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1.
Abidur Rahman Makoto Kobayashi Kotaro Sugimoto Yuta Endo Manabu Kojima Shigenori Furukawa Takafumi Watanabe Shu Soeda Yuko Hashimoto Keiya Fujimori Hideki Chiba 《International journal of molecular sciences》2021,22(7)
Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases. 相似文献
2.
H Kato T Takashima H Kishikawa S Emura K Ohmori 《Canadian Metallurgical Quarterly》1997,51(8):489-490
We studied urinary N-acetyl-beta-D-glucosaminidase (NAG) in the early stage of diabetic nephropathy in 27 non-insulin-dependent diabetes mellitus (NIDDM) patients with a microalbumin level below 20 mg on 24-hour urine sample. Microalbumin and NAG excretion were measured in 24-hour urine samples collected on three separate occasions within seven days of admission. Creatinine clearance was determined simultaneously. There was a significant negative correlation between the creatinine clearance and 24-hour urinary NAG (r = -0.38, p < 0.05). Elevation of urinary NAG may indicate decreased renal function during early stage NIDDM nephropathy. 相似文献
3.
Takuma Suzuki Hang-Ju Ko Agus Setiawan Jung-Jin Kim Koh Saitoh Masami Terauchi Takafumi Yao 《Materials Science in Semiconductor Processing》2003,6(5-6):519-521
We report the successful growth of Ga-polar GaN epilayers on O-polar ZnO templates pre-deposited on c-sapphire. Prior to GaN growth, NH3 is exposed onto the ZnO template. The polarity of the GaN layers is confirmed by etching of the surface and by conversion beam electron diffraction (CBED), while the O-polar ZnO is confirmed by CBED. It is suggested that the NH3 pre-exposure helps form a Zn3N2 layer, which possesses inversion symmetry and inverts the crystal from anion polar to cation polar. 相似文献
4.
Bonding of ceramics to metals was carried out by using thermal sprayed coatings as interlayer materials. This bonding method is referred as the thermal spray bonding. In the present study, Si3N4 and Al2O3 ceramics were bonded to SS41 mild steel using activated Ti-Cu multilayer coatings plasma-sprayed on the steel in a low pressure atmosphere. The bondability was estimated by SEM/EDX analysis and shear tests of the joints. The results were compared to those obtained using Ti-Cu multilayer foils as interlayers. When using Ti-Cu foils as interlayers, brittle Fe-Ti compound layers were formed in the joint area after long time heating at a bonding temperature of 900°C, which deteriorated the joint strength. In contrast with the activated interlayers made by plasma spraying, the Ti-Cu eutectic reaction took place uniformly at the joint immediately after heating to the bonding temperature. This improved the bondabilrty, and the resultant joints exhibited shear strengths of about 180 MPa. 相似文献
5.
T Nakajima K Miwa Y Ohmori T Sakabe M Fujii T Kajitani 《Canadian Metallurgical Quarterly》1996,23(9):1161-1168
A multi-center collaborative study was conducted in curatively resected gastric cancer patients at Stages II and III to compare oral 5-FU (Group A), oral Tegafur (Group B) and i.v. MMC + oral 5-FU (Group C). From May 1982 to April 1985, 1,012 cases were enrolled at 55 institutions. Some 138 (13.8%) were excluded, and 874 were analyzable. In the analysis of background factors, Group B had more cases with tumor of large diameter and advanced Stage. Adverse effects were relatively mild in all groups, and there was no problem in drug tolerance. Five-year survival rate was 67.6%, 62.4% and 68.6% in Groups A, B and C, respectively, reflecting no significant difference among them. It was 85.0%, 83.0% and 81.1% in Stage II and 52.5%, 51.0% and 59.0% in Stage III of Groups A, B and C, respectively. No significant difference was found, but Stage III of Group C showed a slightly higher survival rate. Supportive clinical study will be required to assess the usefulness of MMC as an introduction therapy. We found no difference in efficacy between 5-FU and Tegafur as maintenance therapy. The life-prolongation effect of fluoropyrimidines in comparison with surgery alone should be studied separately. 相似文献
6.
Eight-channel flat spectral response arrayed-waveguide multiplexer with asymmetrical Mach-Zehnder filters 总被引:1,自引:0,他引:1
K. Okamoto K. Takiguchi Y. Ohmori 《Photonics Technology Letters, IEEE》1996,8(3):373-374
An eight-channel flat spectral response arrayed-waveguide grating (AWG) multiplexer with asymmetrical Mach-Zehnder filters has been fabricated on the planar lightwave circuit (PLC). The monotonic spectral loss characteristics of AWG have been canceled by the opposite spectral response in the asymmetric MZ filter. The 1.5 dB bandwidth of 141 GHz and 3 dB bandwidth of 159 GHz are obtained for the 200 GHz channel spacing. The 1.5 dB bandwidth becomes 1.8 times wider than that of the conventional AWG. Crosstalks to neighboring and all other channels are less than -24 dB and the on-chip insertion losses range from 7.0 to 7.4 dB, respectively. 相似文献
7.
A WC-Co coating was sprayed by the high-velocity oxyfuel process using a feedstock of tungsten carbide clad with cobalt. The
structure of the sprayed coating was characterized by x-ray diffraction (XRD), differential scanning calorimetry (DSC), and
differential thermal analysis (DTA). It was found that an amorphous phase of Co-W-C ternary alloy observed as a large, broad
peak in the XRD pattern can be formed in the as-sprayed WC-Co coating. The DSC, DTA, and XRD analyses revealed that the amorphous
phase crystallized at a temperature of around 873 K to metallic cobalt, Co6W6C, and tungsten with appreciable precipitation of free carbon. The heat treatment of as-sprayed WC-Co coating at a high temperature
of 1173 K suggests that annealing at a temperature higher than about 1104 K will promote the reaction of tungsten and cobalt
with carbon to form the complex carbide C06W6C. 相似文献
8.
Zhongwen Wu Yubin Zheng Hongmao Yan Tomoki Nakamura Takafumi Nozawa Ryutoku Yosomiya 《大分子材料与工程》1989,173(1):163-181
Polymer blends of PEEK with PES were prepared by the solution blending method. Copolymers composed of PEEK and PES components were synthesized from these oligomers. The formation conditions exerted an influence over the molecular aggregation and the crystallization behaviors of the blend films and block copolymers which were examined by X-ray diffractometry and DSC analysis. As a result, phase-separation in the blend films was found when the formation temperature was high. The blend films formed at 340°C, quenched and annealed at 180°C, exhibited the same crystallization behavior as those of PEEK. In the case of the blend films formed at 300°C, the annealing of the films at 250°C was required to crystallize the blend films. The Tg of a copolymer with a PEEK component content of more than 50% tends to shift toward a higher temperature than the Tg of PEEK itself, and the Tm of the copolymer toward a lower temperature than that of PEEK ist. 相似文献
9.
Kwangryul Hwang Takafumi Noguchi Fuminiro Tomosawa 《Cement and Concrete Research》2004,34(12):697-2276
Based on experimental results concerning the compressive strength development of concrete containing fly ash, the authors derived an estimation equation for compressive strength development. The equation can express coefficient , which indicates the activity of fly ash as a binder, in the form of a function of age, fly-ash content, and Blaine specific surface area of fly ash.
This equation is capable of explaining the increases in the early strength due to fly ash in place of part of fine aggregate, the decreases in the early strength due to fly ash in place of part of cement, the increases in the long-term strength due to pozzolanic reaction, the relationship between the fly-ash replacement ratio and the ratio of strength increase/decrease, and the effect of fly ash's Blaine specific surface area on the strength. 相似文献
10.
A film of poly(l-lactic acid) (PLLA) consisting of highly oriented α crystals was uniaxially drawn by tensile force. The effects of the draw ratio (DR), draw temperature (Td), and draw stress on the crystal/crystal transformation from the α- to the β-form crystals were studied. At the initial stage of drawing, the highly oriented α crystals of the starting film transformed into a broader orientation distribution, and significant crystal disorder was introduced. Upon further drawing, the α crystals steadily transformed into β crystals with increasing the DR. For the drawing at a constant Td, the crystal transformation proceeded more efficiently at a higher draw rate and, hence, at a higher draw stress. Furthermore, for the drawing at a constant draw rate, the transformation proceeded with DR most efficiently for the tensile draw at a Td around 140 °C, although the draw stress increased with decreasing the Td. The present result combined with the previous finding in the drawing of PLLA by solid-state extrusion [Macromolecules 36 (2003) 3601] suggests that there is a Td of around 140 °C at which the crystal transformation proceeds most efficiently with DR, suggesting that there are two factors that have opposite effects on the efficiency of the crystal transformation with increasing the Td. However, as a result of the combined effects of the Td and DR on the crystal transformation and the ductility increase with the Td, an oriented film consisting predominantly of β crystals was obtained by tensile drawing at a Td in the range of 140-170 °C to the highest DR achieved at each Td. 相似文献