore the role of coarticulation in the segmentation of clear speech and that of stress in impoverished listening conditions. More generally, they call for an integrated and signal-contingent approach to speech segmentation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Matisse: A System-on-Chip Design Methodology Emphasizing Dynamic Memory Management

MATISSE is a design environment intended for developing systems characterized by a tight interaction between control and data-flow behavior, intensive data storage and transfer, and stringent real-time requirements. Matisse bridges the gap from a system specification, using a concurrent object-oriented language, to an optimized embedded single-chip hardware/software implementation. Matisse supports stepwise exploration and refinement of dynamic memory management, memory architecture exploration, and gradual incorporation of timing constraints before going to traditional tools for hardware synthesis, software compilation, and inter-processor communication synthesis. With this approach, specifications of embedded systems can be written in a high-level programming language using data abstraction. Application of MATISSE on telecom protocol processing systems in the ATM area shows significant improvements in area usage and power consumption.     

Isolation of Decidual Macrophages and Hofbauer Cells from Term Placenta—Comparison of the Expression of CD163 and CD80

(1) Background: Placental immune cells are playing a very important role in a successful placentation and the prevention of pregnancy complications. Macrophages dominate in number and relevance in the maternal and the fetal part of the placenta. The evidence on the polarization state of fetal and maternal macrophages involved in both, healthy and pregnancy-associated diseases, is limited. There is no representative isolation method for the direct comparison of maternal and fetal macrophages so far. (2) Material and Methods: For the isolation of decidual macrophages and Hofbauer cells from term placenta, fresh tissue was mechanically dissected and digested with trypsin and collagenase A. Afterwards cell enrichment was increased by a Percoll gradient. CD68 is represented as pan-macrophage marker, the surface markers CD80 and CD163 were further investigated. (3) Results: The established method revealed a high cell yield and purity of the isolated macrophages and enabled the comparison between decidual macrophages and Hofbauer cells. No significant difference was observed in the percentage of single CD163⁺ cells in the distinct macrophage populations, by using FACS and immunofluorescence staining. A slight increase of CD80⁺ cells could be found in the decidual macrophages. Considering the percentage of CD80⁺CD163⁻ and CD80⁻CD163⁺ cells we could not find differences. Interestingly we found an increased number of double positive cells (CD80⁺CD163⁺) in the decidual macrophage population in comparison to Hofbauer cells. (4) Conclusion: In this study we demonstrate that our established isolation method enables the investigation of decidual macrophages and Hofbauer cells in the placenta. It represents a promising method for direct cell comparison, enzyme independently, and unaffected by magnetic beads, to understand the functional subsets of placental macrophages and to identify therapeutic targets of pregnancy associated diseases.