Modulation of apolipoprotein E-mediated plasma clearance and cell uptake of emulsion particles by cholesteryl ester

Cholesteryl ester, along with triglyceride (TG), is the major core component of plasma lipoproteins. We investigated the effect of core composition on the physical state and metabolic behavior of lipid emulsions, as model particles of lipoproteins. Fluorescence studies using 1,6-diphenylhexatriene analogs showed that although cholesteryl oleate (CO) significantly decreased core mobility, the surface rigidity of phosphatidylcholine (PC) monolayers was independent of core composition. When intravenously injected into rats, the increased amount of core CO tended to retard TG emulsion removal from plasma, and the initial clearance rate was correlated with the amount of apolipoprotein E (apoE) bound from plasma. In addition, PC liposomes with a similar emulsion particle size showed negligible binding of apoE and were cleared at a slower rate compared to all emulsions. Furthermore, the effect of CO on the binding behavior of apoE to the emulsion surface and the emulsion uptake by hepatocytes was assessed in vitro. Replacing core TG with CO was found to decrease the apoE binding capacity to emulsions markedly without changing the binding affinity and thereby to reduce the cell uptake of emulsion particles by HepG2 cells. These results indicate that the physical state of core lipids, which can be modulated by cO content, plays a role in emulsion metabolism through the alteration in apoE binding.     

Heat transport characteristics of flexible looped heat pipe under micro‐gravity condition

This paper presents the heat transport performance of a flexible looped heat pipe (FLHP) with the working fluid of ammonium under micro‐gravity condition. The evaporator of the FLHP has two coaxial wicks. The outer wick is made of PTFE with 1.2µm pore diameter and the inner one is made of polyethylene with 12µm pore diameter. The inside surface of the inner wick is axially grooved. The core of the inner wick is used as liquid reservoir so that reliability of the operation can be enhanced. The experiment revealed that the FLHP performed normally under micro‐gravity condition and had the predicted capillary limit. The FLHP showed good temperature control ability and was like a VCHP in the range of 150 to 240 W heat transfer rate. Liquid behavior observed in the visual module ensured the normal operation of the second wick and inner axial grooves under micro‐gravity condition. © 2003 Wiley Periodicals, Inc. Heat Trans Asian Res, 32(5): 381–390, 2003; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.10092     

Heat transfer performance of a flexible looped heat pipe using R134a as a working fluid: Proposal for a method to predict the maximum heat transfer rate of FLHP

This paper presents the heat transfer performance of a Flexible Looped Heat Pipe (FLHP) using R134a as a working fluid. In our evaluation system, an evaporator and a condenser are connected by long flexible tubes with a diameter of 3 mm, and the total piping length of this looped heat pipe system is approximately 7500 mm. We selected porous Teflon with an effective pore diameter of 2r_cw = 1.2 µm to overcome high gravitational heads. Elevation of the evaporator above the condenser ΔH = H_e ? H_c) was changed in three conditions [ Top heat mode (ΔH = +1 m), Horizontal mode (ΔH = 0), Bottom heat mode (ΔH = ?1 m)] considering the terrestrial application and the influence of gravity on the FLHP performance was investigated. FLHP provided high thermal transport capacities over long distances through small cross‐sectional flexible tubes compared with conventional heat pipes. We also proposed an analysis method to predict the maximum heat transfer rate of FLHP. © 2003 Wiley Periodicals, Inc. Heat Trans Asian Res, 32(4): 306–318, 2003; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.10093     

Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells

Recently, ferroptosis has gained scientists’ attention as an iron-related regulated necrosis. However, not many reports have investigated the effect of ferroptosis on bone. Therefore, with the present study, we assessed the effect of ferroptosis inhibition using ferrostatin-1 on the MC3T3-E1 pre-osteoblast cell. Cell images, cell viability, alkaline phosphatase activity test, alizarin red staining, and RUNX2 gene expression using real-time PCR were applied to investigate the effects of ferrostatin and erastin on MC3T3-E1 osteoblast cells. Erastin was used as a well-known ferroptosis inducer reagent. Erastin with different concentrations ranging from 0 to 50 µmol/L was used for inducing cell death. The 25 µmol/L erastin led to controllable partial cell death on osteoblast cells. Ferrostatin-1 with 0 to 40 µmol/L was used for cell doping and cell death inhibition effect. Ferrostatin-1 also displayed a recovery effect on the samples, which had already received the partially artificial cell death by erastin. Cell differentiation, alizarin red staining, and RUNX2 gene expression confirmed the promotion of the bone formation ability effect of ferrostatin-1 on osteoblast cells. The objective of this study was to assess ferrostatin-1’s effect on the MC3T3-E1 osteoblast cell line based on its ferroptosis inhibitory property.     

Presence of Apolipoprotein C-III Attenuates Apolipoprotein E-Mediated Cellular Uptake of Cholesterol-Containing Lipid Particles by HepG2 Cells

Apolipoprotein C-III (apoC-III) decreases the apolipoprotein E (apoE)-mediated uptake of lipoprotein remnants by the liver, and a high plasma concentration of apoC-III in VLDL is associated with hypertriglyceridemia and the risk of coronary heart disease. In this study, we prepared lipid emulsions containing triolein, phosphatidylcholine and cholesterol as model particles of lipoproteins, and examined the roles of apoC-III in apoE-mediated uptake of emulsions by HepG2 cells. Cholesterol in emulsion particles enhanced the apoE-mediated uptake via heparan sulfate proteoglycan and LDL receptor-related protein pathways. The amount of apoE bound to emulsion particles was increased by the presence of cholesterol at the particle surface, whereas cholesterol had no effect on the binding amount of apoC-III. Surface cholesterol alleviated the inhibitory effect of apoC-III on apoE incorporation into the emulsion surface. However, ApoC-III almost completely inhibited the apoE-mediated uptake of cholesterol-containing emulsions despite sufficient binding of apoE to emulsions. These findings suggest that apoC-III attenuates the binding of apoE to the lipoprotein surface and apoE-mediated cellular uptake of lipoprotein remnants. Furthermore, cholesterol may affect these functions of apoC-III and apoE involved in the clearance of lipoprotein remnants.     

Layered coding for ATM based video distribution systems

This paper discusses packet loss and its protection in an asynchronous transfer mode (ATM) based video distribution system. Packet losses in ATM based networks have such a great impact on the design of coding algorithms and network architectures that they should be exhaustively discussed and resolved. In this paper, first basic configuration of the ATM based video transmission system and its packet-loss protection schemes are discussed. The DCT based layered coding scheme with packet priority classification is proposed as an effective packet-loss protection scheme. Burstiness characteristics of the broadcast video sources are evaluated and modeled to clarify statistical multiplexing performance and packet-loss properties. The quality degradation caused by the packet losses is also evaluated by the SNR, and the superior performance of the proposed layered coding scheme is verified.     

Growth condition dependence of structural and electrical properties of Mg2Si layers grown on silicon substrates

Mg₂Si layers were grown on Si substrates by thermal treatment of the substrates in a Mg vapor, and the growth condition dependence of the structural and electrical properties of the layers was investigated. The layers were grown by an interdiffusion process between the deposited Mg atoms and the substrates. The structural and electrical properties of the layers depend on the combinations of the Si substrate and Mg source temperatures during the heat treatment. Any deviation from the isothermal treatment conditions causes degradation of the structural and/or electrical properties of the Mg₂Si layers. It was confirmed that the layers with the optimum structural and electrical properties were obtained when the layers were grown under isothermal growth conditions.     

Surface composition regulates clearance from plasma and triolein lipolysis of lipid emulsions

Sphingomyelin (SM) and cholesterol (Chol) are major surface lipid constituents of plasma lipoproteins. We investigated the effects of SM and Chol on the plasma clearance of lipid emulsions as a model for lipoprotein particles in rats. The presence of Chol facilitated the removal of emulsion particles from plasma, whereas SM delayed particle removal. Preinjection of lactoferrin, an inhibitor of the apolipoprotein E (apoE) receptor, revaled that the differences in clearance of emulsions were due to the differences in affinity for the apoE receptor. Measurement of apolipoprotein binding suggested that the balance of apoE and apoC (apoC-II and apoC-III) bound to emulsions caused the difference in plasma clearance of emulsion particles. That is to say, SM in the emulsion surface decreased binding of apoE, which led to a longer circulation of emulsion particles in plasma. Chol, on the other hand, decreased the ratio of apoC to apoE, which may have promoted emulsion uptake through the apoE receptor. We also examined in vitro lipolysis using immobilized lipoprotein lipase (LPL) in a heparin affinity column. Lipolysis rates were significantly reduced by the incorporation of SM into the emulsion surface, but not by the incorporation of Chol, indicating that SM in the lipoprotein surface is an important lipid component regulating LPL-mediated lipolysis. Our results suggest that the presence of SM and Chol in the lipoprotein surface plays an important role in the circulation behavior and LPL-mediated lipolysis of lipid emulsions through their effect on the selectivity of plasma protein binding.     

Application of Super High Definition Images in Telemedicine: System Requirements and Technologies for Teleradiology and Telepathology

It was recognized early on that the digitization of medical information would advance the efficiency of diagnostic technology. However, the digitization of image data, which makes up the majority of medical information, is dependent on advances in technologies such as input, processing, transmission, storage, and display. Insufficient advances in such technologies has effectively limited the digitization of image data for medical use. The result of this has been non-networked systems or LANs confined to a single hospital. Such isolated systems integrate only portions of digital medical images such as x-ray computer tomography (CT), magnetic resonance (MR), and computed radiography (CR).Fortunately, recent advances in the areas of super high definition image I/O, high-quality encoding, super high speed transmission, and high-capacity storage has turned the tide in favor of the digitization and networking of all medical information. This paper will focus on the digitization and networking of medical image information used within hospitals and provide a multifaceted study of the technologies necessary for these advances. This will allow us to discuss the present state of related technical developments and the level that has been attained so far. In addition, we have targeted image information that demands the highest level of quality (radiological and pathological images) for application in medical diagnosis using super high definition images, the image technology being developed by the authors of this paper. We will cover the concrete issues and approaches to solutions that must be investigated when building and networking a digital system.