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1.
Yoshihito Nakagawa Yukihiro Akao Kohei Taniguchi Akemi Kamatani Tomomitsu Tahara Toshiaki Kamano Naoko Nakano Naruomi Komura Hirokazu Ikuno Takafumi Ohmori Yasutaka Jodai Masahiro Miyata Mistuo Nagasaka Tomoyuki Shibata Naoki Ohmiya Ichiro Hirata 《International journal of molecular sciences》2015,16(1):1526-1543
Accumulating data indicates that certain microRNAs (miRNAs or miRs) are differently expressed in samples of tumors and paired non-tumorous samples taken from the same patients with colorectal tumors. We examined the expression of onco-related miRNAs in 131 sporadic exophytic adenomas or early cancers and in 52 sporadic flat elevated adenomas or early cancers to clarify the relationship between the expression of the miRNAs and the endoscopic morphological appearance of the colorectal tumors. The expression levels of miR-143, -145, and -34a were significantly reduced in most of the exophytic tumors compared with those in the flat elevated ones. In type 2 cancers, the miRNA expression profile was very similar to that of the exophytic tumors. The expression levels of miR-7 and -21 were significantly up-regulated in some flat elevated adenomas compared with those in exophytic adenomas. In contrast, in most of the miR-143 and -145 down-regulated cases of the adenoma-carcinoma sequence and in some of the de novo types of carcinoma, the up-regulation of oncogenic miR-7 and/or -21 contributed to the triggering mechanism leading to the carcinogenetic process. These findings indicated that the expression of onco-related miRNA was associated with the morphological appearance of colorectal tumors. 相似文献
2.
Minami Sakamoto Tomomitsu Miyagaki Hiroaki Kamijo Tomonori Oka Hikari Boki Naomi Takahashi-Shishido Hiraku Suga Makoto Sugaya Shinichi Sato 《International journal of molecular sciences》2021,22(15)
CD147, a transmembrane glycoprotein that belongs to the immunoglobulin superfamily, and cyclophilin A (CypA), one of the binding partners of CD147, are overexpressed in tumor cells and associated with the progression of several malignancies, including both solid and hematological malignancies. However, CD147 and CypA involvement in cutaneous T-cell lymphoma (CTCL) has not been reported. In this study, we examined CD147 and CypA expression and function using clinical samples of mycosis fungoides (MF) and Sézary syndrome (SS) and CTCL cell lines. CD147 and CypA were overexpressed by tumor cells of MF/SS, and CypA was also expressed by epidermal keratinocytes in MF/SS lesional skin. Serum CypA levels were increased and correlated with disease severity markers in MF/SS patients. Anti-CD147 antibody and/or anti-CypA antibody suppressed the proliferation of CTCL cell lines, both in vitro and in vivo, via downregulation of phosphorylated extracellular-regulated kinase 1/2 and Akt. These results suggest that CD147-CypA interactions can contribute to the proliferation of MF/SS tumor cells in both a autocrine and paracrine manner, and that the disruption of CD147-CypA interactions could be a new therapeutic strategy for the treatment of MF/SS. 相似文献
3.
Hiromasa Tanno Emi Kanno Suzuna Sato Yu Asao Mizuki Shimono Shiho Kurosaka Yukari Oikawa Shinyo Ishi Miki Shoji Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Hideki Yamamoto Keiko Ishii Yoshimichi Imai Masahiro Tachi Kazuyoshi Kawakami 《International journal of molecular sciences》2021,22(8)
Chronic infections are considered one of the most severe problems in skin wounds, and bacteria are present in over 90% of chronic wounds. Pseudomonas aeruginosa is frequently isolated from chronic wounds and is thought to be a cause of delayed wound healing. Invariant natural killer T (iNKT) cells, unique lymphocytes with a potent regulatory ability in various inflammatory responses, accelerate the wound healing process. In the present study, we investigated the contribution of iNKT cells in the host defense against P. aeruginosa inoculation at the wound sites. We analyzed the re-epithelialization, bacterial load, accumulation of leukocytes, and production of cytokines and antimicrobial peptides. In iNKT cell–deficient (Jα18KO) mice, re-epithelialization was significantly decreased, and the number of live colonies was significantly increased, when compared with those in wild-type (WT) mice on day 7. IL-17A, and IL-22 production was significantly lower in Jα18KO mice than in WT mice on day 5. Furthermore, the administration of α-galactosylceramide (α-GalCer), a specific activator of iNKT cells, led to enhanced host protection, as shown by reduced bacterial load, and to increased production of IL-22, IL-23, and S100A9 compared that of with WT mice. These results suggest that iNKT cells promote P. aeruginosa clearance during skin wound healing. 相似文献
4.
Effects of an endodontic auxiliary chemical substance on the bond strength of two methacrylate‐based endodontic sealers to dentin
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6.
Yuki Kawana Hiraku Suga Hiroaki Kamijo Tomomitsu Miyagaki Makoto Sugaya Shinichi Sato 《International journal of molecular sciences》2021,22(22)
Mycosis fungoides (MF) and Sézary syndrome (SS), the most common types of cutaneous T-cell lymphoma (CTCL), are characterized by proliferation of mature CD4+ T-helper cells. Patients with advanced-stage MF and SS have poor prognosis, with 5-year survival rates of 52%. Although a variety of systemic therapies are currently available, there are no curative options for such patients except for stem cell transplantation, and thus the treatment of advanced MF and SS still remains challenging. Therefore, elucidation of the pathophysiology of MF/SS and development of medical treatments are desired. In this study, we focused on a molecule called OX40. We examined OX40 and OX40L expression and function using clinical samples of MF and SS and CTCL cell lines. OX40 and OX40L were co-expressed on tumor cells of MF and SS. OX40 and OX40L expression was increased and correlated with disease severity markers in MF/SS patients. Anti-OX40 antibody and anti-OX40L antibody suppressed the proliferation of CTCL cell lines both in vitro and in vivo. These results suggest that OX40–OX40L interactions could contribute to the proliferation of MF/SS tumor cells and that the disruption of OX40–OX40L interactions could become a new therapeutic strategy for the treatment of MF/SS. 相似文献
7.
By using a 27-MHz piezoelectric quartz oscillator connected with a vector network analyzer, we obtained resonance frequency decreases (-DeltaFwater) and energy dissipation increases (DeltaDwater) during binding of biotinylated bovine serum albumin, biotinylated ssDNA, biotinylated dsDNA, and biotinylated pullulan to a NeutrAvidin-immobilized 27-MHz quartz crystal microbalance (QCM) plate in aqueous solution, as well as in the wet air phase (98% humidity, -DeltaFwet and DeltaDwet) and in the dry air phase (-DeltaFair and DeltaDair). -DeltaFwater indicates the total mass of the molecule, bound water, and vibrated water in aqueous solutions. -DeltaFwet indicates the total mass of the molecule and bound water. -DeltaFair simply shows the real mass of the molecule on the QCM. In terms of results, (-DeltaFwet)/(-DeltaFair) values indicated the bound water ratios per unit biomolecular mass were on the order of pullulan (2.1-2.2) > DNAs = proteins (1.4-1.6) > polystyrene (1.0). The (-DeltaFwater)/(-DeltaFair) values indicated the hydrodynamic water (bound and vibrated water) ratios per unit biomolecular mass were on the order of dsDNA (6.5) > ssDNA = pullulan (3.5-4.4) > proteins (2.4-2.5) > polystyrene (1.0). Energy dissipation parameters per unit mass in water (DeltaDwater/(-DeltaFair)) were on the order of pullulan > dsDNA > ssDNA > proteins > polystyrene. Energy dissipation in the wet and dry air phases (DeltaDwet and DeltaDair) were negligibly small, which indicates even these biomolecules act as elastic membranes in the air phase (without aqueous solution). We obtained a good linear relationship between [(-DeltaFwater)/(-DeltaFair) - 1], which is indicative of hydration and DeltaDwater/(-DeltaFair) of proteins. The aforementioned values suggest that the energy dissipation of proteins was mainly caused by hydration and that proteins themselves are elastic molecules without energy dissipation in aqueous solutions. On the contrary, plots in cases of denatured proteins, DNAs, and pullulans were relatively deviant toward the large hydration and energy dissipation from the theoretical line as perfect elastic materials, meaning that the large energy dissipation occurs because of viscoelastic properties of denatured proteins, linear DNAs, and pullulans in the water phase, in addition to energy dissipation due to the hydration of molecules. These two parameters could characterize various biomolecules with structural properties in aqueous solutions. 相似文献
8.
Takashi Onishi Masao Mizuno Tetsuya Yoshikawa Jun Munemasa Takao Inoue Aki Miyagaki Hisashi Nakamoto 《Journal of Electronic Materials》2010,39(10):2255-2266
The behavior of void growth in ultra-large-scale integration Cu interconnections has been investigated by grain-boundary diffusion
simulation to confirm the effectiveness of the high-pressure reflow process for suppressing stress-induced voiding. Void growth
was simulated by combining stress analysis and atomic flux analysis. The former was calculated by the finite-element method
(FEM), and the latter was calculated by an unusual FEM in which grain boundaries were defined as elements. From the results
of void growth analysis, we found that voids tend to disappear during the isochronal annealing step and that the void shrinkage
rate can be increased by two to three times by applying pressure of 150 MPa compared with normal-pressure annealing. From
the simulation results, it can be conjectured that the high-pressure reflow process is effective for eliminating voids in
via holes of Cu interconnections. 相似文献
9.
Takashi Onishi Masao Mizuno Tetsuya Yoshikawa Jun Munemasa Takao Inoue Aki Miyagaki Hisashi Nakamoto Takayuki Onoda 《Journal of Electronic Materials》2010,39(3):318-325
The reflow phenomenon of Cu interconnections deposited over via holes was analyzed by viscoelastic deformation simulation
using a finite-element method to clarify the embedding mechanism of Cu interconnections into via holes in a novel dual-damascene
fabrication technology, which combined sputter deposition and high-pressure annealing. Cu is considered to be a viscoelastic
body, and the stress distribution and deformation behavior of Cu interconnections were calculated. As a result, the deformation
behavior of the Cu interconnections exhibited close agreement between the calculated and the observed values. From our series
of results, we inferred that Cu interconnections are embedded into via holes by the creep deformation mechanism defined in
this study during the high-pressure annealing process. 相似文献
10.
Yasushi Ito Tomomitsu Hori Tsuneo Kusunoki Hiroshi Nomura Hirofumi Kondo 《Journal of the Society for Information Display》2014,22(8):419-428
SrGa2S4:Eu and CaS:Eu phosphors are useful to provide a wider color gamut to LCDs since their emissions are narrower, but they easily react with moisture. The phosphor sheet containing them was developed. The moisture barrier layers on it can separate them from a humid atmosphere. The LCD with the phosphor sheet could realize the wider color gamut of 90% NTSC‐xy, while the color gamut of the original LCD with the white LEDs containing YAG:Ce phosphor was 71% NTSC‐xy. The improvement of the brightness of the LCD with the phosphor sheet was studied. Encapsulates with a greater curvature on blue LEDs used with the phosphor sheet can increase the brightness. The brightness of the LCD with the phosphor sheet and blue LEDs with the spherical encapsulates of which the curvature = about 0.7 was estimated to reach to 85% of that of the original LCD. The simpler phosphor sheet with the aluminum film edge seals was also developed. It was confirmed that it has sufficient reliability during storage and operation at high temperature and high humidity and there is no difference in its reliability between the edge area and the center area. The importance of the moisture barrier layers which protect the SrGa2S4:Eu and CaS:Eu phosphors was confirmed. The phosphor sheet with the moisture barrier layers had better reliability than that with the PET layers. 相似文献