排序方式: 共有41条查询结果,搜索用时 281 毫秒
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Olena V. Moshynets Taras P. Baranovskyi Olga S. Iungin Nadiia P. Kysil Larysa O. Metelytsia Ianina Pokholenko Viktoria V. Potochilova Geert Potters Kateryna L. Rudnieva Svitlana Y. Rymar Ivan V. Semenyuta Andrew J. Spiers Oksana P. Tarasyuk Sergiy P. Rogalsky 《International journal of molecular sciences》2022,23(2)
The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here, we investigated how different biocides affect the release of eDNA from mature biofilms of two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles. The effect of four groups of biocides, alcohols, hydrogen peroxide, quaternary ammonium compounds, and the polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl), was evaluated using PA and SA biofilms. Most biocides, except for PHMG-Cl and 70% ethanol, caused substantial eDNA release, and PHMG-Cl was found to block biofilm development when used at concentrations of 0.5% and 0.1%. This might be associated with the formation of DNA–PHMG-Cl complexes as PHMG-Cl is predicted to bind to AT base pairs by molecular docking assays. PHMG-Cl was found to bind high-molecular DNA and plasmid DNA and continued to inactivate DNA on surfaces even after 4 weeks. PHMG-Cl also effectively inactivated biofilm-associated antibiotic resistance gene eDNA released by a pan-drug-resistant Klebsiella strain, which demonstrates the potential of a polymeric biocide as a new surface-active agent to combat the spread of antibiotic resistance in hospital settings. 相似文献
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Viktoriya Sokolova Sebastian Neumann Anna Kovtun Svitlana Chernousova Rolf Heumann Matthias Epple 《Journal of Materials Science》2010,45(18):4952-4957
Nanoparticles with an inner core of calcium phosphate, followed by layers of DNA and calcium phosphate and an outer layer of poly(ethyleneimine) (PEI) were prepared, characterized, and tested on different cell lines (HeLa, T24, and NIH3T3). A considerable increase in transfection efficiency was found for such nanoparticles, compared to the commercial reagent Polyfect® (a cationic dendrimer). The DNA is incorporated into the nanoparticles and protected from the attack by enzymes (nucleases) inside the cytoplasm of cells. The outer layer of PEI leads to electrosteric colloidal stabilization and gives a positive charge to the nanoparticle, which is helpful for the penetration through the negatively charged cell membrane. 相似文献
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Nataliya Shcherban Svitlana Filonenko Sergii Sergiienko Pavel Yaremov Mykola Skoryk Volodymir Ilyin Dmitry Murzin 《International Journal of Applied Ceramic Technology》2018,15(1):36-41
Samples of porous silicon carbide were obtained using sucrose or carbon and aerosil or silica mesoporous molecular sieves (SBA‐3, SBA‐15, KIT‐6 and MCF). Fibers content in silicon carbide samples is higher when the mesopore surface area of carbon materials derived from carbon‐silica composites is lower. Based on the found correlation between the morphology and porosity of SiC and mesopore surface area of the carbon component in the composites, a templating action of carbon in carbothermal reduction was suggested. 相似文献
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Yuriy Zakrevskyy Marcel Richter Svitlana Zakrevska Nino Lomadze Regine von Klitzing Svetlana Santer 《Advanced functional materials》2012,22(23):5000-5009
The light‐induced reversible switching of the swelling of microgel particles triggered by photo‐isomerization and binding/unbinding of a photosensitive azobenzene‐containing surfactant is reported. The interactions between the microgel (N‐isopropylacrylamide, co‐monomer: allyl acetic acid, crosslinker: N,N′‐methylenebisacrylamide) and the surfactant are studied by UV‐Vis spectroscopy, dynamic and electrophoretic light scattering measurements. Addition of the surfactant above a critical concentration leads to contraction/collapse of the microgel. UV light irradiation results in trans‐cis isomerization of the azobenzene unit incorporated into the surfactant tail and causes an unbinding of the more hydrophilic cis isomer from the microgel and its reversible swelling. The reversible contraction can be realized by blue light irradiation that transfers the surfactant back to the more hydrophobic trans conformation, in which it binds to the microgel. The phase diagram of the surfactant‐microgel interaction and transitions (aggregation, contraction, and precipitation) is constructed and allows prediction of changes in the system when the concentration of one or both components is varied. Remote and reversible switching between different states can be realized by either UV or visible light irradiation. 相似文献
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Kobozeva Alla A. Bobok Ivan I. Grygorenko Svitlana M. 《Journal of Signal Processing Systems》2020,92(1):55-69
Journal of Signal Processing Systems - The presented new method for detecting of clone areas in a digital image is effective in conditions of disturbing effects on the image that are additional to... 相似文献
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Remotely piloted air systems (RPASs) are increasingly used for data transmission in the space-air-ground integrated networks (SAGINs). That is why it is important to know the possibilities and ways of integrating existing space, air and terrestrial networks for effective interaction with RPASs. This paper studies the issues of ensuring the required quality of service during heavy traffic in SAGIN and the choice of the necessary data transmission modes for this. Models of communication channels were created, including line-of-sight transmission (Model 1), public terrestrial network (PTN) (Model 2), low-orbit satellite transmission (Model 3) and PTN plus satellite transmission (Model 4). The relationships between models' performance and traffic parameters, channel bandwidths, bit errors, lost packets and models' topology were investigated using NetCracker Professional 4.1 software. The dependencies of the average utilization on the transaction size and the time between transactions with different statistical distribution laws were analyzed. The effect of different bandwidths was investigated. The influence of the bit error rate and the probability of packet fail chance on the channel performance was studied. The traffic characteristics in all models were compared. 相似文献