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排序方式: 共有47条查询结果,搜索用时 15 毫秒
1.
Vadym Burchak Fritz Koch Leonard Siebler Sonja Haase Verena K. Horner Xenia Kempter G. Bjrn Stark Ute Schepers Alisa Grimm Stefan Zimmermann Peter Koltay Sandra Strassburg Günter Finkenzeller Filip Simunovic Florian Lampert 《International journal of molecular sciences》2022,23(14)
Introduction: Three-dimensional bioprinting can be considered as an advancement of the classical tissue engineering concept. For bioprinting, cells have to be dispersed in hydrogels. Recently, a novel semi-synthetic thiolene hydrogel system based on norbornene-functionalized gelatin (GelNB) and thiolated gelatin (GelS) was described that resulted in the photoclick hydrogel GelNB/GelS. In this study, we evaluated the printability and biocompatibility of this hydrogel system towards adipose-tissue-derived mesenchymal stem cells (ASCs). Methods: GelNB/GelS was synthesized with three different crosslinking densities (low, medium and high), resulting in different mechanical properties with moduli of elasticity between 206 Pa and 1383 Pa. These hydrogels were tested for their biocompatibility towards ASCs in terms of their viability, proliferation and differentiation. The extrusion-based bioprinting of ASCs in GelNB/GelS-high was performed to manufacture three-dimensional cubic constructs. Results: All three hydrogels supported the viability, proliferation and chondrogenic differentiation of ASCs to a similar extent. The adipogenic differentiation of ASCs was better supported by the softer hydrogel (GelNB/GelS-low), whereas the osteogenic differentiation was more pronounced in the harder hydrogel (GelNB/GelS-high), indicating that the differentiation fate of ASCs can be influenced via the adaption of the mechanical properties of the GelNB/GelS system. After the ex vivo chondrogenic differentiation and subcutaneous implantation of the bioprinted construct into immunocompromised mice, the production of negatively charged sulfated proteoglycans could be observed with only minimal inflammatory signs in the implanted material. Conclusions: Our results indicate that the GelNB/GelS hydrogels are very well suited for the bioprinting of ASCs and may represent attractive hydrogels for subsequent in vivo tissue engineering applications. 相似文献
2.
Santanu Mondal Andriy Durygin Vadym Drozd Jose Belisario Zhe Cheng 《Journal of the American Ceramic Society》2020,103(9):4876-4893
In this research, near fully dense single phase bulk multicomponent transition metal nitride (Nb1/3Ta1/3Ti1/3)N1−δ has been successfully synthesized from mixed commercial powders of NbN, TaN and TiN via reaction flash sintering technique. This was performed with an applied pressure of ~ 35 MPa at 25°C under a constant DC electric field (~24-32 V/cm). The flash event, which is the abrupt increase in current (up to ~ 25.2 A/mm2) and temperature, occurred without preheating. The threshold power dissipation on the sample right before the flash is ~ 0.7 W/mm3. The formation of single phase (Nb1/3Ta1/3Ti1/3)N1−δ random solid solution and its compositional uniformity were confirmed by XRD and EDS, respectively. The effects of ball milling duration and limiting current density on phase formation were studied. Simulation based on Joule heating provides an estimate of the ultimate sample temperature of ~ 1850°C. Vickers hardness of the obtained (Nb1/3Ta1/3Ti1/3)N1−δ is 17.6 ± 0.6 GPa, which is comparable to similarly flash sintered ingredient binary nitrides of TaN and NbN. TGA in air shows that the oxidation resistance of (Nb1/3Ta1/3Ti1/3)N1−δ is better than that of TaN and NbN but inferior to TiN. The study demonstrates that reaction flash sintering can be a highly efficient technique for synthesizing bulk multicomponent ceramics for both material fundamental investigations and application development. 相似文献
3.
V. D. Mochalin 《Measurement Techniques》2012,54(12):1396-1402
Methods for the amplitude modulation of the radiation flux reflected from a vibrating surface when the surface undergoes linear
and angular displacements, when the radiation is received by a photoreceiver, are analyzed. It is shown that the conversion
coefficient of the reflected radiation into an electrical signal is determined by the radiation flux gradient along the distance
in the direction towards the photoreceiver and in the directions of the reflection in the case of linear and angular displacements
of the probed surface, respectively. The considerable excess of possibilities of detecting vibrations of the objects from
the angular displacements of their surface is determined. 相似文献
4.
Lyci George Vadym DrozdAndriy Durygin Jiuhua ChenSurendra K. Saxena 《International Journal of Hydrogen Energy》2009
Mg2FeH6 was synthesized by ball milling MgH2 and Fe (2:1 molar ratio) mixture for 72 h followed by heating at 400 °C under H2 pressure. The hydride formation, its structure and homogeneity were investigated by scanning electron microscopy, X-ray diffraction, transmission electron microscopy and Raman spectroscopy. High pressure in situ synchrotron X-ray diffraction and Vienna ab initio simulation were used to determine bulk modulus of the sample. The bulk modulus of Mg2FeH6 was found to be 75.4(4) GPa by optimized experiment and 76.3 GPa by theoretical simulation. From high temperature in situ X-ray diffraction study the volumetric thermal expansion coefficient of Mg2FeH6 was found to be αv = 5.85(3) × 10−5 + 7.47(7) × 10−8 (T − To)/°C. Decomposition of Mg2FeH6 was observed at 425 °C and the decomposition products were Mg, Fe and H2. 相似文献
5.
We describe the application of a local discontinuous Galerkin method to the numerical solution of the three-dimensional shallow water equations. The shallow water equations are used to model surface water flows where the hydrostatic pressure assumption is valid. The authors recently developed a DG\linebreak method for the depth-integrated shallow water equations. The method described here is an extension of these ideas to non-depth-integrated models. The method and its implementation are discussed, followed by numerical examples on several test problems.This revised version was published online in July 2005 with corrected volume and issue numbers. 相似文献
6.
Nishad A. Phatak Shrinivas R. Kulkarni Vadym Drozd Surendra K. Saxena Liwei Deng Yingwei Fei Jingzhu Hu Wei Luo Rajeev Ahuja 《Journal of Alloys and Compounds》2008,463(1-2):220-225
Mn+1AXn compounds have gathered huge momentum because of its exciting properties. In this paper we report the synthesis of ternary layered ceramic Cr2GeC, a 211 Mn+1AXn compound by hot-pressing. Scanning electron microscopy and X-ray diffraction have been employed to characterize the new synthesized phase. High-pressure compressibility of Cr2GeC were measured using diamond anvil cell and synchrotron radiation at room temperature up to 48 GPa. No phase transformation was observed in the experimental pressure range. The bulk modulus of Cr2GeC calculated using the Birch–Murnaghan equation of state is 169 ± 3 GPa, with K′ = 3.05 ± 0.15. 相似文献
7.
8.
I. NeitzelV. Mochalin I. Knoke G.R. PalmeseY. Gogotsi 《Composites Science and Technology》2011,71(5):710-716
Mechanical properties and thermal conductivity of composites made of nanodiamond with epoxy polymer binder have been studied in a wide range of nanodiamond concentrations (0-25 vol.%). In contrast to composites with a low content of nanodiamond, where only small to moderate improvements in mechanical properties were reported before, the composites with 25 vol.% nanodiamond showed an unprecedented increase in Young’s modulus (up to 470%) and hardness (up to 300%) as compared to neat epoxy. A significant increase in scratch resistance and thermal conductivity of the composites were observed as well. The improved thermal conductivity of the composites with high contents of nanodiamond is explained by direct contacts between single diamond nanoparticles forming an interconnected network held together by a polymer binder. 相似文献
9.
10.
Guo S Solares SD Mochalin V Neitzel I Gogotsi Y Kalinin SV Jesse S 《Small (Weinheim an der Bergstrasse, Germany)》2012,8(8):1264-1269
The cantilever dynamics in single-frequency scanning probe microscopy (SPM) are undefined due to having only two output variables, which leads to poorly understood image contrast. To address this shortcoming, generalized phase imaging scanning probe microscopy (GP-SPM), based on broad band detection and multi-eigenmode operation, is developed and demonstrated on diamond nanoparticles with different functionalization layers. It is shown that rich information on tip-surface interactions can be acquired by separating the response amplitude, instant resonance frequency, and quality factor. The obtained data allow high-resolution imaging even in the ambient environment. By tuning the strength of tip-surface interaction, different surface functionalizations can be discerned. 相似文献