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1.
Lili Chen Nanami Fujisawa Masato Takanohashi Mazaya Najmina Koichiro Uto Mitsuhiro Ebara 《International journal of molecular sciences》2021,22(5)
This study demonstrates the rational fabrication of a magnetic composite nanofiber mesh that can achieve mutual synergy of hyperthermia, chemotherapy, and thermo-molecularly targeted therapy for highly potent therapeutic effects. The nanofiber is composed of biodegradable poly(ε-caprolactone) with doxorubicin, magnetic nanoparticles, and 17-allylamino-17-demethoxygeldanamycin. The nanofiber exhibits distinct hyperthermia, owing to the presence of magnetic nanoparticles upon exposure of the mesh to an alternating magnetic field, which causes heat-induced cell killing as well as enhanced chemotherapeutic efficiency of doxorubicin. The effectiveness of hyperthermia is further enhanced through the inhibition of heat shock protein activity after hyperthermia by releasing the inhibitor 17-allylamino-17-demethoxygeldanamycin. These findings represent a smart nanofiber system for potent cancer therapy and may provide a new approach for the development of localized medication delivery. 相似文献
2.
3.
Photoinduced Formation of Small Gold Particles in Silica Gels 总被引:2,自引:0,他引:2
AuCl− 4 ions in sol-gel-derived silica gels (Au/silica gels) were photoreduced by ultraviolet light irradiation at room temperature. In the presence of ethylene glycol in the silica gels, small Au particles were effectively formed by the irradiation. When the storage temperature of the gels was increased in the dark after the irradiation, the surface plas-mon resonance absorption peak of the Au particles was shifted to longer wavelengths, indicating that the Au particles became large. At a storage temperature below 9°C, Au particles could not be observed in the silica gels. The apparent activation energy of Au particle formation was estimated to be 17. 3 kcal·tmol−1 . 相似文献
4.
H Asahara K Fujisawa T Kobata T Hasunuma T Maeda M Asanuma N Ogawa H Inoue T Sumida K Nishioka 《Canadian Metallurgical Quarterly》1997,40(5):912-918
Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation. GFP has also proven to be an invaluable tool in monitoring trafficking and subcellular localization of protein. At the organ level and up, many exciting applications are rapidly emerging. The development of brighter GFP mutants with more robust folding properties has enabled better macroscopic visualization of GFP in whole leaves and plants. One interesting example has been the use of GFP to monitor virus movement in and among whole plants. GFP is also emerging as a powerful tool to monitor transgene movement and transgenic plants in the field. In a proof-of-concept study, tobacco was transformed with a modified version of the GFP gene controlled by a constitutive (35S) promoter. GFP expression in progeny plants ranged from 0% to 0.5%, and approximately 0.1% GFP was the minimal amount needed for unambiguous macroscopic detection. GFP is the first truly in vivo reporter system useful in whole plants, and we project its usefulness will increase even further as better forms of GFP genes become available. 相似文献
5.
Yoshihiro Iida Toshikatsu Tsuyuki Takao Mashima Takeo Takashima Kunito Okuyama 《亚洲传热研究》2002,31(1):28-41
This paper presents a series of experimental results on a passive augmentation technique of boiling heat transfer by supplying solid particles in liquid. A cylindrical heater 0.88 mm in diameter is placed in saturated water, in which a lot of mobile particles exist, and the nucleate and film boiling heat transfer characteristics are measured. Particle materials used were alumina, glass, and porous alumina, and the diameter ranged from 0.3 mm to 2.5 mm. Particles are fluidized by the occurrence of boiling without any additive power, and the heat transfer is augmented. The maximum augmentation ratio obtained in this experiment reaches about ten times the heat transfer coefficient obtained in liquid alone. The augmentation ratio is mainly affected by the particle material, diameter, and the height of the particle bed set at no boiling condition. The augmentation mechanism is discussed on the basis of the experimental results. © 2001 Scripta Technica, Heat Trans Asian Res, 31(1): 28–41, 2002 相似文献
6.
Masami Ikeda Susumu Fukumoto Hiroshi Takao Shinya Ohtsuka Eiichi Haginomori Masayuki Hikita 《Electrical Engineering in Japan》2002,141(4):16-24
This paper describes the dielectric breakdown characteristics of oil and oil‐impregnated paper for very fast transient (VFT) voltages. Blumlein circuits generate VFT voltages of 60 and 300 ns in a pulse width that simulates disconnecting switching surges in gas‐insulated switch gears. We measured the breakdown voltages of needle‐to‐plane, plane‐to‐plane oil gaps and several pieces of paper between plane electrodes for VFT and lightning impulse voltages. The measured data were formulated in V‐t characteristics and Weibull probability distributions. The inclination n of V‐t characteristics of insulating paper is 150, which is less than n = 13.7 of the plane‐to‐plane oil gap in the VFT time range. The shape parameters of Weibull distribution obtained in this study show that the scattering of breakdown voltages of paper is much less than that of oil. © 2002 Wiley Periodicals, Inc. Electr Eng Jpn, 141(4): 16–24, 2002; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.10043 相似文献
7.
Theoretical analysis based on the calculation of phase diagrams was employed for Fe-Si-Co and Fe-Si-Al ordering systems to
clarify the necessity for the occurrence of phase separation in Fe-base ternary ordering systems. The free energy of Fe-base
ternary ordering alloys where B2 and D03 ordered structures are formed is evaluated statistically using a pairwise interaction approximation up to second nearest
neighbours, taking into account not only the atomic interaction but also the magnetic interaction, based on the Bragg-Williams-Gorsky
model. The calculated phase diagrams are consistent with the experimentally obtained ones. The phase diagram calculation in
this work is useful to predict the equilibrium states of the ternary ordering systems. The phase separation in ordering alloys
is caused by the contribution of excess free energies due to ordering. The influences of ferromagnetism on the two-phase regions
are also demonstrated. 相似文献
8.
The objectives of this paper are to quantify the effect of Marangini convection on the absorption performance for the ammonia–water absorption process, and to visualize Marangoni convection that is induced by adding a heat transfer additive, n-octanol. A real-time single-wavelength holographic interferometer is used for the visualization using a He–Ne gas laser. The interface temperature is always the highest due to the absorption heat release near the interface. It was found that the thermal boundary layer (TBL) increased faster than the diffusion boundary layer (DBL), and the DBL thickness increased by adding the heat transfer additive. At 5 s after absorption started, the DBL thickness for 5 mass% NH3 without and with the heat transfer additive was 3.0 and 4.5 mm, respectively. Marangoni convection was observed near the interface only in the cases with heat transfer additive. The Marangoni convection was very strong just after the absorption started and it weakened as time elapsed. It was concluded that the absorption performance could be improved by increasing the absorption driving potential (xvb−xvi) and by increasing the heat transfer additive concentration. The absorption heat transfer was enhanced as high as 3.0–4.6 times by adding the heat transfer additive that generated Marangoni convection. 相似文献
9.
10.
JM Flanagan M Kataoka T Fujisawa DM Engelman 《Canadian Metallurgical Quarterly》1993,32(39):10359-10370
Deletion of 13 amino acids from the carboxyl terminus of staphylococcal nuclease (WTSNase delta) results in a denatured, partially unfolded molecule that lacks significant persistent secondary structure but is relatively compact and monomeric under physiological conditions [Shortle & Meeker (1989) Biochemistry 28, 936-944; Flanagan et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 748-752]. Because of these and other properties of the SNase delta polypeptide, it is a useful model system for investigating the conformation of the denatured state of a protein without using extreme temperature or solvent conditions. Moreover, since the modification is a carboxyl-terminal deletion, SNase delta may also resemble a transient state of the polypeptide chain as it emerges from a ribosome prior to its folding. In the present study, we have examined the sizes and conformations of mutated forms of SNase delta, using small-angle X-ray scattering and circular dichroism spectroscopy. Seven mutated forms were studied: four with single substitutions, two with double substitutions, and one triple substitution. When present in the full-length SNase, each of these mutated forms exhibited unusual behavior upon solvent or thermal denaturation. In the case of the truncated form (SNase delta), the small-angle scattering curves of the mutated forms fall into two classes: one resembling the scattering curve of compact native nuclease and the other having features consistent with those expected for an expanded coil-like polymer. In contrast, the scattering curve of WT SNase delta exhibits features intermediate between those observed for globular proteins and random polymers. The amino acid substitutions that gave rise to compact, native-like versions of SNase delta were all of the m--type (m-substitutions are predicted to decrease the size of the denatured state). Those which gave rise to versions of SNase delta that were more extended and coil-like than WT SNase delta were of the m+ type (m+ substitutions are predicted to increase the size of the denatured state). Estimates of the residual secondary structure present in WT SNase delta, as well as both the m+ and m-substituted versions of SNase delta, as determined by CD, suggest that the formation of secondary structure and compaction of the polypeptide chain occur concurrently. Our results show that single amino acid substitutions can radically alter the conformational distribution of a partially condensed polypeptide chain.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献