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排序方式: 共有514条查询结果,搜索用时 15 毫秒
1.
tRNA-guanine transglycosylases (TGT) are enzymes involved in the modification of the anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine-34 at the wobble position by the hypermodified base queuine. In prokaryotes TGT catalyzes the exchange of guanine-34 with the queuine (.)precursor 7-aminomethyl-7-deazaguanine (preQ1). The crystal structure of TGT from Zymomonas mobilis was solved by multiple isomorphous replacement and refined to a crystallographic R-factor of 19% at 1.85 angstrom resolution. The structure consists of an irregular (beta/alpha)8-barrel with a tightly attached C-terminal zinc-containing subdomain. The packing of the subdomain against the barrel is mediated by an alpha-helix, located close to the C-terminus, which displaces the eighth helix of the barrel. The structure of TGT in complex with preQ1 suggests a binding mode for tRNA where the phosphate backbone interacts with the zinc subdomain and the U33G34U35 sequence is recognized by the barrel. This model for tRNA binding is consistent with a base exchange mechanism involving a covalent tRNA-enzyme intermediate. This structure is the first example of a (beta/alpha)-barrel protein interacting specifically with a nucleic acid. 相似文献
2.
Polyarteritis nodosa is a rare disorder and a form of systemic vasculitis. A 48 year-old female was admitted to the hospital because menorrhagia and pelvic pain in February 1993. The patient underwent exploratory laparotomy resulting in a total hysterectomy and bilateral salpingo-oophorectomy for myoma of uterus and a right adnexal cystic mass. Histopathologic examination revealed left ovarian periarteritis nodosa. Further investigation and 9 months follow-up failed to show any systemic involvement. To our knowledge the isolated ovarian polyarteritis nodosa is the first case in the literature. 相似文献
3.
In the area of image retrieval from data bases and for copyright protection of large image collections there is a growing demand for unique but easily computable fingerprints for images. These fingerprints can be used to quickly identify every image within a larger set of possibly similar images. This paper introduces a novel method to automatically obtain such fingerprints from an image. It is based on a reinterpretation of an image as a Riemannian manifold. This representation is feasible for gray value images and color images. We discuss the use of the spectrum of eigenvalues of different variants of the Laplace operator as a fingerprint and show the usability of this approach in several use cases. Contrary to existing works in this area we do not only use the discrete Laplacian, but also with a particular emphasis the underlying continuous operator. This allows better results in comparing the resulting spectra and deeper insights in the problems arising. We show how the well known discrete Laplacian is related to the continuous Laplace-Beltrami operator. Furthermore, we introduce the new concept of solid height functions to overcome some potential limitations of the method. 相似文献
4.
The electrowinning of zinc from zinc chloride with a molten chloride electrolyte was investigated. The electrolysis of zinc chloride shows ohmic limitations. The energy consumption is to a large extent determined by the anodic reaction, the evolution of chlorine. The chlorine gas plume was visualised in a see-through furnace and images were analysed to determine the plume angle. This parameter serves as an input parameter for a model to determine the ohmic contributions of the electrolyte, the plume and a layer of chlorine bubbles sticking to the anode, as well as the plume velocity. The results indicate that the major contribution (60%) to the ohmic drop is due to a stagnant layer of bubbles growing and sticking to the anode, thereby decreasing the effective anode area by coverage. The plume velocity influences coverage characteristics to some extent, which influences the ohmic drop. 相似文献
5.
Gerhard Herold Uta Hesse Frank Wisst Carl Fahr Milena Fahr Gerhard Rogler Irmlind Geerling Eduard F. Stange 《Lipids》1994,29(11):735-745
High density lipoproteins (HDL) were recently demonstrated in an enterocyte model (CaCo-2 cells) to mediate reverse cholesterol
transport by retroendocytosis. The present study was carried out to define the role of the major HDL apoproteins (apo) A-I
and apo A-II in this pathway. HDL3 was fractionated by heparin affinity chromatography into the two main fractions containing either apo A-I only (fraction
A) or both apo A-I and apo A-II (fraction B). In addition, liposomes were reconstituted from purified apo A-I or apo A-II
and dimyristoyl phosphatidylcholine. The cell binding properties and cholesterol efflux potential were studied in the lipoprotein
fractions and the liposomes. Both fractions exhibited similar maximal binding capacities of 4427 (A) and 5041 (B) ng/mg cell
protein, but their dissociation constants differed (40.5 and 167.7 μg/mL, respectively). Fraction A induced cholesterol efflux
and stimulated cholesterol synthesis more than did fraction B. Fraction A mobilized both cellular free and esterified cholesterol,
whereas fraction B preferentially mobilized cholesteryl esters. Liposomes, containing either apo A-I or apo A-II, showed specific
binding, endocytosis and endosomal transport, and were released as intact particles. Apo A-I liposomes also mediated cholesterol
efflux. In conclusion, there is evidence that the HDL3 subfractions A and B, as well as reconstituted liposomes containing either apo A-I or apo A-II, were specifically bound and
entered a retroendocytosis pathway which was directly linked to cholesterol efflux. Quantitatively, the apo A-I subfraction
appeared to play the dominant role in normal enterocytes. The apo A-II content of fraction B was related to the mobilization
of cholesteryl esters. 相似文献
6.
7.
Alexander Paar Jürgen Reuter John Soldatos Kostas Stamatis Lazaros Polymenakos 《Applied Intelligence》2009,30(1):37-46
Recently, several standards have emerged for ontology markup languages that can be used to formalize all kinds of knowledge.
However, there are no widely accepted standards yet that define APIs to manage ontological data. Processing ontological information
still suffers from the heterogeneity imposed by the plethora of available ontology management systems. Moreover, ubiquitous
computing environments usually comprise software components written in a variety of different programming languages, which
makes it particularly difficult to establish a common ontology management API with programming language agnostic semantics.
We implemented an ontological Knowledge Base Server, which can expose the functionality of arbitrary off-the-shelf ontology
management systems via a formally specified and well defined API. A case study was carried out in order to demonstrate the
feasibility of our approach to use a formally specified ontology management API to implement a registry for ubiquitous computing
systems. 相似文献
8.
Benjamin Köhler Uta Preim Matthias Grothoff Matthias Gutberlet Katharina Fischbach Bernhard Preim 《Computer Graphics Forum》2016,35(1):32-43
Four‐dimensional phase‐contrast magnetic resonance imaging (4D PC‐MRI) allows the non‐invasive acquisition of time‐resolved, 3D blood flow information. Stroke volumes (SVs) and regurgitation fractions (RFs) are two of the main measures to assess the cardiac function and severity of valvular pathologies. The flow rates in forward and backward direction through a plane above the aortic or pulmonary valve are required for their quantification. Unfortunately, the calculations are highly sensitive towards the plane's angulation since orthogonally passing flow is considered. This often leads to physiologically implausible results. In this work, a robust quantification method is introduced to overcome this problem. Collaborating radiologists and cardiologists were carefully observed while estimating SVs and RFs in various healthy volunteer and patient 4D PC‐MRI data sets with conventional quantification methods, that is, using a single plane above the valve that is freely movable along the centerline. By default it is aligned perpendicular to the vessel's centerline, but free angulation (rotation) is possible. This facilitated the automation of their approach which, in turn, allows to derive statistical information about the plane angulation sensitivity. Moreover, the experts expect a continuous decrease of the blood flow volume along the vessel course. Conventional methods are often unable to produce this behaviour. Thus, we present a procedure to fit a monotonous function that ensures such physiologically plausible results. In addition, this technique was adapted for the usage in branching vessels such as the pulmonary artery. The performed informal evaluation shows the capability of our method to support diagnosis; a parameter evaluation confirms the robustness. Vortex flow was identified as one of the main causes for quantification uncertainties. 相似文献
9.
10.
Reuter M.; Stark R.; Hennig J.; Walter B.; Kirsch P.; Schienle A.; Vaitl D. 《Canadian Metallurgical Quarterly》2004,118(3):462
Although it is known that there are fundamental personality differences in the behavioral responses to emotional stimuli, traits have scarcely been investigated in this context by means of functional imaging studies. To maximize the variance with respect to personality, the authors tested 12 control subjects and 12 subjects who had sadomasochistic experiences with respect to the relationship between J. A. Gray's (1970) personality dimensions, the behavioral approach system (BAS) and the behavioral inhibition system (BIS), and brain activity in regions of interest. The BIS was associated with activity in numerous brain areas in response to fear, disgust, and erotic visual stimuli, whereas few associations could he detected between the BAS and brain activity in response to disgust and erotic stimuli. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献