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AA Gooley K Ou J Russell MR Wilkins JC Sanchez DF Hochstrasser KL Williams 《Canadian Metallurgical Quarterly》1997,18(7):1068-1072
Advances in protein database design and the software used to access the sequence data has led to progress in using protein attributes such as amino acid composition and peptide masses to identify proteins separated by two-dimensional electrophoresis. However, Edman degradation remains the principal technique for protein identification and it presents a significant bottleneck in the progress towards rapid protein identification. Simple modifications to the sequencing hardware, which automate the delivery of protein spots into the sequencer, and parallel sequencing of the protein spots represent a significant advance in the use of Edman degradation to rapidly generate the powerful protein attribute, an N-terminal sequence tag. 相似文献
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This report demonstrates how to calculate distances between points described in terms of latitude and longitude, using the Postal Code Conversion File (PCCF) and other files available from the Geography Division of Statistics Canada. Examples based on hospital data show how the results can be summarized in the form of tables, figures, or maps. Aerial distance to the nearest hospital is a crude but objective indicator of geographic accessibility to hospital services. Distances were calculated between enumeration area centroids for the population and blockface or enumeration area centroids for the hospitals. The median aerial distance to the nearest hospital facility in Canada was less than 3 km, while the mean distance was about 6 km. For teaching hospitals, the median aerial distance was a little less than 30 km, while the mean distance was a little more than 90 km. As most health files for administrative and research purposes contain postal codes, these techniques have potentially wide application. 相似文献
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David E. Wilkins 《Computational Intelligence》1988,4(3):373-380
Reasoning about actions necessarily involves tracking the truth of assertions about the world over time. The SIPE planning system retains the efficiency of the STRIPS assumption for this while enhancing expressive power by allowing the specification of a causl theory. Separation of knowledge about causality from knowledge about actions relieves operators of much of their representational burden and allows them to be applicable in a wide range of contexts. The implementation of causal theories is described, together with examples and evaluations of the system's expressive power and efficiency. 相似文献
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Paulussen-Hoogeboom Marja C.; Stams Geert Jan J. M.; Hermanns Jo M. A.; Peetsma Thea T. D. 《Canadian Metallurgical Quarterly》2007,43(2):438
This meta-analytic review (k = 62 studies; N = 7,613 mother-child dyads) shows that effect sizes for the association between child negative emotionality and parenting were generally small and were moderated by sample and measurement characteristics. The association between more child negative emotionality and less supportive parenting was relatively strong in lower socioeconomic status families, reversed in higher socioeconomic status families, and limited to studies with relatively high percentages of participants from ethnic minorities and studies using parent report to assess negative emotionality. Higher levels of child negative emotionality were associated with more restrictive control in samples with less than 75% 1st-born children, as well as in infants and preschoolers, and in studies using parent report or composite measures to assess both negative emotionality and restrictive parenting. Finally, more child negative emotionality was associated with less inductive control. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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A review of the development of the gel pond technology is presented. First, the emergence and growth of solar pond technology since the 1950's is described. The inherent problems encountered with the conventional salt gradient ponds are discussed, leading to the concept of the solar gel pond in which the salt gradient layer in the former is replaced by a transparent polymer gel. The major work in the first phase dealt with the experimental development of a polymer gel which met certain selection criteria. The criteria considered included transmissivity, stability of physical and chemical properties, high viscosity and other physical and optical properties. The gradual development of the polymer gel through an alternating process of testing and elimination and evaluation of relevant properties of the gel has been described. Modeling and optimization studies of the solar gel pond have been presented. Bansal and Kaushik's model for a salt gradient pond has been modified for a solar gel pond, and the results of the simulation are presented in a graphical form to serve as a quick reference for estimation of pond surface area, depth and flow rate for heat extraction depending on the extreme temperature required in the storage zone and the required heat load. Then, a cost-benefit economic analysis compares the economics of a solar gel pond with a conventional salt gradient pond. The construction of an experimental gel pond (18 m2) at The University of New Mexico is described, and the results of the study are summarized. Information on commercial scale ponds at Chamberino, New Mexico (110 m2), and in Albuquerque, New Mexico (400 m2), is provided. The review of the technology demonstrates the immense potential of the gel pond as a source of alternate energy for the years ahead. 相似文献
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Amirah Mohammad-Sidik Jian Sun Ryoung Shin Zhizhong Song Youzheng Ning Elsa Matthus Katie A. Wilkins Julia M. Davies 《International journal of molecular sciences》2021,22(2)
Extracellular ATP (eATP) has long been established in animals as an important signalling molecule but this is less understood in plants. The identification of Arabidopsis thaliana DORN1 (Does Not Respond to Nucleotides) as the first plant eATP receptor has shown that it is fundamental to the elevation of cytosolic free Ca2+ ([Ca2+]cyt) as a possible second messenger. eATP causes other downstream responses such as increase in reactive oxygen species (ROS) and nitric oxide, plus changes in gene expression. The plasma membrane Ca2+ influx channels involved in eATP-induced [Ca2+]cyt increase remain unknown at the genetic level. Arabidopsis thaliana Annexin 1 has been found to mediate ROS-activated Ca2+ influx in root epidermis, consistent with its operating as a transport pathway. In this study, the loss of function Annexin 1 mutant was found to have impaired [Ca2+]cyt elevation in roots in response to eATP or eADP. Additionally, this annexin was implicated in modulating eATP-induced intracellular ROS accumulation in roots as well as expression of eATP-responsive genes. 相似文献
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Lei Wang Charlotte E. Pelgrim Daniël H. Swart Guido Krenning Adrianus C. van der Graaf Aletta D. Kraneveld Thea Leusink-Muis Ingrid van Ark Johan Garssen Gert Folkerts Saskia Braber 《International journal of molecular sciences》2021,22(9)
Chronic obstructive pulmonary disease (COPD) caused by cigarette smoke (CS) is featured by oxidative stress and chronic inflammation. Due to the poor efficacy of standard glucocorticoid therapy, new treatments are required. Here, we investigated whether the novel compound SUL-151 with mitoprotective properties can be used as a prophylactic and therapeutic treatment in a murine CS-induced inflammation model. SUL-151 (4 mg/kg), budesonide (500 μg/kg), or vehicle were administered via oropharyngeal instillation in this prophylactic and therapeutic treatment setting. The number of immune cells was determined in the bronchoalveolar lavage fluid (BALF). Oxidative stress response, mitochondrial adenosine triphosphate (ATP) production, and mitophagy-related proteins were measured in lung homogenates. SUL-151 significantly decreased more than 70% and 50% of CS-induced neutrophils in BALF after prophylactic and therapeutic administration, while budesonide showed no significant reduction in neutrophils. Moreover, SUL-151 prevented the CS-induced decrease in ATP and mitochondrial mtDNA and an increase in putative protein kinase 1 expression in the lung homogenates. The concentration of SUL-151 was significantly correlated with malondialdehyde level and radical scavenging activity in the lungs. SUL-151 inhibited the increased pulmonary inflammation and mitochondrial dysfunction in this CS-induced inflammation model, which implied that SUL-151 might be a promising candidate for COPD treatment. 相似文献