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排序方式: 共有796条查询结果,搜索用时 750 毫秒
1.
Dr. Yibin Zhang Dr. Shuai Xia Shulin Wan Tessa E. Steenwinkel Tara Vohs Prof. Rudy L. Luck Prof. Thomas Werner Prof. Haiying Liu 《Chembiochem : a European journal of chemical biology》2021,22(13):2282-2291
Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. 相似文献
2.
ABSTRACT Design and implementation of an effective dissemination programme for decentralised renewable energy system necessitate an accurate estimate of its utilisation potential. Hence, in this study, an attempt has been made to develop frameworks to estimate the utilisation potential of decentralised renewable energy systems in the state of Uttarakhand in India. Estimations imply large resource, technical and economic potentials of the domestic solar water heater, solar home system, solar lantern, family size biogas plant and improved biomass cookstove in Uttarakhand. With higher impact on the purchasing power of households, prevailing soft loan scheme has been found to be more appropriate than a capital subsidy for promoting the usage of decentralised renewable energy systems. 相似文献
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An optimization design model for surface termination of off-state semiconductor devices under reverse-bias conditions is presented. The multivariable design model is used to optimize the surface profile shapes for nonplanar devices and the doping profiles of material regions along the surface for planar devices. The effectiveness of the design model in reducing the peak surface electric field to below 50% of the bulk value is demonstrated for two examples of each type of device 相似文献
5.
A 73-year-old HTLV-1 infected male developed overt ATL following 3-years observation, and the clonality of HTLV-1 infected cells changed before overt ATL onset. 10 micrograms of DNA, extracted from mononuclear cells was digested with PstI, and the clonal proliferation of HTLV-1 infected cells was examined by Southern blotting with LTR probe. 4 bands were observed 3 years prior to ATL onset, and 2 different bands were detected 2 years and 6 months later. This suggests that in some ATL cases, the clonality of monoclonally proliferated HTLV-1 infected cells may be changeable before overt ATL onset. 相似文献
6.
Feng M. Scherrer D. Kruse J. Apostolakis P.J. Middleton J.R. 《Electron Device Letters, IEEE》1995,16(4):139-141
We present experimental evidence that the noise figure (NF) and associated gain equal to those achieved with GaAs pseudomorphic high electron mobility transistors (GaAs p-HEMT's) can also be accomplished by ion implanted GaAs metal-semiconductor field-effect transistors (GaAs MESFET's). These measured noise figure results as a function of low temperature for GaAs MESFET's and p-HEMT's clearly suggest that the transport properties of the two-dimensional electron gas in HEMT's and p-HEMT's do not make a significant contribution to the noise reduction at high frequency operation of these devices 相似文献
7.
An algorithm that acts as a preprocessor to the straight ray algebraic reconstruction technique (ART) algorithm, for the purpose of dealing with nonrectangular scanning geometries, is described. The mathematics of ART in its elementary form is reviewed, showing how the discretization of the rectangular scanning space defines quantities in the projection matrix of the algorithm. A triangular discretization approach for the scanning space that allows nonrectangular or twisted plane geometries to be addressed is proposed, and the effect of this approach on the projection matrix is discussed. An algorithm for determining the projection matrix under these discretization conditions is described, followed by some commentary on its computer implementation. The performance of this preprocessor algorithm, working with standard ART, in reconstructing various synthetic images is demonstrated 相似文献
8.
Handling delays in control systems is difficult and is of long-standing interest. It is well known that, given a finite-dimensional linear time-invariant (FDLTI) plant and controller forming a strictly proper stable feedback connection, closed-loop stability will be maintained under a small delay in the feedback loop, although most closed loop systems become unstable for large delays. One previously unsolved fundamental problem in this context is whether, for a given FDLTI plant, an arbitrarily large delay margin can be achieved using LTI control. Here, we adopt a frequency domain approach and demonstrate that, for a strictly proper real rational plant, there is a uniform upper bound on the delay that can be tolerated when using an LTI controller, if and only if the plant has at least one closed right half plane pole not at the origin. We also give several explicit upper bounds on the achievable delay margin, and, in some special cases, demonstrate that these bounds are tight. 相似文献
9.
It has been known for some time that real nonminimum phase zeros imply undershoot in the step response of linear systems. Bounds on such undershoot depend on the settling time demanded and the zero locations. In this note, we review such constraints for linear time invariant systems and provide new stronger bounds that consider simultaneously the effect of two real nonminimum phase zeros. Using the concept of zero dynamics, we extend these results to a class of nonlinear systems. 相似文献
10.